Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma

Masatsugu Kurokawa, Satoshi Konno, Satoshi Matsukura, Mio Kawaguchi, Koushi Ieki, Shintarou Suzuki, Miho Odaka, Shin Watanabe, Tetsuya Homma, Masayuki Sato, Hiroko Takeuchi, Takashi Hirose, Shau Ku Huang, Mitsuru Adachi

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Osteopontin (OPN) contributes to the development of T helper 1 (Th1)-mediated immunity and Th1-associated diseases. However, the role of OPN in bronchial asthma is unclear. Corticosteroids reduce airway inflammation, as reflected by the low eosinophil and T-cell counts, and the low level of cytokine expression. We investigated OPN production and the inhibitory effects of corticosteroids on OPN production in a murine model of allergic asthma. Methods: BALB/c mice were sensitized by intraperitoneal injections of ovalbumin (OVA) with alum. Some mice received daily injections of dexamethasone (DEX) or phosphate-buffered saline for 1 week. All OVA-challenged mice were exposed to aerosolized 1% OVA for 30 min an hour after these injections. After the OVA challenge, the mice were killed, and bronchoalveolar lavage (BAL) fluid and lung tissue were examined. Results: The levels of OPN protein in BAL fluid and OPN mRNA in lung tissue increased after OVA challenge. Most OPN-expressing cells were CD11c+ cells and some were T cells. DEX decreased the levels of OPN protein in the BAL fluid, and those of OPN mRNA and OPN protein in lung tissue. Conclusions: OPN may play an important role in allergic bronchial asthma. Corticosteroids inhibit OPN production in mice with allergic asthma. The beneficial effect of corticosteroids in bronchial asthma is partly due to their inhibitory effects on OPN production.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume149
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 2009

Keywords

  • Bronchial asthma
  • CD11c
  • Corticosteroid
  • Dendritic cells
  • Mouse
  • Osteopontin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Kurokawa, M., Konno, S., Matsukura, S., Kawaguchi, M., Ieki, K., Suzuki, S., Odaka, M., Watanabe, S., Homma, T., Sato, M., Takeuchi, H., Hirose, T., Huang, S. K., & Adachi, M. (2009). Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma. International Archives of Allergy and Immunology, 149(SUPPL. 1), 7-13. https://doi.org/10.1159/000210647