Effect of tetramethylpyrazine on potassium channels to lower calcium concentration in cultured aortic smooth muscle cells

Kar Lok Wong, Paul Chan, Wei Chan Huang, Tzyy Lin Yang, I. Min Liu, Tung-Iuan Lai, Chin Chuan Tsai, Juei Tang Cheng

Research output: Contribution to journalArticle

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Abstract

1. Tetramethylpyrazine (TMP) is one of the active principles contained in Ligusticum chuanxiong Hort. (Umbelliferae), a herb that has been used widely in China to treat vascular disorders. 2. In an attempt to elucidate the possible mechanisms of action of TMP, the effect of TMP on intracellular calcium concentrations ([Ca2+]i) was investigated in cultured vascular smooth muscle (A7r5) cells using the Ca2+-sensitive dye Fura-2 as an indicator. 3. The increase in [Ca2+]i in A7r5 cells produced by vasopressin (1 μmol/L) or phenylephrine (1 μmol/L) was attenuated by TMP in a concentration-dependent manner. Only inhibitors specific to ATP-sensitive potassium (KATP) channels or small conductance calcium-activated potassium (SKCa) channels attenuated the action of TMP (10·μmol/L) on [Ca2+]i. However, blockers of other K+ channels failed to modify the inhibitory action of TMP (10 μmol/L) on [Ca2+]i. 4. The action of TMP on membrane potential in A7r5 cells was monitored by the fluorescence of bisoxonol. Tetramethylpyrazine caused a concentration-dependent inhibition of changes in membrane potential elicited by KCl (20 mmol/L) or phenylephrine (1 μmol/L), an effect that was totally reversed by glibenclamide (100 μmol/L) and apamin (100 nmol/L) in combination. 5. The results obtained indicate that the decrease in [Ca2+]i in A7r5 cells produced by TMP is mediated mainly by opening of KATP and/or SKCa channels.

Original languageEnglish
Pages (from-to)793-798
Number of pages6
JournalClinical and Experimental Pharmacology and Physiology
Volume30
Issue number10
DOIs
Publication statusPublished - Oct 2003

Fingerprint

Potassium Channels
Smooth Muscle Myocytes
Calcium
KATP Channels
Phenylephrine
Membrane Potentials
Small-Conductance Calcium-Activated Potassium Channels
Ligusticum
Apiaceae
Apamin
tetramethylpyrazine
Glyburide
Fura-2
Vasopressins
Vascular Smooth Muscle
Blood Vessels
China
Fluorescence

Keywords

  • Bisoxonol
  • Cultured vascular smooth muscle cells
  • Fura-2
  • Intracellular calcium concentrations
  • Potassium channel blockers
  • Tetramethylpyrazine

ASJC Scopus subject areas

  • Physiology
  • Pharmacology (medical)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Effect of tetramethylpyrazine on potassium channels to lower calcium concentration in cultured aortic smooth muscle cells. / Wong, Kar Lok; Chan, Paul; Huang, Wei Chan; Yang, Tzyy Lin; Liu, I. Min; Lai, Tung-Iuan; Tsai, Chin Chuan; Cheng, Juei Tang.

In: Clinical and Experimental Pharmacology and Physiology, Vol. 30, No. 10, 10.2003, p. 793-798.

Research output: Contribution to journalArticle

Wong, Kar Lok ; Chan, Paul ; Huang, Wei Chan ; Yang, Tzyy Lin ; Liu, I. Min ; Lai, Tung-Iuan ; Tsai, Chin Chuan ; Cheng, Juei Tang. / Effect of tetramethylpyrazine on potassium channels to lower calcium concentration in cultured aortic smooth muscle cells. In: Clinical and Experimental Pharmacology and Physiology. 2003 ; Vol. 30, No. 10. pp. 793-798.
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AU - Wong, Kar Lok

AU - Chan, Paul

AU - Huang, Wei Chan

AU - Yang, Tzyy Lin

AU - Liu, I. Min

AU - Lai, Tung-Iuan

AU - Tsai, Chin Chuan

AU - Cheng, Juei Tang

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AB - 1. Tetramethylpyrazine (TMP) is one of the active principles contained in Ligusticum chuanxiong Hort. (Umbelliferae), a herb that has been used widely in China to treat vascular disorders. 2. In an attempt to elucidate the possible mechanisms of action of TMP, the effect of TMP on intracellular calcium concentrations ([Ca2+]i) was investigated in cultured vascular smooth muscle (A7r5) cells using the Ca2+-sensitive dye Fura-2 as an indicator. 3. The increase in [Ca2+]i in A7r5 cells produced by vasopressin (1 μmol/L) or phenylephrine (1 μmol/L) was attenuated by TMP in a concentration-dependent manner. Only inhibitors specific to ATP-sensitive potassium (KATP) channels or small conductance calcium-activated potassium (SKCa) channels attenuated the action of TMP (10·μmol/L) on [Ca2+]i. However, blockers of other K+ channels failed to modify the inhibitory action of TMP (10 μmol/L) on [Ca2+]i. 4. The action of TMP on membrane potential in A7r5 cells was monitored by the fluorescence of bisoxonol. Tetramethylpyrazine caused a concentration-dependent inhibition of changes in membrane potential elicited by KCl (20 mmol/L) or phenylephrine (1 μmol/L), an effect that was totally reversed by glibenclamide (100 μmol/L) and apamin (100 nmol/L) in combination. 5. The results obtained indicate that the decrease in [Ca2+]i in A7r5 cells produced by TMP is mediated mainly by opening of KATP and/or SKCa channels.

KW - Bisoxonol

KW - Cultured vascular smooth muscle cells

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KW - Intracellular calcium concentrations

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