Effects of B cell depletion on T cell allogeneic Immune responses: A strategy to reduce allogeneic sensitization

Meng-Kun Tsai, Hsiung-Fei Chien, Mei-Ching Tzeng, Po-Huang Lee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: B cell depletion has been employed to treat antibody-mediated organ transplantation rejection, although the effects on cellular immune responses have not been extensively investigated. Methods: A model of B cell depletion used SCID/beige mice reconstituted with BALB/c splenocytes either depleted of B cells (BD) or not (BN). BD and B/N mice received C57BL/6 skin grafts and were sacrificed after 6 weeks (BD-S6 and BN-S6). Results: Recall proliferative responses of BD-S6 splenocytes to C57BL/6 were significantly reduced compared to BN-S6, and central memory T cells' proportions (CD4+CD44+CD62L+ or CD8+CD44+CD62L+) were significantly decreased in BD-S6 spleens. Recall IFN-γ production by BD-S6 splenocytes was significantly reduced compared to BN-S6 splenocytes (p = 0.0028). Survival times of C57BL/6 heart grafts were significantly longer in SCID/beige mice reconstituted with BD-S6 splenocytes (8.5 ± 1.1 days) than for SCID/beige reconstituted with BN-S6 splenocytes (6.0 ± 1.1 days; p = 0.0006). Under cyclosporine therapy, C57BL/6 heart survival was significantly longer for SCID/beige reconstituted with BD-S6 splenocytes (17.5 ± 6.4 days) than those reconstituted with BN-S6 splenocytes (6.2 ± 1.5 days; p < 0.0001). Conclusion: B cell depletion during allogeneic sensitization decreased memory T cells and recalls IFN-γ production and reduced second-set allograft rejection. © 2009 Elsevier B.V. All rights reserved.
Original languageEnglish
Pages (from-to)215-220
Number of pages6
JournalTransplant Immunology
Volume21
Issue number4
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Allogeneic sensitization
  • B cell depletion
  • Memory T cells
  • CD4 antigen
  • CD8 antigen
  • cyclosporin
  • gamma interferon
  • Hermes antigen
  • L selectin
  • alloimmunity
  • animal cell
  • animal experiment
  • animal model
  • animal tissue
  • article
  • B lymphocyte
  • cardiac graft rejection
  • cell proliferation
  • cellular immunity
  • controlled study
  • cytokine production
  • female
  • graft survival
  • heart graft
  • memory T lymphocyte
  • mouse
  • nonhuman
  • priority journal
  • skin graft
  • spleen cell
  • survival time
  • T lymphocyte
  • Animals
  • Antigens, CD
  • B-Lymphocytes
  • Cell Proliferation
  • Graft Rejection
  • Immunologic Memory
  • Immunosuppression
  • Interferon-gamma
  • Lymphocyte Depletion
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, SCID
  • Skin Transplantation
  • T-Lymphocytes
  • Transplantation Chimera
  • Transplantation Conditioning

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