Effects of boldine on mouse diaphragm and sarcoplasmic reticulum vesicles isolated from skeletal muscle

Jaw Jou Kang, Yu Wen Cheng

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The effects of boldine [(S)-2,9-dihydroxy-1,10-dimethoxyaporphine], a major alkaloid in the leaves and bark of boldo (Peumus boldus Mol.), on skeletal muscle were studied using mouse diaphragm and isolated sarcoplasmic reticulum membrane vesicles. Boldine, at 10-200 μM, has little effect on the muscle-evoked twitches; however, the ryanodine-induced contracture was potentiated dose-dependently. At higher concentrations of 300 μM, boldine by itself induced muscle contracture of two phases, which were caused by the influx of extracellular Ca2+ and induction of Ca2+ release from the internal Ca2+ storage site, the sarcoplasmic reticulum, respectively When tested with isolated sarcoplasmic reticulum membrane vesicles,boldine dose-dependently induced Ca2+ release from actively loaded sarcoplasmic reticulum vesicles isolated from skeletal muscle of rabbit or rat which was inhibited by ruthenium red, suggesting that the release was through the Ca2+ release channel, also known as the ryanodine receptor. Boldine also dose-dependently increased apparent [3H]-ryanodine binding with the EC50 value of 50 μM. In conclusion, we have shown that boldine could sensitize the ryanodine receptor and induce Ca2+ release from the internal Ca2+ storage site of skeletal muscle.

Original languageEnglish
Pages (from-to)18-21
Number of pages4
JournalPlanta Medica
Volume64
Issue number1
DOIs
Publication statusPublished - 1998
Externally publishedYes

Fingerprint

sarcoplasmic reticulum
diaphragm
Sarcoplasmic Reticulum
Diaphragms
Diaphragm
Muscle
skeletal muscle
Skeletal Muscle
calcium
mice
Ryanodine
Ryanodine Receptor Calcium Release Channel
contracture
ryanodine receptors
Contracture
Peumus
Peumus boldus
Membranes
dosage
Ruthenium Red

Keywords

  • Boldine
  • Ca release
  • Monimiaceae
  • Peumus boldus
  • Sarcoplasmic reticulum

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Effects of boldine on mouse diaphragm and sarcoplasmic reticulum vesicles isolated from skeletal muscle. / Kang, Jaw Jou; Cheng, Yu Wen.

In: Planta Medica, Vol. 64, No. 1, 1998, p. 18-21.

Research output: Contribution to journalArticle

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