Effect of tetramethylpyrazine on blood pressure in hypertensive rats and calcium mobilization in vascular smooth muscle cells

M. F. Tsai, T. H. Chen, J. C. Liu, Y. J. Chen, M. H. Hsieh, P. Chan

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background: Tetramethylpyrazine (TMP) is a plant alkaloid isolated from the traditional Chinese herb Chuang Xiong. It has been proved to have antihypertensive effect on anestheized animals. This study was undertaken to evaluate its effect on ambulatory hypertensive rats and its mechanism of antihypertension. Materials and Methods: Deoxycorticosterone acetate (DOCA)- induced hypertensive rats comprising 4 groups (each n = 8) were used for study. TMP dissolved in normal saline was given intraperitoneally at different dosages (1,5,10 mg/kg) and blood pressure was recorded by a noninvasive tail-cuff monitor. Aortic smooth muscle cells (A7r5) were employed to evaluate calcium influx inhibition by using Fura-2 calcium-sensitive dye. Results: The hypotensive effect of TMP on DOCA-salt sensitive hypertensive rats was dose-dependent; the maximal blood pressure decrease was 28.2 ± 5.0 mm Hg at the dosage of 10 mg/kg. By using calcium influx stimulating agents phenylephrine (an α adrenergic agonist) and arginine vasopressin to induce calcium influx, TMP showed a dose-dependent calcium influx inhibition. Conclusion: TMP isolated from traditional herb has an effective antihypertensive action and this antihypertension is probably due to intracellular calcium influx inhibition.

Original languageEnglish
Pages (from-to)3-8
Number of pages6
JournalActa Cardiologica Sinica
Volume17
Issue number1
Publication statusPublished - 2001

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Vascular Smooth Muscle
Smooth Muscle Myocytes
Blood Pressure
Calcium
Desoxycorticosterone
Antihypertensive Agents
Acetates
Adrenergic Agonists
Arginine Vasopressin
Fura-2
Phenylephrine
Alkaloids
tetramethylpyrazine
Tail
Coloring Agents
Salts

Keywords

  • Calcium influx
  • DOCA salt
  • Sensitive hypertensive rats
  • Tetramethylpyrazine

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

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title = "Effect of tetramethylpyrazine on blood pressure in hypertensive rats and calcium mobilization in vascular smooth muscle cells",
abstract = "Background: Tetramethylpyrazine (TMP) is a plant alkaloid isolated from the traditional Chinese herb Chuang Xiong. It has been proved to have antihypertensive effect on anestheized animals. This study was undertaken to evaluate its effect on ambulatory hypertensive rats and its mechanism of antihypertension. Materials and Methods: Deoxycorticosterone acetate (DOCA)- induced hypertensive rats comprising 4 groups (each n = 8) were used for study. TMP dissolved in normal saline was given intraperitoneally at different dosages (1,5,10 mg/kg) and blood pressure was recorded by a noninvasive tail-cuff monitor. Aortic smooth muscle cells (A7r5) were employed to evaluate calcium influx inhibition by using Fura-2 calcium-sensitive dye. Results: The hypotensive effect of TMP on DOCA-salt sensitive hypertensive rats was dose-dependent; the maximal blood pressure decrease was 28.2 ± 5.0 mm Hg at the dosage of 10 mg/kg. By using calcium influx stimulating agents phenylephrine (an α adrenergic agonist) and arginine vasopressin to induce calcium influx, TMP showed a dose-dependent calcium influx inhibition. Conclusion: TMP isolated from traditional herb has an effective antihypertensive action and this antihypertension is probably due to intracellular calcium influx inhibition.",
keywords = "Calcium influx, DOCA salt, Sensitive hypertensive rats, Tetramethylpyrazine",
author = "Tsai, {M. F.} and Chen, {T. H.} and Liu, {J. C.} and Chen, {Y. J.} and Hsieh, {M. H.} and P. Chan",
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TY - JOUR

T1 - Effect of tetramethylpyrazine on blood pressure in hypertensive rats and calcium mobilization in vascular smooth muscle cells

AU - Tsai, M. F.

AU - Chen, T. H.

AU - Liu, J. C.

AU - Chen, Y. J.

AU - Hsieh, M. H.

AU - Chan, P.

PY - 2001

Y1 - 2001

N2 - Background: Tetramethylpyrazine (TMP) is a plant alkaloid isolated from the traditional Chinese herb Chuang Xiong. It has been proved to have antihypertensive effect on anestheized animals. This study was undertaken to evaluate its effect on ambulatory hypertensive rats and its mechanism of antihypertension. Materials and Methods: Deoxycorticosterone acetate (DOCA)- induced hypertensive rats comprising 4 groups (each n = 8) were used for study. TMP dissolved in normal saline was given intraperitoneally at different dosages (1,5,10 mg/kg) and blood pressure was recorded by a noninvasive tail-cuff monitor. Aortic smooth muscle cells (A7r5) were employed to evaluate calcium influx inhibition by using Fura-2 calcium-sensitive dye. Results: The hypotensive effect of TMP on DOCA-salt sensitive hypertensive rats was dose-dependent; the maximal blood pressure decrease was 28.2 ± 5.0 mm Hg at the dosage of 10 mg/kg. By using calcium influx stimulating agents phenylephrine (an α adrenergic agonist) and arginine vasopressin to induce calcium influx, TMP showed a dose-dependent calcium influx inhibition. Conclusion: TMP isolated from traditional herb has an effective antihypertensive action and this antihypertension is probably due to intracellular calcium influx inhibition.

AB - Background: Tetramethylpyrazine (TMP) is a plant alkaloid isolated from the traditional Chinese herb Chuang Xiong. It has been proved to have antihypertensive effect on anestheized animals. This study was undertaken to evaluate its effect on ambulatory hypertensive rats and its mechanism of antihypertension. Materials and Methods: Deoxycorticosterone acetate (DOCA)- induced hypertensive rats comprising 4 groups (each n = 8) were used for study. TMP dissolved in normal saline was given intraperitoneally at different dosages (1,5,10 mg/kg) and blood pressure was recorded by a noninvasive tail-cuff monitor. Aortic smooth muscle cells (A7r5) were employed to evaluate calcium influx inhibition by using Fura-2 calcium-sensitive dye. Results: The hypotensive effect of TMP on DOCA-salt sensitive hypertensive rats was dose-dependent; the maximal blood pressure decrease was 28.2 ± 5.0 mm Hg at the dosage of 10 mg/kg. By using calcium influx stimulating agents phenylephrine (an α adrenergic agonist) and arginine vasopressin to induce calcium influx, TMP showed a dose-dependent calcium influx inhibition. Conclusion: TMP isolated from traditional herb has an effective antihypertensive action and this antihypertension is probably due to intracellular calcium influx inhibition.

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KW - DOCA salt

KW - Sensitive hypertensive rats

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