Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture

S. R. Stapleton, G. J. Stevens, J. F. Teel, K. B. Rank, E. A. Berg, J. Y. Wu, L. C. Ginsberg, R. F. Kletzien

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Ethanol has been shown to induce the activity of glucose-6-phosphate dehydrogenase (G6PDH). To clarify the mechanism behind this induction, we examined the role of acetaldehyde (AA), the first product of ethanol metabolism. In primary adult rat hepatocytes maintained in chemically defined medium, we examined the effect of AA on G6PDH activity, mRNA levels and lipid synthesis. We observe a 40% increase in G6PDH activity and a similar increase in mRNA levels, following exposure to 100 μM AA. The increase in activity was found to be maximal at 24 h while mRNA levels increased over controls as early as 3 h. The induction in G6PDH by AA was found to occur at lower concentrations and earlier time points than those reported using ethanol. The role of insulin, a known inducer of G6PDH activity was studied alone and in combination with AA on both G6PDH activity and mRNA levels as well as lipid biosynthesis. Insulin (300 ng/ml) was found to increase G6PDH activity, mRNA levels and [14C]-acetate incorporation into lipid. It was also shown to have an additive effect with AA on G6PDH activity, suggesting their actions are mediated via different mechanistic pathways. No change in [14C]-acetate incorporation into lipid, however, was observed with acetaldehyde alone.

Original languageEnglish
Pages (from-to)971-976
Number of pages6
JournalBiochimie
Volume75
Issue number11
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Acetaldehyde
Glucosephosphate Dehydrogenase
Rats
Hepatocytes
Messenger RNA
Lipids
Ethanol
Acetates
Insulin
Biosynthesis
Metabolism

Keywords

  • acetaldehyde
  • glucose-6-phosphate dehydrogenase
  • primary rat hepatocytes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture. / Stapleton, S. R.; Stevens, G. J.; Teel, J. F.; Rank, K. B.; Berg, E. A.; Wu, J. Y.; Ginsberg, L. C.; Kletzien, R. F.

In: Biochimie, Vol. 75, No. 11, 1993, p. 971-976.

Research output: Contribution to journalArticle

Stapleton, SR, Stevens, GJ, Teel, JF, Rank, KB, Berg, EA, Wu, JY, Ginsberg, LC & Kletzien, RF 1993, 'Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture', Biochimie, vol. 75, no. 11, pp. 971-976. https://doi.org/10.1016/0300-9084(93)90147-K
Stapleton, S. R. ; Stevens, G. J. ; Teel, J. F. ; Rank, K. B. ; Berg, E. A. ; Wu, J. Y. ; Ginsberg, L. C. ; Kletzien, R. F. / Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture. In: Biochimie. 1993 ; Vol. 75, No. 11. pp. 971-976.
@article{3ec5076376ba4537a0493959a5cf31b4,
title = "Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture",
abstract = "Ethanol has been shown to induce the activity of glucose-6-phosphate dehydrogenase (G6PDH). To clarify the mechanism behind this induction, we examined the role of acetaldehyde (AA), the first product of ethanol metabolism. In primary adult rat hepatocytes maintained in chemically defined medium, we examined the effect of AA on G6PDH activity, mRNA levels and lipid synthesis. We observe a 40{\%} increase in G6PDH activity and a similar increase in mRNA levels, following exposure to 100 μM AA. The increase in activity was found to be maximal at 24 h while mRNA levels increased over controls as early as 3 h. The induction in G6PDH by AA was found to occur at lower concentrations and earlier time points than those reported using ethanol. The role of insulin, a known inducer of G6PDH activity was studied alone and in combination with AA on both G6PDH activity and mRNA levels as well as lipid biosynthesis. Insulin (300 ng/ml) was found to increase G6PDH activity, mRNA levels and [14C]-acetate incorporation into lipid. It was also shown to have an additive effect with AA on G6PDH activity, suggesting their actions are mediated via different mechanistic pathways. No change in [14C]-acetate incorporation into lipid, however, was observed with acetaldehyde alone.",
keywords = "acetaldehyde, glucose-6-phosphate dehydrogenase, primary rat hepatocytes",
author = "Stapleton, {S. R.} and Stevens, {G. J.} and Teel, {J. F.} and Rank, {K. B.} and Berg, {E. A.} and Wu, {J. Y.} and Ginsberg, {L. C.} and Kletzien, {R. F.}",
year = "1993",
doi = "10.1016/0300-9084(93)90147-K",
language = "English",
volume = "75",
pages = "971--976",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier",
number = "11",

}

TY - JOUR

T1 - Effects of acetaldehyde on glucose-6-phosphate dehydrogenase activity and mRNA levels in primary rat hepatocytes in culture

AU - Stapleton, S. R.

AU - Stevens, G. J.

AU - Teel, J. F.

AU - Rank, K. B.

AU - Berg, E. A.

AU - Wu, J. Y.

AU - Ginsberg, L. C.

AU - Kletzien, R. F.

PY - 1993

Y1 - 1993

N2 - Ethanol has been shown to induce the activity of glucose-6-phosphate dehydrogenase (G6PDH). To clarify the mechanism behind this induction, we examined the role of acetaldehyde (AA), the first product of ethanol metabolism. In primary adult rat hepatocytes maintained in chemically defined medium, we examined the effect of AA on G6PDH activity, mRNA levels and lipid synthesis. We observe a 40% increase in G6PDH activity and a similar increase in mRNA levels, following exposure to 100 μM AA. The increase in activity was found to be maximal at 24 h while mRNA levels increased over controls as early as 3 h. The induction in G6PDH by AA was found to occur at lower concentrations and earlier time points than those reported using ethanol. The role of insulin, a known inducer of G6PDH activity was studied alone and in combination with AA on both G6PDH activity and mRNA levels as well as lipid biosynthesis. Insulin (300 ng/ml) was found to increase G6PDH activity, mRNA levels and [14C]-acetate incorporation into lipid. It was also shown to have an additive effect with AA on G6PDH activity, suggesting their actions are mediated via different mechanistic pathways. No change in [14C]-acetate incorporation into lipid, however, was observed with acetaldehyde alone.

AB - Ethanol has been shown to induce the activity of glucose-6-phosphate dehydrogenase (G6PDH). To clarify the mechanism behind this induction, we examined the role of acetaldehyde (AA), the first product of ethanol metabolism. In primary adult rat hepatocytes maintained in chemically defined medium, we examined the effect of AA on G6PDH activity, mRNA levels and lipid synthesis. We observe a 40% increase in G6PDH activity and a similar increase in mRNA levels, following exposure to 100 μM AA. The increase in activity was found to be maximal at 24 h while mRNA levels increased over controls as early as 3 h. The induction in G6PDH by AA was found to occur at lower concentrations and earlier time points than those reported using ethanol. The role of insulin, a known inducer of G6PDH activity was studied alone and in combination with AA on both G6PDH activity and mRNA levels as well as lipid biosynthesis. Insulin (300 ng/ml) was found to increase G6PDH activity, mRNA levels and [14C]-acetate incorporation into lipid. It was also shown to have an additive effect with AA on G6PDH activity, suggesting their actions are mediated via different mechanistic pathways. No change in [14C]-acetate incorporation into lipid, however, was observed with acetaldehyde alone.

KW - acetaldehyde

KW - glucose-6-phosphate dehydrogenase

KW - primary rat hepatocytes

UR - http://www.scopus.com/inward/record.url?scp=0027717402&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027717402&partnerID=8YFLogxK

U2 - 10.1016/0300-9084(93)90147-K

DO - 10.1016/0300-9084(93)90147-K

M3 - Article

VL - 75

SP - 971

EP - 976

JO - Biochimie

JF - Biochimie

SN - 0300-9084

IS - 11

ER -