Effects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing

Clinical implications in oocyte freezing - A review article

S. U. Chen, Y. R. Lien, K. H. Chao, H. N. Ho, Y. S. Yang, T. Y. Lee

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Embryo freezing has been a successful practice, but oocyte cryopreservation formerly achieved poorer results. This was mainly due to low rates of survival, fertilization, and development. The major dissimilarities for oocytes to embryos are the character of the plasma membrane, the presence of cortical granules, at the metaphase of meiosis II with the spindle system. In addition, the oocytes must be fertilized by sperm at the appropriate time. To improve the survival rate, a refined slow freezing method with increased sucrose concentration would dehydrate oocytes more sufficiently. Vitrification is another approach to prevent ice crystal formation. Intracytoplasmic sperm injection is used to overcome possible zona hardening from the release of cortical granules. The microtubules of meiotic spindles are vulnerable to the thermal changes and would depolymerize. Cryopreserved oocytes exhibited serious disturbances of the microtubules immediately after thawing. Fertilization of oocytes with disorganized spindles could lead to chromosomal aneuploidy, digyny, and arrest of cleavage. After incubation, the microtubules would repolymerize in a time-dependent way. Normal fertilization and development of cryopreserved oocytes improved after appropriate incubation and timing of insemination, compatible with recovery of the spindles. With the improvement of survival, fertilization, and cleavage, oocyte cryopreservation would gain an imperative role.

Original languageEnglish
Pages (from-to)101-107
Number of pages7
JournalMolecular and Cellular Endocrinology
Volume202
Issue number1-2
DOIs
Publication statusPublished - Apr 28 2003
Externally publishedYes

Fingerprint

Spindle Apparatus
Thawing
Cryopreservation
Freezing
Oocytes
Vitrification
Ice
Cell membranes
Fertilization
Sucrose
Hardening
Microtubules
Recovery
Crystals
Embryonic Structures
Intracytoplasmic Sperm Injections
Insemination
Herpes Zoster
Meiosis
Aneuploidy

Keywords

  • Meiotic spindle
  • Oocyte cryopreservation
  • Slow freezing
  • Vitrification

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Effects of cryopreservation on meiotic spindles of oocytes and its dynamics after thawing : Clinical implications in oocyte freezing - A review article. / Chen, S. U.; Lien, Y. R.; Chao, K. H.; Ho, H. N.; Yang, Y. S.; Lee, T. Y.

In: Molecular and Cellular Endocrinology, Vol. 202, No. 1-2, 28.04.2003, p. 101-107.

Research output: Contribution to journalArticle

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