Effects of baicalin on the gene expression of surfactant protein A (SP-A) in lung adenocarcinoma cell line H441

Kur Ta Cheng, Yu Chen Huang, Yin Shiou Lin, Borcherng Su, Chung Ming Chen

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Pulmonary surfactant, a complex lipoprotein, is secreted by alveolar type 11 cells. It lies at the alveolar air-fluid interface and prevents alveolar collapse by reducing surface tension. The high incidence of respiratory distress syndrome (RDS) in premature infants results principally from a deficiency of pulmonary surfactant. Surfactant protein A (SP-A) is the most abundant surfactant protein and reduces surface tension at the alveolar air-liquid interface in lung cells. In this study, RT-PCR and Western blot analyses of SP-A were performed to evaluate the biological activity of baicalin, a Chinese medicine prescribed extensively for preventing miscarriage. In in vitro experiments, lung adenocarcinoma cell line H441 was cultured with baicalin in varying concentrations and for varying lengths of time. The results show that the expression of SP-A gene was positively affected by baicalin in dose-dependent and time-course manners. The maximal expression of the SP-A gene, 1.7-fold greater than control, is induced at 150 nM of baicalin treated for 48 h.

Original languageEnglish
Pages (from-to)300-304
Number of pages5
JournalPlanta Medica
Volume69
Issue number4
DOIs
Publication statusPublished - Apr 1 2003

Fingerprint

Pulmonary Surfactant-Associated Protein A
adenocarcinoma
Gene expression
surfactants
lungs
Cells
cell lines
Gene Expression
Pulmonary Surfactants
Cell Line
gene expression
Surface Tension
Surface tension
proteins
Genes
Air
Alveolar Epithelial Cells
surface tension
Spontaneous Abortion
Bioactivity

Keywords

  • Baicalin
  • Gene expression
  • Lamiaceae
  • Scutellaria baicalensis
  • Surfactant protein A
  • Traditional Chinese medicine

ASJC Scopus subject areas

  • Plant Science
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Effects of baicalin on the gene expression of surfactant protein A (SP-A) in lung adenocarcinoma cell line H441. / Cheng, Kur Ta; Huang, Yu Chen; Lin, Yin Shiou; Su, Borcherng; Chen, Chung Ming.

In: Planta Medica, Vol. 69, No. 4, 01.04.2003, p. 300-304.

Research output: Contribution to journalArticle

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AU - Chen, Chung Ming

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AB - Pulmonary surfactant, a complex lipoprotein, is secreted by alveolar type 11 cells. It lies at the alveolar air-fluid interface and prevents alveolar collapse by reducing surface tension. The high incidence of respiratory distress syndrome (RDS) in premature infants results principally from a deficiency of pulmonary surfactant. Surfactant protein A (SP-A) is the most abundant surfactant protein and reduces surface tension at the alveolar air-liquid interface in lung cells. In this study, RT-PCR and Western blot analyses of SP-A were performed to evaluate the biological activity of baicalin, a Chinese medicine prescribed extensively for preventing miscarriage. In in vitro experiments, lung adenocarcinoma cell line H441 was cultured with baicalin in varying concentrations and for varying lengths of time. The results show that the expression of SP-A gene was positively affected by baicalin in dose-dependent and time-course manners. The maximal expression of the SP-A gene, 1.7-fold greater than control, is induced at 150 nM of baicalin treated for 48 h.

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