Effect of water extracts from edible myrtaceae plants on uptake of 2-(n -(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose in TNF-α-treated FL83B mouse hepatocytes

Wen Chang Chang, Szu Chuan Shen

Research output: Contribution to journalArticle

16 Citations (Scopus)


This study investigated the glucose uptake activity of the water extracts from the leaves and fruit of edible Myrtaceae plants, including guava (Psidium guajava Linn.), wax apples [Syzygium samarangense (Blume) Merr. and L.M. Perry], Pu-Tau [Syzygium jambo (L.) Alston], and Kan-Shi Pu-Tau (Syzygium cumini Linn.) in FL83B mouse hepatocytes. The fluorescent dye 2-(n-(7-nitrobenz-2-oxa-1,3- diazol-4-yl)amino)-2-deoxyglucose was used to estimate the uptake ability of the cells. Glucose uptake test showed that pink wax apple fruit extract (PWFE) exhibits the highest glucose uptake activity, at an increment of 21% in the insulin-resistant FL83B mouse hepatocytes as compared with the TNF-α-treated control group. Vescalagin was isolated using column chromatography of PWFE. This compound, at the concentration of 6.25 μg/mL, exhibits the same glucose uptake improvement in insulin-resistant cells as PWFE at a 100-μg/mL dose. We postulate that vescalagin is an active component in PWFE that may alleviate the insulin resistance in mouse hepatocytes.

Original languageEnglish
Pages (from-to)236-243
Number of pages8
JournalPhytotherapy Research
Issue number2
Publication statusPublished - Feb 1 2013
Externally publishedYes



  • glucose uptake
  • insulin resistance
  • Myrtaceae plant
  • vescalagin
  • wax apple

ASJC Scopus subject areas

  • Pharmacology

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