Abstract

Recently, the term tensotaxis was proposed to describe the phenomenon that tensile stress or strain affects cell migration. Even so, less attention has been paid to the effects of compressive stress on cell behavior. In this study, by using an injection-molded method combined with photoelastic technology, we developed residual stress gradient-controlled poly-L-lactide discs. After culturing NIH-3T3 fibroblasts on the stress gradient substrate, the cell distributions for high- and low-stress regions were measured and compared. Our results showed that there were significantly more cells in the low-compressive stress region relative to their high-stress analogs (p <0.05). In addition, NIH-3T3 fibroblasts in the low-compressive stress region expressed more abundant extensive filopodia. These findings provide greater insight into the interaction between cells and substrates, and could serve as a useful reference for connective tissue development and repair.

Original languageEnglish
Pages (from-to)1273-1279
Number of pages7
JournalMedical and Biological Engineering and Computing
Volume47
Issue number12
DOIs
Publication statusPublished - Dec 2009

Fingerprint

Fibroblasts
Compressive stress
Residual stresses
Pseudopodia
Cell Communication
Connective Tissue
Cell Movement
Tensile strain
Substrates
Tensile stress
Technology
Injections
Repair
Tissue

Keywords

  • Fibroblast
  • Mechanotaxis
  • PLLA
  • Residual stress
  • Substrate

ASJC Scopus subject areas

  • Biomedical Engineering
  • Computer Science Applications
  • Medicine(all)

Cite this

Effects of compressive residual stress on the morphologic changes of fibroblasts. / Lin, Shu Li; Yang, Jen Chang; Ho, Kuo Ning; Wang, Chau Hsiang; Yeh, Chien Wu; Huang, Haw Ming.

In: Medical and Biological Engineering and Computing, Vol. 47, No. 12, 12.2009, p. 1273-1279.

Research output: Contribution to journalArticle

Lin, Shu Li ; Yang, Jen Chang ; Ho, Kuo Ning ; Wang, Chau Hsiang ; Yeh, Chien Wu ; Huang, Haw Ming. / Effects of compressive residual stress on the morphologic changes of fibroblasts. In: Medical and Biological Engineering and Computing. 2009 ; Vol. 47, No. 12. pp. 1273-1279.
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