Effect of SACCHACHITIN on keratinocyte proliferation and the expressions of type I collagen and tissue-transglutaminase during skin wound healing

Wei Sheng Hung, Wen-FuThomas Lai, Bowen Leu, Ching Hua Su, Chia Lang Fang, Yu-Hui Tsai

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6 Citations (Scopus)


SACCHACHITIN is a skin wound-healing membrane made of residual fruiting body of Ganoderma tsugae. Its effect on proliferating cell nuclear antigen (PCNA) expression in actively proliferating cells, type I collagen expression and tissue remodeling in the healing tissue, and the association of tissue-transglutaminase (t-TGase) with wound healing were investigated by immunohistochemical staining. The results demonstrated that PCNA expressed in keratinocytes since day 1 in the SACCHACHITIN group and persisted during entire healing process. In contrast, it was barely detectable on day 3 in the control group. At keratinocyte layer, the SACCHACHITIN group exhibited more type I collagen than did the control group since day 1. At scar tissue, type I collagen was positively stained in the SACCHACHITIN group since day 7 but not in the control group till day 12. Furthermore, t-TGase was strongly expressed on the inner wall of angiogenic vessels on day 5 of the control group but not on that of the SACCHACHITIN group until day 10. The earlier expressions of PCNA and type I collagen in the keratinocyte layer may lead to accelerated skin wound healing. In addition, the later expression of t-TGase, an indicator of apoptosis, on the inner wall of angiogenic capillaries in the SACCHACHITIN group may indicate a longer period of blood supply to the wound area, thus facilitating wound healing. These observed phenomena might underline the beneficial effects of SACCHACHITIN membrane on rapid wound healing.

Original languageEnglish
Pages (from-to)122-129
Number of pages8
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Issue number1
Publication statusPublished - Jul 15 2004



  • Ganoderma tsugae
  • Proliferating cell nuclear antigen (PCNA)
  • Skin wound healing
  • Tissue-transglutaminase (t-TGase)
  • Type I collagen

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

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