Abstract

Introduction: The objective of this study was to investigate the antibacterial, biocompatibility, and mechanical properties of mineral trioxide aggregate (MTA) set using a calcium lactate gluconate (CLG) solution. Methods: ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Tulsa, OK) was used as the control group; MTA-like cement was prepared by mixing Portland cement/bismuth oxide/calcium sulfate (75/20/5) as the experiment group. A solution of 23.1 wt% CLG was used as a hydration accelerant and was compared with deionized water (DDW). Changes in pH values, antibacterial properties, in vitro cell viability, and diametral tensile strength (DTS) of the hydrated cements were assessed. Results: Like WMTA, pH values for the MTA-like cement set using DDW and the CLG solution showed minor but statistically significant differences (P <.05). The antibacterial effects of hydrated specimens set by DDW and CLG against Streptococcus mutans assessed with an in vitro tube dilution test showed a significant difference in the early hydration time but no significant difference after 60 minutes (P > .05). A mouse osteoblastic cell (MC3T3-E1)-based MTT assay revealed that WMTA set using CLG had significantly higher cell viability than that set using DDW (P <.05). The DTS test for hydrated MTA-like cement with different liquids showed a significant difference on day 1 but no statistical difference on day 21. Conclusions: The results suggest that using a CLG solution as the hydration accelerant may enhance the biocompatibility but not compromise WMTA's antibacterial and mechanical properties.

Original languageEnglish
Pages (from-to)851-855
Number of pages5
JournalJournal of Endodontics
Volume37
Issue number6
DOIs
Publication statusPublished - Jun 2011

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Calcium Gluconate
Pemetrexed
Tensile Strength
Water
Cell Survival
Calcium Sulfate
Tooth
calcium lactate
mineral trioxide aggregate
Control Groups

Keywords

  • Calcium lactate gluconate
  • white mineral trioxide aggregate

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

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title = "Effects of a novel hydration accelerant on the biological and mechanical properties of white mineral trioxide aggregate",
abstract = "Introduction: The objective of this study was to investigate the antibacterial, biocompatibility, and mechanical properties of mineral trioxide aggregate (MTA) set using a calcium lactate gluconate (CLG) solution. Methods: ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Tulsa, OK) was used as the control group; MTA-like cement was prepared by mixing Portland cement/bismuth oxide/calcium sulfate (75/20/5) as the experiment group. A solution of 23.1 wt{\%} CLG was used as a hydration accelerant and was compared with deionized water (DDW). Changes in pH values, antibacterial properties, in vitro cell viability, and diametral tensile strength (DTS) of the hydrated cements were assessed. Results: Like WMTA, pH values for the MTA-like cement set using DDW and the CLG solution showed minor but statistically significant differences (P <.05). The antibacterial effects of hydrated specimens set by DDW and CLG against Streptococcus mutans assessed with an in vitro tube dilution test showed a significant difference in the early hydration time but no significant difference after 60 minutes (P > .05). A mouse osteoblastic cell (MC3T3-E1)-based MTT assay revealed that WMTA set using CLG had significantly higher cell viability than that set using DDW (P <.05). The DTS test for hydrated MTA-like cement with different liquids showed a significant difference on day 1 but no statistical difference on day 21. Conclusions: The results suggest that using a CLG solution as the hydration accelerant may enhance the biocompatibility but not compromise WMTA's antibacterial and mechanical properties.",
keywords = "Calcium lactate gluconate, white mineral trioxide aggregate",
author = "Ji, {Dian Yu} and Wu, {Hong Da} and Hsieh, {Sung Chih} and Teng, {Nai Chia} and Chen, {Chien Chung} and Ke, {En Sheng} and Lin, {Yu Chen} and Lee, {Sheng Yang} and Yang, {Jen Chang}",
year = "2011",
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language = "English",
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TY - JOUR

T1 - Effects of a novel hydration accelerant on the biological and mechanical properties of white mineral trioxide aggregate

AU - Ji, Dian Yu

AU - Wu, Hong Da

AU - Hsieh, Sung Chih

AU - Teng, Nai Chia

AU - Chen, Chien Chung

AU - Ke, En Sheng

AU - Lin, Yu Chen

AU - Lee, Sheng Yang

AU - Yang, Jen Chang

PY - 2011/6

Y1 - 2011/6

N2 - Introduction: The objective of this study was to investigate the antibacterial, biocompatibility, and mechanical properties of mineral trioxide aggregate (MTA) set using a calcium lactate gluconate (CLG) solution. Methods: ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Tulsa, OK) was used as the control group; MTA-like cement was prepared by mixing Portland cement/bismuth oxide/calcium sulfate (75/20/5) as the experiment group. A solution of 23.1 wt% CLG was used as a hydration accelerant and was compared with deionized water (DDW). Changes in pH values, antibacterial properties, in vitro cell viability, and diametral tensile strength (DTS) of the hydrated cements were assessed. Results: Like WMTA, pH values for the MTA-like cement set using DDW and the CLG solution showed minor but statistically significant differences (P <.05). The antibacterial effects of hydrated specimens set by DDW and CLG against Streptococcus mutans assessed with an in vitro tube dilution test showed a significant difference in the early hydration time but no significant difference after 60 minutes (P > .05). A mouse osteoblastic cell (MC3T3-E1)-based MTT assay revealed that WMTA set using CLG had significantly higher cell viability than that set using DDW (P <.05). The DTS test for hydrated MTA-like cement with different liquids showed a significant difference on day 1 but no statistical difference on day 21. Conclusions: The results suggest that using a CLG solution as the hydration accelerant may enhance the biocompatibility but not compromise WMTA's antibacterial and mechanical properties.

AB - Introduction: The objective of this study was to investigate the antibacterial, biocompatibility, and mechanical properties of mineral trioxide aggregate (MTA) set using a calcium lactate gluconate (CLG) solution. Methods: ProRoot white MTA (WMTA) (Dentsply Tulsa Dental, Tulsa, OK) was used as the control group; MTA-like cement was prepared by mixing Portland cement/bismuth oxide/calcium sulfate (75/20/5) as the experiment group. A solution of 23.1 wt% CLG was used as a hydration accelerant and was compared with deionized water (DDW). Changes in pH values, antibacterial properties, in vitro cell viability, and diametral tensile strength (DTS) of the hydrated cements were assessed. Results: Like WMTA, pH values for the MTA-like cement set using DDW and the CLG solution showed minor but statistically significant differences (P <.05). The antibacterial effects of hydrated specimens set by DDW and CLG against Streptococcus mutans assessed with an in vitro tube dilution test showed a significant difference in the early hydration time but no significant difference after 60 minutes (P > .05). A mouse osteoblastic cell (MC3T3-E1)-based MTT assay revealed that WMTA set using CLG had significantly higher cell viability than that set using DDW (P <.05). The DTS test for hydrated MTA-like cement with different liquids showed a significant difference on day 1 but no statistical difference on day 21. Conclusions: The results suggest that using a CLG solution as the hydration accelerant may enhance the biocompatibility but not compromise WMTA's antibacterial and mechanical properties.

KW - Calcium lactate gluconate

KW - white mineral trioxide aggregate

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U2 - 10.1016/j.joen.2011.03.015

DO - 10.1016/j.joen.2011.03.015

M3 - Article

VL - 37

SP - 851

EP - 855

JO - Journal of Endodontics

JF - Journal of Endodontics

SN - 0099-2399

IS - 6

ER -