Effects of B cell depletion on T cell allogeneic Immune responses: A strategy to reduce allogeneic sensitization

Meng-Kun Tsai, Hsiung-Fei Chien, Mei-Ching Tzeng, Po-Huang Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: B cell depletion has been employed to treat antibody-mediated organ transplantation rejection, although the effects on cellular immune responses have not been extensively investigated. Methods: A model of B cell depletion used SCID/beige mice reconstituted with BALB/c splenocytes either depleted of B cells (BD) or not (BN). BD and B/N mice received C57BL/6 skin grafts and were sacrificed after 6 weeks (BD-S6 and BN-S6). Results: Recall proliferative responses of BD-S6 splenocytes to C57BL/6 were significantly reduced compared to BN-S6, and central memory T cells' proportions (CD4+CD44+CD62L+ or CD8+CD44+CD62L+) were significantly decreased in BD-S6 spleens. Recall IFN-γ production by BD-S6 splenocytes was significantly reduced compared to BN-S6 splenocytes (p = 0.0028). Survival times of C57BL/6 heart grafts were significantly longer in SCID/beige mice reconstituted with BD-S6 splenocytes (8.5 ± 1.1 days) than for SCID/beige reconstituted with BN-S6 splenocytes (6.0 ± 1.1 days; p = 0.0006). Under cyclosporine therapy, C57BL/6 heart survival was significantly longer for SCID/beige reconstituted with BD-S6 splenocytes (17.5 ± 6.4 days) than those reconstituted with BN-S6 splenocytes (6.2 ± 1.5 days; p < 0.0001). Conclusion: B cell depletion during allogeneic sensitization decreased memory T cells and recalls IFN-γ production and reduced second-set allograft rejection. © 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalTransplant Immunology
Volume21
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

S 6
B-Lymphocytes
T-Lymphocytes
SCID Mice
Transplants
Graft Rejection
Organ Transplantation
Inbred C57BL Mouse
Cellular Immunity
Cyclosporine
Allografts
Spleen

Keywords

  • Allogeneic sensitization
  • B cell depletion
  • Memory T cells
  • CD4 antigen
  • CD8 antigen
  • cyclosporin
  • gamma interferon
  • Hermes antigen
  • L selectin
  • alloimmunity
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • article
  • B lymphocyte
  • cardiac graft rejection
  • cell proliferation
  • cellular immunity
  • controlled study
  • cytokine production
  • female
  • graft survival
  • heart graft
  • memory T lymphocyte
  • mouse
  • nonhuman
  • priority journal
  • skin graft
  • spleen cell
  • survival time
  • T lymphocyte
  • Animals
  • Antigens, CD
  • B-Lymphocytes
  • Cell Proliferation
  • Graft Rejection
  • Immunologic Memory
  • Immunosuppression
  • Interferon-gamma
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, SCID
  • Skin Transplantation
  • T-Lymphocytes
  • Transplantation Chimera
  • Transplantation Conditioning

Cite this

Effects of B cell depletion on T cell allogeneic Immune responses: A strategy to reduce allogeneic sensitization. / Tsai, Meng-Kun; Chien, Hsiung-Fei; Tzeng, Mei-Ching; Lee, Po-Huang.

In: Transplant Immunology, Vol. 21, No. 4, 2009, p. 215-220.

Research output: Contribution to journalArticle

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title = "Effects of B cell depletion on T cell allogeneic Immune responses: A strategy to reduce allogeneic sensitization",
abstract = "Background: B cell depletion has been employed to treat antibody-mediated organ transplantation rejection, although the effects on cellular immune responses have not been extensively investigated. Methods: A model of B cell depletion used SCID/beige mice reconstituted with BALB/c splenocytes either depleted of B cells (BD) or not (BN). BD and B/N mice received C57BL/6 skin grafts and were sacrificed after 6 weeks (BD-S6 and BN-S6). Results: Recall proliferative responses of BD-S6 splenocytes to C57BL/6 were significantly reduced compared to BN-S6, and central memory T cells' proportions (CD4+CD44+CD62L+ or CD8+CD44+CD62L+) were significantly decreased in BD-S6 spleens. Recall IFN-γ production by BD-S6 splenocytes was significantly reduced compared to BN-S6 splenocytes (p = 0.0028). Survival times of C57BL/6 heart grafts were significantly longer in SCID/beige mice reconstituted with BD-S6 splenocytes (8.5 ± 1.1 days) than for SCID/beige reconstituted with BN-S6 splenocytes (6.0 ± 1.1 days; p = 0.0006). Under cyclosporine therapy, C57BL/6 heart survival was significantly longer for SCID/beige reconstituted with BD-S6 splenocytes (17.5 ± 6.4 days) than those reconstituted with BN-S6 splenocytes (6.2 ± 1.5 days; p < 0.0001). Conclusion: B cell depletion during allogeneic sensitization decreased memory T cells and recalls IFN-γ production and reduced second-set allograft rejection. {\circledC} 2009 Elsevier B.V. All rights reserved.",
keywords = "Allogeneic sensitization, B cell depletion, Memory T cells, CD4 antigen, CD8 antigen, cyclosporin, gamma interferon, Hermes antigen, L selectin, alloimmunity, animal cell, animal experiment, animal model, animal tissue, article, B lymphocyte, cardiac graft rejection, cell proliferation, cellular immunity, controlled study, cytokine production, female, graft survival, heart graft, memory T lymphocyte, mouse, nonhuman, priority journal, skin graft, spleen cell, survival time, T lymphocyte, Animals, Antigens, CD, B-Lymphocytes, Cell Proliferation, Graft Rejection, Immunologic Memory, Immunosuppression, Interferon-gamma, Lymphocyte Depletion, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, SCID, Skin Transplantation, T-Lymphocytes, Transplantation Chimera, Transplantation Conditioning",
author = "Meng-Kun Tsai and Hsiung-Fei Chien and Mei-Ching Tzeng and Po-Huang Lee",
note = "被引用次數:2 Export Date: 16 March 2016 CODEN: TRIME 通訊地址: Lee, P.-H.; Department of Surgery, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei, 100, Taiwan; 電子郵件: pohuang1115@ntu.edu.tw 化學物質/CAS: L selectin, 126880-86-2; cyclosporin, 79217-60-0; gamma interferon, 82115-62-6; Antigens, CD; Interferon-gamma, 82115-62-6 參考文獻: Genberg, H., Kumlien, G., Wennberg, L., Gerg, U., Tyden, G., ABO-incompatible kidney transplantation using antigen-specific immunoadsorption and rituximab: a 3-year follow-up (2008) Transplantation, 85, pp. 1745-1754; Faguer, S., Kamar, N., Guilbeaud-Grugier, C., Rituximab therapy for acute humoral rejection after kidney transplantation (2007) Transplantation, 83, pp. 1277-1280; Terasaki, P.I., Ozawa, M., Predicting kidney graft failure by HLA antibodies: a prospective trial (2004) Am J Transplant, 4, pp. 438-443; Billing, H., Reiger, S., Ovens, J., Successful treatment of chronic antibody-mediated rejection with IVIG and rituximab in pediatric renal transplantation recipients (2008) Transplantation, 86, pp. 1214-1221; Noorchashm, H., Reed, A.J., Rostami, S.Y., B cell-mediated antigen presentation is required for the pathogenesis of acute cardiac allograft rejection (2006) J Immunol, 177, pp. 7715-7722; Silveira, P.A., Johnson, E., Chapman, H.D., The preferential ability of B lymphocytes to act as diabetogenic APC in NOD mice depends on expression of self-antigen-specific immunoglobulin receptors (2002) Eur J Immunol, 32, pp. 3657-3666; Bouasziz, J., Yanaba, K., Venturi, G.M., Therapeutic B cell depletion impairs adaptive and autoreactive CD4+ T cell activation in mice (2007) PNAS, 104, pp. 20,878-20,883; Li, Y., Chen, F., Putt, M., B cell depletion with anti-CD79 mAbs ameliorates autoimmune disease in MRL/lpr mice (2008) J Immunol, 181, pp. 2961-2972; Chan OT, Hannum LG, Haberman AM, Madaio MP, Shlomchik MJ. A novel mouse with B cells but lacking serum antibody reveals an antibody-independent role for B cells in murine lupus. J Exp Med 1999; 189: 1639-1648. J Immunol. 1999; 163:3592-3596Chan, O.T., Madaio, M.P., Shlomchik, M.J., B cells are required for lupus nephritis in the polygenic, Fas-intact MRL model of systemic autoimmunity (1999) J Immunol, 163, pp. 3592-3596; Martin, F., Chan, A.C., B cell immunobiology in disease: evolving concepts from the clinic (2006) Ann Rev Immunol, 24, pp. 467-496; Epstein, M.M., Di Rossa, F., Jankovic, D., Sher, A., Matzinger, P., Successful T cell priming in B cell-deficient mice (1995) J Exp Med, 182, pp. 915-922; Hasan, M., Polic, B., Bralic, M., Jonjic, S., Rajewsky, K., Incomplete block of B cell development and immunoglobulin production in mice carrying the muMT mutation in the BALB/c background (2002) Eur J Immunol, 32, pp. 3463-3471; Protective immunity to HIV-1 in SCID/beige mice reconstituted with peripheral blood lymphocytes of exposed but uninfected individuals (1996) PNAS, 93, pp. 14,720-14,725; Topham, D.J., Tripp, R.A., Hamilton-Easton, A.M., Sarawar, S.R., Doherty, P.C., Quantitative analysis of the influenza virus-specific CD4+ T cell memory in absence of B cells and Ig (1996) J Immunol, 157, pp. 2947-2952; Tsai, M.K., Ho, H.N., Chien, H.F., Ou-Yang, P., Lee, C.J., Lee, P.H., The role of B7 ligands (CD80 and CD86) in CD152-mediated allograft tolerance: a crosscheck hypothesis (2004) Transplantation, 77, pp. 48-54; Haluszczak, C., Akue, A.D., Hamilton, S.E., The antigen-specific CD8+ T cell repertoire in unimmunized mice includes memory phenotype cells bearing markers of homeostatic expansion (2009) J Exp Med, 206, pp. 435-448; Tsai, M.K., Ho, H.N., Chien, H.F., Multiple negative feedbacks on CD152 expression in allograft tolerance (2005) Transplantation, 79, pp. 174-181; Macleod, M.K.L., McKee, A., Crawford, F., White, J., Kappler, J., Marrack, P., CD4 memory T cells divide poorly in response to antigen because of their cytokine profile (2008) PNAS, 105, pp. 14,521-14,526; Hu, C., Daniel, R., Du, W., Treatment with CD20-specific antibody prevents and reverses autoimmune diabetes in mice (2007) J Clin Invest, 117, pp. 3857-3867; Menard, L.C., Minns, L.A., Darche, S., B cells amplify IFN-γ production by T cells via a TNF-α-mediated mechanism (2007) J Immunol, 179, pp. 4857-4866; Nozaki, T., Rosenblum, Jm., Ishii, D., Tanabe, K., Fairchild, R.L., CD4 T cell-mediated rejection of cardiac allografts in B cell-deficient mice (2008) J Immunol, 181, pp. 5257-5263; Homann, D., Tishon, A., Berger, D.P., Evidence for an underlying CD4 helper and CD8 T-cell defect in B-cell-deficient mice: failure to clear persistent virus infection after adoptive immunotherapy with virus-specific memory cells from μMT/μMT mice (1998) J Virol, 72, pp. 9208-9216; Saout, C.L., Mennechet, S., Taylor, N., Hernandez, J., Memory-like CD8 and CD4+ T cells coerate to break eripheral tolerance under lymphopenic conditions (2008) PNAS, 105, pp. 19,414-19,419; Bingaman, A.W., Farber, D.L., Memory T cells in transplantation: generation, function, and potential role in rejection (2004) Am J Transplant, 4, pp. 846-852; Lee, P.C., Terasaki, P.I., Takemoto, S.K., All chronic rejection failures of kidney transplant were preceded by the development of HLA antibodies (2002) Transplantation, 74, pp. 1192-1194; Gupta, M., Satyaraj, E., Durdik, J.M., Rath, S., Bal, V., Differential regulation of T cells activation for primary versus secondary proliferative responses (1997) J Immunol, 158, pp. 4113-4121; Trzonkowski, P., Zilvetti, M., Friend, P., Wood, K.J., Recipient memory-like lymphocytes remain unresponsive to graft antigens after CAMPATH-1H induction with reduced maintenance immunosuppression (2006) Transplantation, 82, pp. 1342-1351; Bestard, O., Nickel P Cruzado, J.M., Circulating alloreactive T cells correlated with graft function in longstanding renal transplant recipients (2008) J Am Soc Nephrol, 19, pp. 1419-1429; Becker, Y.T., Becker, B.N., Pirsch, J.D., Sollinger, H.W., Rituximab as treatment for refractory kidney transplant rejection (2004) Am J Transplant, 4, pp. 996-1001",
year = "2009",
doi = "10.1016/j.trim.2009.06.006",
language = "English",
volume = "21",
pages = "215--220",
journal = "Transplant Immunology",
issn = "0966-3274",
publisher = "Elsevier",
number = "4",

}

TY - JOUR

T1 - Effects of B cell depletion on T cell allogeneic Immune responses: A strategy to reduce allogeneic sensitization

AU - Tsai, Meng-Kun

AU - Chien, Hsiung-Fei

AU - Tzeng, Mei-Ching

AU - Lee, Po-Huang

N1 - 被引用次數:2 Export Date: 16 March 2016 CODEN: TRIME 通訊地址: Lee, P.-H.; Department of Surgery, National Taiwan University Hospital, No. 7, Chung-Shan South Road, Taipei, 100, Taiwan; 電子郵件: pohuang1115@ntu.edu.tw 化學物質/CAS: L selectin, 126880-86-2; cyclosporin, 79217-60-0; gamma interferon, 82115-62-6; Antigens, CD; Interferon-gamma, 82115-62-6 參考文獻: Genberg, H., Kumlien, G., Wennberg, L., Gerg, U., Tyden, G., ABO-incompatible kidney transplantation using antigen-specific immunoadsorption and rituximab: a 3-year follow-up (2008) Transplantation, 85, pp. 1745-1754; Faguer, S., Kamar, N., Guilbeaud-Grugier, C., Rituximab therapy for acute humoral rejection after kidney transplantation (2007) Transplantation, 83, pp. 1277-1280; Terasaki, P.I., Ozawa, M., Predicting kidney graft failure by HLA antibodies: a prospective trial (2004) Am J Transplant, 4, pp. 438-443; Billing, H., Reiger, S., Ovens, J., Successful treatment of chronic antibody-mediated rejection with IVIG and rituximab in pediatric renal transplantation recipients (2008) Transplantation, 86, pp. 1214-1221; Noorchashm, H., Reed, A.J., Rostami, S.Y., B cell-mediated antigen presentation is required for the pathogenesis of acute cardiac allograft rejection (2006) J Immunol, 177, pp. 7715-7722; Silveira, P.A., Johnson, E., Chapman, H.D., The preferential ability of B lymphocytes to act as diabetogenic APC in NOD mice depends on expression of self-antigen-specific immunoglobulin receptors (2002) Eur J Immunol, 32, pp. 3657-3666; Bouasziz, J., Yanaba, K., Venturi, G.M., Therapeutic B cell depletion impairs adaptive and autoreactive CD4+ T cell activation in mice (2007) PNAS, 104, pp. 20,878-20,883; Li, Y., Chen, F., Putt, M., B cell depletion with anti-CD79 mAbs ameliorates autoimmune disease in MRL/lpr mice (2008) J Immunol, 181, pp. 2961-2972; Chan OT, Hannum LG, Haberman AM, Madaio MP, Shlomchik MJ. A novel mouse with B cells but lacking serum antibody reveals an antibody-independent role for B cells in murine lupus. J Exp Med 1999; 189: 1639-1648. J Immunol. 1999; 163:3592-3596Chan, O.T., Madaio, M.P., Shlomchik, M.J., B cells are required for lupus nephritis in the polygenic, Fas-intact MRL model of systemic autoimmunity (1999) J Immunol, 163, pp. 3592-3596; Martin, F., Chan, A.C., B cell immunobiology in disease: evolving concepts from the clinic (2006) Ann Rev Immunol, 24, pp. 467-496; Epstein, M.M., Di Rossa, F., Jankovic, D., Sher, A., Matzinger, P., Successful T cell priming in B cell-deficient mice (1995) J Exp Med, 182, pp. 915-922; Hasan, M., Polic, B., Bralic, M., Jonjic, S., Rajewsky, K., Incomplete block of B cell development and immunoglobulin production in mice carrying the muMT mutation in the BALB/c background (2002) Eur J Immunol, 32, pp. 3463-3471; Protective immunity to HIV-1 in SCID/beige mice reconstituted with peripheral blood lymphocytes of exposed but uninfected individuals (1996) PNAS, 93, pp. 14,720-14,725; Topham, D.J., Tripp, R.A., Hamilton-Easton, A.M., Sarawar, S.R., Doherty, P.C., Quantitative analysis of the influenza virus-specific CD4+ T cell memory in absence of B cells and Ig (1996) J Immunol, 157, pp. 2947-2952; Tsai, M.K., Ho, H.N., Chien, H.F., Ou-Yang, P., Lee, C.J., Lee, P.H., The role of B7 ligands (CD80 and CD86) in CD152-mediated allograft tolerance: a crosscheck hypothesis (2004) Transplantation, 77, pp. 48-54; Haluszczak, C., Akue, A.D., Hamilton, S.E., The antigen-specific CD8+ T cell repertoire in unimmunized mice includes memory phenotype cells bearing markers of homeostatic expansion (2009) J Exp Med, 206, pp. 435-448; Tsai, M.K., Ho, H.N., Chien, H.F., Multiple negative feedbacks on CD152 expression in allograft tolerance (2005) Transplantation, 79, pp. 174-181; Macleod, M.K.L., McKee, A., Crawford, F., White, J., Kappler, J., Marrack, P., CD4 memory T cells divide poorly in response to antigen because of their cytokine profile (2008) PNAS, 105, pp. 14,521-14,526; Hu, C., Daniel, R., Du, W., Treatment with CD20-specific antibody prevents and reverses autoimmune diabetes in mice (2007) J Clin Invest, 117, pp. 3857-3867; Menard, L.C., Minns, L.A., Darche, S., B cells amplify IFN-γ production by T cells via a TNF-α-mediated mechanism (2007) J Immunol, 179, pp. 4857-4866; Nozaki, T., Rosenblum, Jm., Ishii, D., Tanabe, K., Fairchild, R.L., CD4 T cell-mediated rejection of cardiac allografts in B cell-deficient mice (2008) J Immunol, 181, pp. 5257-5263; Homann, D., Tishon, A., Berger, D.P., Evidence for an underlying CD4 helper and CD8 T-cell defect in B-cell-deficient mice: failure to clear persistent virus infection after adoptive immunotherapy with virus-specific memory cells from μMT/μMT mice (1998) J Virol, 72, pp. 9208-9216; Saout, C.L., Mennechet, S., Taylor, N., Hernandez, J., Memory-like CD8 and CD4+ T cells coerate to break eripheral tolerance under lymphopenic conditions (2008) PNAS, 105, pp. 19,414-19,419; Bingaman, A.W., Farber, D.L., Memory T cells in transplantation: generation, function, and potential role in rejection (2004) Am J Transplant, 4, pp. 846-852; Lee, P.C., Terasaki, P.I., Takemoto, S.K., All chronic rejection failures of kidney transplant were preceded by the development of HLA antibodies (2002) Transplantation, 74, pp. 1192-1194; Gupta, M., Satyaraj, E., Durdik, J.M., Rath, S., Bal, V., Differential regulation of T cells activation for primary versus secondary proliferative responses (1997) J Immunol, 158, pp. 4113-4121; Trzonkowski, P., Zilvetti, M., Friend, P., Wood, K.J., Recipient memory-like lymphocytes remain unresponsive to graft antigens after CAMPATH-1H induction with reduced maintenance immunosuppression (2006) Transplantation, 82, pp. 1342-1351; Bestard, O., Nickel P Cruzado, J.M., Circulating alloreactive T cells correlated with graft function in longstanding renal transplant recipients (2008) J Am Soc Nephrol, 19, pp. 1419-1429; Becker, Y.T., Becker, B.N., Pirsch, J.D., Sollinger, H.W., Rituximab as treatment for refractory kidney transplant rejection (2004) Am J Transplant, 4, pp. 996-1001

PY - 2009

Y1 - 2009

N2 - Background: B cell depletion has been employed to treat antibody-mediated organ transplantation rejection, although the effects on cellular immune responses have not been extensively investigated. Methods: A model of B cell depletion used SCID/beige mice reconstituted with BALB/c splenocytes either depleted of B cells (BD) or not (BN). BD and B/N mice received C57BL/6 skin grafts and were sacrificed after 6 weeks (BD-S6 and BN-S6). Results: Recall proliferative responses of BD-S6 splenocytes to C57BL/6 were significantly reduced compared to BN-S6, and central memory T cells' proportions (CD4+CD44+CD62L+ or CD8+CD44+CD62L+) were significantly decreased in BD-S6 spleens. Recall IFN-γ production by BD-S6 splenocytes was significantly reduced compared to BN-S6 splenocytes (p = 0.0028). Survival times of C57BL/6 heart grafts were significantly longer in SCID/beige mice reconstituted with BD-S6 splenocytes (8.5 ± 1.1 days) than for SCID/beige reconstituted with BN-S6 splenocytes (6.0 ± 1.1 days; p = 0.0006). Under cyclosporine therapy, C57BL/6 heart survival was significantly longer for SCID/beige reconstituted with BD-S6 splenocytes (17.5 ± 6.4 days) than those reconstituted with BN-S6 splenocytes (6.2 ± 1.5 days; p < 0.0001). Conclusion: B cell depletion during allogeneic sensitization decreased memory T cells and recalls IFN-γ production and reduced second-set allograft rejection. © 2009 Elsevier B.V. All rights reserved.

AB - Background: B cell depletion has been employed to treat antibody-mediated organ transplantation rejection, although the effects on cellular immune responses have not been extensively investigated. Methods: A model of B cell depletion used SCID/beige mice reconstituted with BALB/c splenocytes either depleted of B cells (BD) or not (BN). BD and B/N mice received C57BL/6 skin grafts and were sacrificed after 6 weeks (BD-S6 and BN-S6). Results: Recall proliferative responses of BD-S6 splenocytes to C57BL/6 were significantly reduced compared to BN-S6, and central memory T cells' proportions (CD4+CD44+CD62L+ or CD8+CD44+CD62L+) were significantly decreased in BD-S6 spleens. Recall IFN-γ production by BD-S6 splenocytes was significantly reduced compared to BN-S6 splenocytes (p = 0.0028). Survival times of C57BL/6 heart grafts were significantly longer in SCID/beige mice reconstituted with BD-S6 splenocytes (8.5 ± 1.1 days) than for SCID/beige reconstituted with BN-S6 splenocytes (6.0 ± 1.1 days; p = 0.0006). Under cyclosporine therapy, C57BL/6 heart survival was significantly longer for SCID/beige reconstituted with BD-S6 splenocytes (17.5 ± 6.4 days) than those reconstituted with BN-S6 splenocytes (6.2 ± 1.5 days; p < 0.0001). Conclusion: B cell depletion during allogeneic sensitization decreased memory T cells and recalls IFN-γ production and reduced second-set allograft rejection. © 2009 Elsevier B.V. All rights reserved.

KW - Allogeneic sensitization

KW - B cell depletion

KW - Memory T cells

KW - CD4 antigen

KW - CD8 antigen

KW - cyclosporin

KW - gamma interferon

KW - Hermes antigen

KW - L selectin

KW - alloimmunity

KW - animal cell

KW - animal experiment

KW - animal model

KW - animal tissue

KW - article

KW - B lymphocyte

KW - cardiac graft rejection

KW - cell proliferation

KW - cellular immunity

KW - controlled study

KW - cytokine production

KW - female

KW - graft survival

KW - heart graft

KW - memory T lymphocyte

KW - mouse

KW - nonhuman

KW - priority journal

KW - skin graft

KW - spleen cell

KW - survival time

KW - T lymphocyte

KW - Animals

KW - Antigens, CD

KW - B-Lymphocytes

KW - Cell Proliferation

KW - Graft Rejection

KW - Immunologic Memory

KW - Immunosuppression

KW - Interferon-gamma

KW - Lymphocyte Depletion

KW - Mice

KW - Mice, Inbred BALB C

KW - Mice, Inbred C57BL

KW - Mice, SCID

KW - Skin Transplantation

KW - T-Lymphocytes

KW - Transplantation Chimera

KW - Transplantation Conditioning

U2 - 10.1016/j.trim.2009.06.006

DO - 10.1016/j.trim.2009.06.006

M3 - Article

VL - 21

SP - 215

EP - 220

JO - Transplant Immunology

JF - Transplant Immunology

SN - 0966-3274

IS - 4

ER -