Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma

Masatsugu Kurokawa, Satoshi Konno, Satoshi Matsukura, Mio Kawaguchi, Koushi Ieki, Shintarou Suzuki, Miho Odaka, Shin Watanabe, Tetsuya Homma, Masayuki Sato, Hiroko Takeuchi, Takashi Hirose, Shau Ku Huang, Mitsuru Adachi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Osteopontin (OPN) contributes to the development of T helper 1 (Th1)-mediated immunity and Th1-associated diseases. However, the role of OPN in bronchial asthma is unclear. Corticosteroids reduce airway inflammation, as reflected by the low eosinophil and T-cell counts, and the low level of cytokine expression. We investigated OPN production and the inhibitory effects of corticosteroids on OPN production in a murine model of allergic asthma. Methods: BALB/c mice were sensitized by intraperitoneal injections of ovalbumin (OVA) with alum. Some mice received daily injections of dexamethasone (DEX) or phosphate-buffered saline for 1 week. All OVA-challenged mice were exposed to aerosolized 1% OVA for 30 min an hour after these injections. After the OVA challenge, the mice were killed, and bronchoalveolar lavage (BAL) fluid and lung tissue were examined. Results: The levels of OPN protein in BAL fluid and OPN mRNA in lung tissue increased after OVA challenge. Most OPN-expressing cells were CD11c+ cells and some were T cells. DEX decreased the levels of OPN protein in the BAL fluid, and those of OPN mRNA and OPN protein in lung tissue. Conclusions: OPN may play an important role in allergic bronchial asthma. Corticosteroids inhibit OPN production in mice with allergic asthma. The beneficial effect of corticosteroids in bronchial asthma is partly due to their inhibitory effects on OPN production.

Original languageEnglish
Pages (from-to)7-13
Number of pages7
JournalInternational Archives of Allergy and Immunology
Volume149
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Jun 1 2009
Externally publishedYes

Fingerprint

Osteopontin
Adrenal Cortex Hormones
Asthma
Ovalbumin
Bronchoalveolar Lavage Fluid
Lung
Dexamethasone
T-Lymphocytes
Messenger RNA
Injections
Proteins
Intraperitoneal Injections
Eosinophils
Immunity

Keywords

  • Bronchial asthma
  • CD11c
  • Corticosteroid
  • Dendritic cells
  • Mouse
  • Osteopontin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Kurokawa, M., Konno, S., Matsukura, S., Kawaguchi, M., Ieki, K., Suzuki, S., ... Adachi, M. (2009). Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma. International Archives of Allergy and Immunology, 149(SUPPL. 1), 7-13. https://doi.org/10.1159/000210647

Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma. / Kurokawa, Masatsugu; Konno, Satoshi; Matsukura, Satoshi; Kawaguchi, Mio; Ieki, Koushi; Suzuki, Shintarou; Odaka, Miho; Watanabe, Shin; Homma, Tetsuya; Sato, Masayuki; Takeuchi, Hiroko; Hirose, Takashi; Huang, Shau Ku; Adachi, Mitsuru.

In: International Archives of Allergy and Immunology, Vol. 149, No. SUPPL. 1, 01.06.2009, p. 7-13.

Research output: Contribution to journalArticle

Kurokawa, M, Konno, S, Matsukura, S, Kawaguchi, M, Ieki, K, Suzuki, S, Odaka, M, Watanabe, S, Homma, T, Sato, M, Takeuchi, H, Hirose, T, Huang, SK & Adachi, M 2009, 'Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma', International Archives of Allergy and Immunology, vol. 149, no. SUPPL. 1, pp. 7-13. https://doi.org/10.1159/000210647
Kurokawa, Masatsugu ; Konno, Satoshi ; Matsukura, Satoshi ; Kawaguchi, Mio ; Ieki, Koushi ; Suzuki, Shintarou ; Odaka, Miho ; Watanabe, Shin ; Homma, Tetsuya ; Sato, Masayuki ; Takeuchi, Hiroko ; Hirose, Takashi ; Huang, Shau Ku ; Adachi, Mitsuru. / Effects of corticosteroids on osteopontin expression in a murine model of allergic asthma. In: International Archives of Allergy and Immunology. 2009 ; Vol. 149, No. SUPPL. 1. pp. 7-13.
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abstract = "Background: Osteopontin (OPN) contributes to the development of T helper 1 (Th1)-mediated immunity and Th1-associated diseases. However, the role of OPN in bronchial asthma is unclear. Corticosteroids reduce airway inflammation, as reflected by the low eosinophil and T-cell counts, and the low level of cytokine expression. We investigated OPN production and the inhibitory effects of corticosteroids on OPN production in a murine model of allergic asthma. Methods: BALB/c mice were sensitized by intraperitoneal injections of ovalbumin (OVA) with alum. Some mice received daily injections of dexamethasone (DEX) or phosphate-buffered saline for 1 week. All OVA-challenged mice were exposed to aerosolized 1{\%} OVA for 30 min an hour after these injections. After the OVA challenge, the mice were killed, and bronchoalveolar lavage (BAL) fluid and lung tissue were examined. Results: The levels of OPN protein in BAL fluid and OPN mRNA in lung tissue increased after OVA challenge. Most OPN-expressing cells were CD11c+ cells and some were T cells. DEX decreased the levels of OPN protein in the BAL fluid, and those of OPN mRNA and OPN protein in lung tissue. Conclusions: OPN may play an important role in allergic bronchial asthma. Corticosteroids inhibit OPN production in mice with allergic asthma. The beneficial effect of corticosteroids in bronchial asthma is partly due to their inhibitory effects on OPN production.",
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AU - Ieki, Koushi

AU - Suzuki, Shintarou

AU - Odaka, Miho

AU - Watanabe, Shin

AU - Homma, Tetsuya

AU - Sato, Masayuki

AU - Takeuchi, Hiroko

AU - Hirose, Takashi

AU - Huang, Shau Ku

AU - Adachi, Mitsuru

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