Effect of Reishi polysaccharides on human stem/progenitor cells

Wan Yu Chen, Wen Bin Yang, Chi Huey Wong, Tzu-Bi Shih

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The polysaccharide fraction of Ganoderma lucidum (F3) was found to benefit our health in many ways by influencing the activity of tissue stem/progenitor cells. In this study, F3 was found to promote the adipose tissue MSCs' aggregation and chondrosphere formation, with the increase of CAM (N-CAM, I-CAM) expressions and autokine (BMP-2, IL-11, and aggrecan) secretions, in an in vitro chondrogenesis assay. In a stem cell expansion culture, it possesses the thrombopoietin (TPO) and GM-CSF like functions to enhance the survival/renewal abilities of primitive hematopoietic stem/progenitor cells (HSCs). F3 was found to promote the dendrite growth of blood mononuclear cells (MNCs) and the expression of cell adhesion molecules in the formation of immature dendritic cells (DC). On the other hand, F3 exhibited inhibitory effects on blood endothelial progenitor (EPC) colony formation, with concomitant reduction of cell surface endoglin (CD105) and vascular endothelial growth factor receptor-3 (VEGFR-3) marker expressions, in the presence of angiogenic factors. A further cytokine array analysis revealed that F3 indeed inhibited the angiogenin synthesis and enhanced IL-1, MCP-1, MIP-1, RANTES, and GRO productions in the blood EPC derivation culture. Collectively, we have demonstrated that the polysaccharide fraction of G. lucidum F3 exhibits cytokine and chemokine like functions which are beneficial to human tissue stem/progenitor cells by modulating their CAM expressions and biological activities. These findings provide us a better the observation that F3 glycopolysaccharides indeed possesses anti-angiogenic and immune-modulating functions and promotes hematopoietic stem/progenitor cell homing for better human tissue protection, reducing disease progression and health.

Original languageEnglish
Pages (from-to)8583-8591
Number of pages9
JournalBioorganic and Medicinal Chemistry
Volume18
Issue number24
DOIs
Publication statusPublished - Dec 15 2010

Fingerprint

Reishi
Stem cells
Polysaccharides
Computer aided manufacturing
Hematopoietic Stem Cells
Stem Cells
Tissue
Blood
Vascular Endothelial Growth Factor Receptor-3
Health
Interleukin-11
Cytokines
Thrombopoietin
Aggrecans
Chondrogenesis
Chemokine CCL5
Angiogenesis Inducing Agents
Cell Adhesion Molecules
Insurance Benefits
Granulocyte-Macrophage Colony-Stimulating Factor

Keywords

  • Anti-angiogenesis
  • Cell adhesion molecules
  • Human tissue stem/progenitor cells
  • Immune defensive chemokines
  • Reishi F3 polysaccharides

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry
  • Molecular Medicine
  • Molecular Biology
  • Clinical Biochemistry
  • Biochemistry

Cite this

Effect of Reishi polysaccharides on human stem/progenitor cells. / Chen, Wan Yu; Yang, Wen Bin; Wong, Chi Huey; Shih, Tzu-Bi.

In: Bioorganic and Medicinal Chemistry, Vol. 18, No. 24, 15.12.2010, p. 8583-8591.

Research output: Contribution to journalArticle

Chen, Wan Yu ; Yang, Wen Bin ; Wong, Chi Huey ; Shih, Tzu-Bi. / Effect of Reishi polysaccharides on human stem/progenitor cells. In: Bioorganic and Medicinal Chemistry. 2010 ; Vol. 18, No. 24. pp. 8583-8591.
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