Abstract
BACKGROUND: Surfactant is a useful vehicle for the intratracheal delivery of medicine to the distal lung. The aim of this study was to analyze the effect of intratracheal surfactant and budesonide instillation on the pulmonary distribution of fluorescent dye in mice.
METHODS: Male athymic nude mice were assigned randomly as controls, fluorescent dye, fluorescent dye + surfactant (50 mg/kg), fluorescent dye + budesonide (0.25 mg/kg), and fluorescent dye + surfactant + budesonide groups. A total volume of 60 μL fluorescent solutions was intratracheally injected and followed by 60 μL of air. We photographed and measured fluorescence in the lungs, from the back, 15 minutes after intratracheal administration using an IVIS Xenogen imaging instrument.
RESULTS: The fluorescent dye (1,1'-dioctadecyltetramethyl indotricarbocyanine iodide) was most strongly detected near the trachea and weakly detected in the lungs in mice administered with fluorescent solutions. Almost no fluorescence was seen in the lung region of control mice. Intratracheal administration of surfactant or budesonide increased fluorescent intensity compared with control mice. Combined administration of surfactant and budesonide further increased fluorescent intensity compared with mice given surfactant or budesonide alone.
CONCLUSION: Surfactant and budesonide enhance the pulmonary distribution of fluorescent dye in mice.
| Original language | English |
|---|---|
| Pages (from-to) | 19-24 |
| Number of pages | 6 |
| Journal | Pediatrics and Neonatology |
| Volume | 56 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 1 2015 |
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Keywords
- budesonide
- fluorescence
- in vivo imaging system
- surfactant
ASJC Scopus subject areas
- Pediatrics, Perinatology, and Child Health
Cite this
Effect of surfactant and budesonide on the pulmonary distribution of fluorescent dye in mice. / Huang, Liang Ti; Yeh, Tsu Fu; Kuo, Yu Lin; Chen, Pin Chuan; Chen, Chung Ming.
In: Pediatrics and Neonatology, Vol. 56, No. 1, 01.02.2015, p. 19-24.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of surfactant and budesonide on the pulmonary distribution of fluorescent dye in mice
AU - Huang, Liang Ti
AU - Yeh, Tsu Fu
AU - Kuo, Yu Lin
AU - Chen, Pin Chuan
AU - Chen, Chung Ming
PY - 2015/2/1
Y1 - 2015/2/1
N2 - BACKGROUND: Surfactant is a useful vehicle for the intratracheal delivery of medicine to the distal lung. The aim of this study was to analyze the effect of intratracheal surfactant and budesonide instillation on the pulmonary distribution of fluorescent dye in mice.METHODS: Male athymic nude mice were assigned randomly as controls, fluorescent dye, fluorescent dye + surfactant (50 mg/kg), fluorescent dye + budesonide (0.25 mg/kg), and fluorescent dye + surfactant + budesonide groups. A total volume of 60 μL fluorescent solutions was intratracheally injected and followed by 60 μL of air. We photographed and measured fluorescence in the lungs, from the back, 15 minutes after intratracheal administration using an IVIS Xenogen imaging instrument.RESULTS: The fluorescent dye (1,1'-dioctadecyltetramethyl indotricarbocyanine iodide) was most strongly detected near the trachea and weakly detected in the lungs in mice administered with fluorescent solutions. Almost no fluorescence was seen in the lung region of control mice. Intratracheal administration of surfactant or budesonide increased fluorescent intensity compared with control mice. Combined administration of surfactant and budesonide further increased fluorescent intensity compared with mice given surfactant or budesonide alone.CONCLUSION: Surfactant and budesonide enhance the pulmonary distribution of fluorescent dye in mice.
AB - BACKGROUND: Surfactant is a useful vehicle for the intratracheal delivery of medicine to the distal lung. The aim of this study was to analyze the effect of intratracheal surfactant and budesonide instillation on the pulmonary distribution of fluorescent dye in mice.METHODS: Male athymic nude mice were assigned randomly as controls, fluorescent dye, fluorescent dye + surfactant (50 mg/kg), fluorescent dye + budesonide (0.25 mg/kg), and fluorescent dye + surfactant + budesonide groups. A total volume of 60 μL fluorescent solutions was intratracheally injected and followed by 60 μL of air. We photographed and measured fluorescence in the lungs, from the back, 15 minutes after intratracheal administration using an IVIS Xenogen imaging instrument.RESULTS: The fluorescent dye (1,1'-dioctadecyltetramethyl indotricarbocyanine iodide) was most strongly detected near the trachea and weakly detected in the lungs in mice administered with fluorescent solutions. Almost no fluorescence was seen in the lung region of control mice. Intratracheal administration of surfactant or budesonide increased fluorescent intensity compared with control mice. Combined administration of surfactant and budesonide further increased fluorescent intensity compared with mice given surfactant or budesonide alone.CONCLUSION: Surfactant and budesonide enhance the pulmonary distribution of fluorescent dye in mice.
KW - budesonide
KW - fluorescence
KW - in vivo imaging system
KW - surfactant
UR - http://www.scopus.com/inward/record.url?scp=85028238120&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85028238120&partnerID=8YFLogxK
U2 - 10.1016/j.pedneo.2014.04.009
DO - 10.1016/j.pedneo.2014.04.009
M3 - Article
C2 - 25199848
AN - SCOPUS:85028238120
VL - 56
SP - 19
EP - 24
JO - Pediatrics and Neonatology
JF - Pediatrics and Neonatology
SN - 1875-9572
IS - 1
ER -