Effect of smoking habit on the frequency of micronuclei in human lymphocytes

Results from the Human MicroNucleus project

Stefano Bonassi, Monica Neri, Cecilia Lando, Marcello Ceppi, Yi Ping Lin, Wushou P. Chang, Nina Holland, Micheline Kirsch-Volders, Errol Zeiger, Michael Fenech, Sadayuki Ban, Roberto Barale, Maria Paola Bigatti, Claudia Bolognesi, Cao Jia, Marina Di Giorgio, Lynnette R. Ferguson, Aleksandra Fucic, Patrizia Hrelia, Ayyathan P. Krishnaja & 12 others Tung Kwang Lee, Lucia Migliore, Ludmilla Mikhalevich, Ekaterina Mirkova, Pasquale Mosesso, Wolfgang Ulrich Müller, Youichi Odagiri, Maria Rosaria Scarfi, Elena Szabova, Irena Vorobtsova, Anne Vral, Andrea Zijno

Research output: Contribution to journalArticle

253 Citations (Scopus)

Abstract

The effect of tobacco smoking on the frequency of micronuclei (MN) in human lymphocytes has been the object of many population studies. In most reports, the results were unexpectedly negative, and in many instances smokers had lower frequencies of MN than non-smokers. A pooled re-analysis of 24 databases from the HUMN international collaborative project has been performed with the aim of understanding the impact of smoking habits on MN frequency. The complete database included 5710 subjects, with 3501 non-smokers, 1409 current smokers, and 800 former smokers, among subjects in occupational and environmental surveys. The overall result of the re-analysis confirmed the small decrease of MN frequencies in current smokers (frequency ratio (FR)=0.97, 95% confidence interval (CI)=0.93-1.01) and in former smokers (FR=0.96, 95% CI=0.91-1.01), when compared to non-smokers. MN frequency was not influenced by the number of cigarettes smoked per day among subjects occupationally exposed to genotoxic agents, whereas a typical U-shaped curve is observed for non-exposed smokers, showing a significant increase of MN frequency in individuals smoking 30 cigarettes or more per day (FR=1.59, 95% CI=1.35-1.88). This analysis confirmed that smokers do not experience an overall increase in MN frequency, although when the interaction with occupational exposure is taken into account, heavy smokers were the only group showing a significant increase in genotoxic damage as measured by the micronucleus assay in lymphocytes. From these results some general recommendations for the design of biomonitoring studies involving smokers can be formulated. Quantitative data about smoking habit should always be collected because, in the absence of such data, the simple comparison of smokers versus non-smokers could be misleading. The sub-group of heavy smokers (≥30 cigarettes per day) should be specifically evaluated whenever it is large enough to satisfy statistical requirements. The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested.

Original languageEnglish
Pages (from-to)155-166
Number of pages12
JournalMutation Research - Reviews in Mutation Research
Volume543
Issue number2
DOIs
Publication statusPublished - Mar 2003
Externally publishedYes

Fingerprint

Habits
Smoking
Lymphocytes
Confidence Intervals
Occupational Exposure
Tobacco Products
Databases
Micronucleus Tests
Environmental Monitoring
Population

Keywords

  • Human lymphocytes
  • Micronuclei
  • Pooled analysis
  • Smoking habit

ASJC Scopus subject areas

  • Health, Toxicology and Mutagenesis

Cite this

Effect of smoking habit on the frequency of micronuclei in human lymphocytes : Results from the Human MicroNucleus project. / Bonassi, Stefano; Neri, Monica; Lando, Cecilia; Ceppi, Marcello; Lin, Yi Ping; Chang, Wushou P.; Holland, Nina; Kirsch-Volders, Micheline; Zeiger, Errol; Fenech, Michael; Ban, Sadayuki; Barale, Roberto; Bigatti, Maria Paola; Bolognesi, Claudia; Jia, Cao; Di Giorgio, Marina; Ferguson, Lynnette R.; Fucic, Aleksandra; Hrelia, Patrizia; Krishnaja, Ayyathan P.; Lee, Tung Kwang; Migliore, Lucia; Mikhalevich, Ludmilla; Mirkova, Ekaterina; Mosesso, Pasquale; Müller, Wolfgang Ulrich; Odagiri, Youichi; Scarfi, Maria Rosaria; Szabova, Elena; Vorobtsova, Irena; Vral, Anne; Zijno, Andrea.

In: Mutation Research - Reviews in Mutation Research, Vol. 543, No. 2, 03.2003, p. 155-166.

Research output: Contribution to journalArticle

Bonassi, S, Neri, M, Lando, C, Ceppi, M, Lin, YP, Chang, WP, Holland, N, Kirsch-Volders, M, Zeiger, E, Fenech, M, Ban, S, Barale, R, Bigatti, MP, Bolognesi, C, Jia, C, Di Giorgio, M, Ferguson, LR, Fucic, A, Hrelia, P, Krishnaja, AP, Lee, TK, Migliore, L, Mikhalevich, L, Mirkova, E, Mosesso, P, Müller, WU, Odagiri, Y, Scarfi, MR, Szabova, E, Vorobtsova, I, Vral, A & Zijno, A 2003, 'Effect of smoking habit on the frequency of micronuclei in human lymphocytes: Results from the Human MicroNucleus project', Mutation Research - Reviews in Mutation Research, vol. 543, no. 2, pp. 155-166. https://doi.org/10.1016/S1383-5742(03)00013-9
Bonassi, Stefano ; Neri, Monica ; Lando, Cecilia ; Ceppi, Marcello ; Lin, Yi Ping ; Chang, Wushou P. ; Holland, Nina ; Kirsch-Volders, Micheline ; Zeiger, Errol ; Fenech, Michael ; Ban, Sadayuki ; Barale, Roberto ; Bigatti, Maria Paola ; Bolognesi, Claudia ; Jia, Cao ; Di Giorgio, Marina ; Ferguson, Lynnette R. ; Fucic, Aleksandra ; Hrelia, Patrizia ; Krishnaja, Ayyathan P. ; Lee, Tung Kwang ; Migliore, Lucia ; Mikhalevich, Ludmilla ; Mirkova, Ekaterina ; Mosesso, Pasquale ; Müller, Wolfgang Ulrich ; Odagiri, Youichi ; Scarfi, Maria Rosaria ; Szabova, Elena ; Vorobtsova, Irena ; Vral, Anne ; Zijno, Andrea. / Effect of smoking habit on the frequency of micronuclei in human lymphocytes : Results from the Human MicroNucleus project. In: Mutation Research - Reviews in Mutation Research. 2003 ; Vol. 543, No. 2. pp. 155-166.
@article{8ecbc944c2d342c09e85afb96ed2cb58,
title = "Effect of smoking habit on the frequency of micronuclei in human lymphocytes: Results from the Human MicroNucleus project",
abstract = "The effect of tobacco smoking on the frequency of micronuclei (MN) in human lymphocytes has been the object of many population studies. In most reports, the results were unexpectedly negative, and in many instances smokers had lower frequencies of MN than non-smokers. A pooled re-analysis of 24 databases from the HUMN international collaborative project has been performed with the aim of understanding the impact of smoking habits on MN frequency. The complete database included 5710 subjects, with 3501 non-smokers, 1409 current smokers, and 800 former smokers, among subjects in occupational and environmental surveys. The overall result of the re-analysis confirmed the small decrease of MN frequencies in current smokers (frequency ratio (FR)=0.97, 95{\%} confidence interval (CI)=0.93-1.01) and in former smokers (FR=0.96, 95{\%} CI=0.91-1.01), when compared to non-smokers. MN frequency was not influenced by the number of cigarettes smoked per day among subjects occupationally exposed to genotoxic agents, whereas a typical U-shaped curve is observed for non-exposed smokers, showing a significant increase of MN frequency in individuals smoking 30 cigarettes or more per day (FR=1.59, 95{\%} CI=1.35-1.88). This analysis confirmed that smokers do not experience an overall increase in MN frequency, although when the interaction with occupational exposure is taken into account, heavy smokers were the only group showing a significant increase in genotoxic damage as measured by the micronucleus assay in lymphocytes. From these results some general recommendations for the design of biomonitoring studies involving smokers can be formulated. Quantitative data about smoking habit should always be collected because, in the absence of such data, the simple comparison of smokers versus non-smokers could be misleading. The sub-group of heavy smokers (≥30 cigarettes per day) should be specifically evaluated whenever it is large enough to satisfy statistical requirements. The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested.",
keywords = "Human lymphocytes, Micronuclei, Pooled analysis, Smoking habit",
author = "Stefano Bonassi and Monica Neri and Cecilia Lando and Marcello Ceppi and Lin, {Yi Ping} and Chang, {Wushou P.} and Nina Holland and Micheline Kirsch-Volders and Errol Zeiger and Michael Fenech and Sadayuki Ban and Roberto Barale and Bigatti, {Maria Paola} and Claudia Bolognesi and Cao Jia and {Di Giorgio}, Marina and Ferguson, {Lynnette R.} and Aleksandra Fucic and Patrizia Hrelia and Krishnaja, {Ayyathan P.} and Lee, {Tung Kwang} and Lucia Migliore and Ludmilla Mikhalevich and Ekaterina Mirkova and Pasquale Mosesso and M{\"u}ller, {Wolfgang Ulrich} and Youichi Odagiri and Scarfi, {Maria Rosaria} and Elena Szabova and Irena Vorobtsova and Anne Vral and Andrea Zijno",
year = "2003",
month = "3",
doi = "10.1016/S1383-5742(03)00013-9",
language = "English",
volume = "543",
pages = "155--166",
journal = "Mutation Research - Reviews in Mutation Research",
issn = "1383-5742",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Effect of smoking habit on the frequency of micronuclei in human lymphocytes

T2 - Results from the Human MicroNucleus project

AU - Bonassi, Stefano

AU - Neri, Monica

AU - Lando, Cecilia

AU - Ceppi, Marcello

AU - Lin, Yi Ping

AU - Chang, Wushou P.

AU - Holland, Nina

AU - Kirsch-Volders, Micheline

AU - Zeiger, Errol

AU - Fenech, Michael

AU - Ban, Sadayuki

AU - Barale, Roberto

AU - Bigatti, Maria Paola

AU - Bolognesi, Claudia

AU - Jia, Cao

AU - Di Giorgio, Marina

AU - Ferguson, Lynnette R.

AU - Fucic, Aleksandra

AU - Hrelia, Patrizia

AU - Krishnaja, Ayyathan P.

AU - Lee, Tung Kwang

AU - Migliore, Lucia

AU - Mikhalevich, Ludmilla

AU - Mirkova, Ekaterina

AU - Mosesso, Pasquale

AU - Müller, Wolfgang Ulrich

AU - Odagiri, Youichi

AU - Scarfi, Maria Rosaria

AU - Szabova, Elena

AU - Vorobtsova, Irena

AU - Vral, Anne

AU - Zijno, Andrea

PY - 2003/3

Y1 - 2003/3

N2 - The effect of tobacco smoking on the frequency of micronuclei (MN) in human lymphocytes has been the object of many population studies. In most reports, the results were unexpectedly negative, and in many instances smokers had lower frequencies of MN than non-smokers. A pooled re-analysis of 24 databases from the HUMN international collaborative project has been performed with the aim of understanding the impact of smoking habits on MN frequency. The complete database included 5710 subjects, with 3501 non-smokers, 1409 current smokers, and 800 former smokers, among subjects in occupational and environmental surveys. The overall result of the re-analysis confirmed the small decrease of MN frequencies in current smokers (frequency ratio (FR)=0.97, 95% confidence interval (CI)=0.93-1.01) and in former smokers (FR=0.96, 95% CI=0.91-1.01), when compared to non-smokers. MN frequency was not influenced by the number of cigarettes smoked per day among subjects occupationally exposed to genotoxic agents, whereas a typical U-shaped curve is observed for non-exposed smokers, showing a significant increase of MN frequency in individuals smoking 30 cigarettes or more per day (FR=1.59, 95% CI=1.35-1.88). This analysis confirmed that smokers do not experience an overall increase in MN frequency, although when the interaction with occupational exposure is taken into account, heavy smokers were the only group showing a significant increase in genotoxic damage as measured by the micronucleus assay in lymphocytes. From these results some general recommendations for the design of biomonitoring studies involving smokers can be formulated. Quantitative data about smoking habit should always be collected because, in the absence of such data, the simple comparison of smokers versus non-smokers could be misleading. The sub-group of heavy smokers (≥30 cigarettes per day) should be specifically evaluated whenever it is large enough to satisfy statistical requirements. The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested.

AB - The effect of tobacco smoking on the frequency of micronuclei (MN) in human lymphocytes has been the object of many population studies. In most reports, the results were unexpectedly negative, and in many instances smokers had lower frequencies of MN than non-smokers. A pooled re-analysis of 24 databases from the HUMN international collaborative project has been performed with the aim of understanding the impact of smoking habits on MN frequency. The complete database included 5710 subjects, with 3501 non-smokers, 1409 current smokers, and 800 former smokers, among subjects in occupational and environmental surveys. The overall result of the re-analysis confirmed the small decrease of MN frequencies in current smokers (frequency ratio (FR)=0.97, 95% confidence interval (CI)=0.93-1.01) and in former smokers (FR=0.96, 95% CI=0.91-1.01), when compared to non-smokers. MN frequency was not influenced by the number of cigarettes smoked per day among subjects occupationally exposed to genotoxic agents, whereas a typical U-shaped curve is observed for non-exposed smokers, showing a significant increase of MN frequency in individuals smoking 30 cigarettes or more per day (FR=1.59, 95% CI=1.35-1.88). This analysis confirmed that smokers do not experience an overall increase in MN frequency, although when the interaction with occupational exposure is taken into account, heavy smokers were the only group showing a significant increase in genotoxic damage as measured by the micronucleus assay in lymphocytes. From these results some general recommendations for the design of biomonitoring studies involving smokers can be formulated. Quantitative data about smoking habit should always be collected because, in the absence of such data, the simple comparison of smokers versus non-smokers could be misleading. The sub-group of heavy smokers (≥30 cigarettes per day) should be specifically evaluated whenever it is large enough to satisfy statistical requirements. The presence of an interaction between smoking habit and occupational exposure to genotoxic agents should be always tested.

KW - Human lymphocytes

KW - Micronuclei

KW - Pooled analysis

KW - Smoking habit

UR - http://www.scopus.com/inward/record.url?scp=0037339166&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037339166&partnerID=8YFLogxK

U2 - 10.1016/S1383-5742(03)00013-9

DO - 10.1016/S1383-5742(03)00013-9

M3 - Article

VL - 543

SP - 155

EP - 166

JO - Mutation Research - Reviews in Mutation Research

JF - Mutation Research - Reviews in Mutation Research

SN - 1383-5742

IS - 2

ER -