Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5

Yasumasa Shirouzu, Goichi Yanai, Kai Chiang Yang, Shoichiro Sumi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.

Original languageEnglish
Pages (from-to)171-186
Number of pages16
JournalCellular Reprogramming
Volume18
Issue number3
DOIs
Publication statusPublished - Jun 1 2016
Externally publishedYes

Fingerprint

Fibroblast Growth Factor 5
Embryoid Bodies
Activins
Endoderm
Germ Layers
Tretinoin
Gelatin
Embryonic Development
Cell Differentiation

ASJC Scopus subject areas

  • Biotechnology
  • Developmental Biology
  • Cell Biology

Cite this

Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5. / Shirouzu, Yasumasa; Yanai, Goichi; Yang, Kai Chiang; Sumi, Shoichiro.

In: Cellular Reprogramming, Vol. 18, No. 3, 01.06.2016, p. 171-186.

Research output: Contribution to journalArticle

Shirouzu, Yasumasa ; Yanai, Goichi ; Yang, Kai Chiang ; Sumi, Shoichiro. / Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5. In: Cellular Reprogramming. 2016 ; Vol. 18, No. 3. pp. 171-186.
@article{87759ee2a3d94fe4a014bda1f73209ed,
title = "Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5",
abstract = "Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.",
author = "Yasumasa Shirouzu and Goichi Yanai and Yang, {Kai Chiang} and Shoichiro Sumi",
year = "2016",
month = "6",
day = "1",
doi = "10.1089/cell.2015.0074",
language = "English",
volume = "18",
pages = "171--186",
journal = "Cellular Reprogramming",
issn = "2152-4971",
publisher = "Mary Ann Liebert Inc.",
number = "3",

}

TY - JOUR

T1 - Effects of Activin in Embryoid Bodies Expressing Fibroblast Growth Factor 5

AU - Shirouzu, Yasumasa

AU - Yanai, Goichi

AU - Yang, Kai Chiang

AU - Sumi, Shoichiro

PY - 2016/6/1

Y1 - 2016/6/1

N2 - Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.

AB - Nodal/activin signaling is indispensable for embryonic development. We examined what activin does to the embryoid bodies (EBs) produced from mouse embryonic stem cells (mESCs) expressing an epiblast marker. The EBs were produced by culturing mESCs by the hanging drop method for 24 hours. The resulting EBs were transferred onto gelatin-coated dishes and allowed to further differentiate. The 24-hour EBs showed a stronger expression of fibroblast growth factor (FGF)5 and Brachyury (specific to the epiblast) in comparison with mESCs. Treating the transferred EBs with activin A maintained transcript levels of FGF5 and Oct4, while inhibiting definitive endoderm differentiation. The activin A treatment reversed the endoderm differentiation induced by retinoic acid (RA), while the inhibition of nodal/activin signaling promoted RA-induced endoderm differentiation. Inhibition of nodal/activin signaling in EBs, including epiblast-like cells, promotes differentiation into the endoderm, facilitating the transition from the pluripotent state to specification of the endoderm.

UR - http://www.scopus.com/inward/record.url?scp=84973387011&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973387011&partnerID=8YFLogxK

U2 - 10.1089/cell.2015.0074

DO - 10.1089/cell.2015.0074

M3 - Article

C2 - 27253628

AN - SCOPUS:84973387011

VL - 18

SP - 171

EP - 186

JO - Cellular Reprogramming

JF - Cellular Reprogramming

SN - 2152-4971

IS - 3

ER -