Effect of substance P and protein kinase inhibitors on β-amyloid peptide-induced proliferation of cultured brain cells

Vijendra K. Singh, Jui Fen Cheng, Sy Jye C Leu

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The present study investigated the effect of substance P (SP) and protein kinase inhibitors (H7 and HA1004) on β-amyloid peptide-induced proliferation of neonatal rat brain cells in primary cultures. The β-amyloid peptide1-28 (designated as βAP28), at nanomolar concentrations (10-9 M), significantly (P {precedes above single-line equals sign} 0.05) increased the proliferation of brain cells (presumably non-neuronal) as measured by [3H]thymidine uptake into DNA (mitogenesis). The effect was dependent on time of cultured, concentration of βAP28, and presence of fetal calf serum. The supplementation of SP into cell cultures at time zero reversed the proliferative response of βAP28. Moreover, the βAP28-induced proliferation was inhibited by protein kinase inhibitor H7, but not by HA1004. Since H7 is a selective protein kinase C (PKC) inhibitor and SP action involves PKC, we conclude that βAP28 induces normal brain cell proliferation through PKC pathway of cell signaling.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalBrain Research
Volume660
Issue number2
DOIs
Publication statusPublished - Oct 17 1994
Externally publishedYes

Keywords

  • Alzheimer's disease
  • Brain cell proliferation
  • Growth factor
  • Signal transduction
  • β-Amyloid protein

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

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