Effects of a Chinese herbal health formula, "Gan-Lu-Yin", on angiogenesis

Chun Hsu Pan, I. Chun Hsieh, Fon Chang Liu, Wen Tsong Hsieh, Ming Jyh Sheu, Ayano Koizumi, Chieh Hsi Wu

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

According to the known effects of each ingredient, Gan-Lu-Yin (GLY), a traditional Chinese herbal formula, has the potential to be an antiangiogenic agent. The purpose of this study was to explore the putative effect of GLY on antiangiogenesis. An ethanol extract of GLY was tested on chicken chorioallantoic membrane (CAM) and human umbilical vein endothelial cells (HUVEC) to evaluate the effects of GLY extract on cell proliferation, migration, and tube formation. The results showed that treatment with 1.0 mg/mL of GLY extract could markedly reduce cell migration and in vitro tube formation of HUVEC, and 1.5 mg/mL of GLY extract was sufficient to inhibit proliferation of HUVEC. The expression level of vascular endothelial growth factor (VEGF) of HUVEC was significantly decreased by 1.5 and 2.0 mg/mL of GLY extract. In chicken CAM assay, all tested concentrations of GLY extract were found to reduce the capillary mesh on the CAM of fertilized eggs. The inhibitory effects of GLY extract (1 mg/mL) were also found on tumor cell-induced HUVEC proliferation and tube formation. These observations suggested that GLY extract has an inhibitory effect on angiogenesis, which in turn may prevent tumor growth, and its mechanism might be partially associated with blocking VEGF protein expression of HUVEC.

Original languageEnglish
Pages (from-to)7685-7692
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number13
DOIs
Publication statusPublished - Jul 14 2010
Externally publishedYes

Fingerprint

Endothelial cells
Human Umbilical Vein Endothelial Cells
angiogenesis
Health
Chorioallantoic Membrane
extracts
chorioallantoic membrane
Cell proliferation
Membranes
Vascular Endothelial Growth Factor A
Cell Movement
vascular endothelial growth factors
Tumors
Chickens
Cell Proliferation
cell proliferation
Angiogenesis Inhibitors
Zygote
chickens
Assays

Keywords

  • Anglogenesis
  • Gan-Lu-Yin
  • Vascular endothelial cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)
  • Medicine(all)

Cite this

Effects of a Chinese herbal health formula, "Gan-Lu-Yin", on angiogenesis. / Pan, Chun Hsu; Hsieh, I. Chun; Liu, Fon Chang; Hsieh, Wen Tsong; Sheu, Ming Jyh; Koizumi, Ayano; Wu, Chieh Hsi.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 13, 14.07.2010, p. 7685-7692.

Research output: Contribution to journalArticle

Pan, Chun Hsu ; Hsieh, I. Chun ; Liu, Fon Chang ; Hsieh, Wen Tsong ; Sheu, Ming Jyh ; Koizumi, Ayano ; Wu, Chieh Hsi. / Effects of a Chinese herbal health formula, "Gan-Lu-Yin", on angiogenesis. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 13. pp. 7685-7692.
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