Effects of 6-Gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells

Ching Chiung Wang, Lih Geeng Chen, Lain Tze Lee, Ling-Ling Yang

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

6-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger. In this paper, the antioxidative effects of 6-gingerol were detected by DPPH and DCFH assays and, as predicted, 6-gingerol as an antioxidant was shown to protect HL-60 cells from oxidative stress. Moreover, it induced cell death in promyelocytic leukemia HL-60 cells, caused DNA fragmentation and inhibited Bcl-2 expression in HL-60 cells. These results suggested that the inhibition of Bcl-2 expression in HL-60 cells might account for the mechanism of 6-gingerol-induced apoptosis. In the inhibitory assay, the cytotoxic effect of 6-gingerol could be prevented by catalase. We suggest that 6-gingerol induced cell death by mediating reactive oxygen species such as hydrogen peroxide and the superoxide union. Therefore, the results showed that 6-gingerol induced apoptosis in HL-60 cells, not due to its antioxidative activity.

Original languageEnglish
Pages (from-to)641-645
Number of pages5
JournalIn Vivo
Volume17
Issue number6
Publication statusPublished - Nov 2003

Fingerprint

Ginger
HL-60 Cells
Antioxidants
Apoptosis
Cell death
Assays
Cell Death
Oxidative stress
DNA Fragmentation
Phenol
gingerol
Superoxides
Catalase
Hydrogen Peroxide
Reactive Oxygen Species
Leukemia
Oxidative Stress
DNA

Keywords

  • 6-Gingerol
  • Antioxidant
  • Apoptosis
  • Ginger
  • Zingiber officinale
  • Zingiberaceae

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effects of 6-Gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells. / Wang, Ching Chiung; Chen, Lih Geeng; Lee, Lain Tze; Yang, Ling-Ling.

In: In Vivo, Vol. 17, No. 6, 11.2003, p. 641-645.

Research output: Contribution to journalArticle

Wang, Ching Chiung ; Chen, Lih Geeng ; Lee, Lain Tze ; Yang, Ling-Ling. / Effects of 6-Gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells. In: In Vivo. 2003 ; Vol. 17, No. 6. pp. 641-645.
@article{66894d7c6b344465a486e73313384d5e,
title = "Effects of 6-Gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells",
abstract = "6-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger. In this paper, the antioxidative effects of 6-gingerol were detected by DPPH and DCFH assays and, as predicted, 6-gingerol as an antioxidant was shown to protect HL-60 cells from oxidative stress. Moreover, it induced cell death in promyelocytic leukemia HL-60 cells, caused DNA fragmentation and inhibited Bcl-2 expression in HL-60 cells. These results suggested that the inhibition of Bcl-2 expression in HL-60 cells might account for the mechanism of 6-gingerol-induced apoptosis. In the inhibitory assay, the cytotoxic effect of 6-gingerol could be prevented by catalase. We suggest that 6-gingerol induced cell death by mediating reactive oxygen species such as hydrogen peroxide and the superoxide union. Therefore, the results showed that 6-gingerol induced apoptosis in HL-60 cells, not due to its antioxidative activity.",
keywords = "6-Gingerol, Antioxidant, Apoptosis, Ginger, Zingiber officinale, Zingiberaceae",
author = "Wang, {Ching Chiung} and Chen, {Lih Geeng} and Lee, {Lain Tze} and Ling-Ling Yang",
year = "2003",
month = "11",
language = "English",
volume = "17",
pages = "641--645",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "6",

}

TY - JOUR

T1 - Effects of 6-Gingerol, an antioxidant from ginger, on inducing apoptosis in human leukemic HL-60 cells

AU - Wang, Ching Chiung

AU - Chen, Lih Geeng

AU - Lee, Lain Tze

AU - Yang, Ling-Ling

PY - 2003/11

Y1 - 2003/11

N2 - 6-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger. In this paper, the antioxidative effects of 6-gingerol were detected by DPPH and DCFH assays and, as predicted, 6-gingerol as an antioxidant was shown to protect HL-60 cells from oxidative stress. Moreover, it induced cell death in promyelocytic leukemia HL-60 cells, caused DNA fragmentation and inhibited Bcl-2 expression in HL-60 cells. These results suggested that the inhibition of Bcl-2 expression in HL-60 cells might account for the mechanism of 6-gingerol-induced apoptosis. In the inhibitory assay, the cytotoxic effect of 6-gingerol could be prevented by catalase. We suggest that 6-gingerol induced cell death by mediating reactive oxygen species such as hydrogen peroxide and the superoxide union. Therefore, the results showed that 6-gingerol induced apoptosis in HL-60 cells, not due to its antioxidative activity.

AB - 6-Gingerol, a naturally occurring plant phenol, is one of the major components of fresh ginger. In this paper, the antioxidative effects of 6-gingerol were detected by DPPH and DCFH assays and, as predicted, 6-gingerol as an antioxidant was shown to protect HL-60 cells from oxidative stress. Moreover, it induced cell death in promyelocytic leukemia HL-60 cells, caused DNA fragmentation and inhibited Bcl-2 expression in HL-60 cells. These results suggested that the inhibition of Bcl-2 expression in HL-60 cells might account for the mechanism of 6-gingerol-induced apoptosis. In the inhibitory assay, the cytotoxic effect of 6-gingerol could be prevented by catalase. We suggest that 6-gingerol induced cell death by mediating reactive oxygen species such as hydrogen peroxide and the superoxide union. Therefore, the results showed that 6-gingerol induced apoptosis in HL-60 cells, not due to its antioxidative activity.

KW - 6-Gingerol

KW - Antioxidant

KW - Apoptosis

KW - Ginger

KW - Zingiber officinale

KW - Zingiberaceae

UR - http://www.scopus.com/inward/record.url?scp=0842329588&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842329588&partnerID=8YFLogxK

M3 - Article

C2 - 14758732

AN - SCOPUS:0842329588

VL - 17

SP - 641

EP - 645

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 6

ER -