Effectiveness of synovial fluid mesenchymal stem cells embedded in alginate beads for treatment of steroid-induced avascular necrosis of the femoral head

Yu Pin Chen, Wei Chuan Chen, Kun Chuan Wang, Chih Hwa Chen

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: Treatment of early stage avascular necrosis of the femoral head (AVNFH) is still challenging for clinicians today. Core decompression with the implantation of mesenchymal stem cells (MSCs) has become popular and been proven to be an effective therapy for ANNFH. Synovial fluid MSCs, which can be easily harvested by joint aspiration, reportedly have the potential to differentiate into bone and cartilage. The purpose of this investigation is to evaluate the effectiveness of core decompression plus the implantation of alginate beads embedded with synovial fluid MSCs (ABSMSCs) in bone regeneration in the treatment of steroid-induced AVNFH in a rabbit model. Methods: An in vitro study is carried out to evaluate the bioactivity and osteogenic differentiation of synovial fluid MSCs in the environment formed by alginate beads. In an in vivo study, the application of ABSMSCs was combined with bone decompression to treat steroid-induced AVNFH in a rabbit model. Bone mineral density, radiography and histology were used to evaluate the bone growth of the femoral head after the rabbits had been euthanized 6 weeks after surgery. Results: The results obtained in vitro showed that the synovial fluid MSCs in the environment of alginate beads had the potential to differentiate toward bone growth. In vivo, the treatment of steroid-induced AVNFH in a rabbit model by core decompression plus the implantation of ABSMSCs preserved the bone density and sphericity of the femoral head and promoted bone regeneration. Conclusion: Implantation of ABSMSCs is a novel and effective therapy for AVNFH. Hopefully, this application will improve the outcome of early stage AVNFH and facilitate the harvesting of stem cells.

Original languageEnglish
Pages (from-to)657-666
Number of pages10
JournalJournal of Orthopaedic Science
Volume19
Issue number4
DOIs
Publication statusPublished - 2014

Fingerprint

Femur Head Necrosis
Synovial Fluid
Mesenchymal Stromal Cells
Steroids
Decompression
Rabbits
Bone Regeneration
Bone Development
Thigh
Bone Density
Therapeutics
Bone and Bones
alginic acid
Radiography
Cartilage
Histology
Stem Cells
Joints

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

Cite this

Effectiveness of synovial fluid mesenchymal stem cells embedded in alginate beads for treatment of steroid-induced avascular necrosis of the femoral head. / Chen, Yu Pin; Chen, Wei Chuan; Wang, Kun Chuan; Chen, Chih Hwa.

In: Journal of Orthopaedic Science, Vol. 19, No. 4, 2014, p. 657-666.

Research output: Contribution to journalArticle

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