Effect of probiotics Lactobacillus acidophilus on Citrobacter rodentium colitis: The role of dendritic cells

Chien Chang Chen, Cheng Hsun Chiu, Tzou Yien Lin, Hai Ning Shi, W. Allan Walker

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Modulation of the intestinal immune response early in life by administration of probiotic bacteria may be an effective strategy for preventing or attenuating infectious diarrhea. We preinoculated the mice early in life with the probiotic bacteria Lactobacillus acidophilus NCFM (La) at age 2 wk. Dendritic cells (DCs) were collected and purified from mesenteric lymph nodes (MLN) and spleens of the BalbC/ByJ mice. DC isolation and adoptive transfer was used to examine the function of probiotics. We demonstrated that when mice were adoptively transferred with La-primed DCs (t-LaDC) instead of oral consumption with La, there was a similar effect on fecal bacteria counts, IgA levels, and colonic histopathology, as well as cytokine levels in MLN when there was intestinal bacterial infection. The above findings suggest that DCs play a key role in probiotics attenuating Citrobacter rodentium (Cr) colitis. Moreover, the location of La-primed DC hints that there is interaction of DCs and T cells in the digestive system of the host. Up-regulated expression of a surface marker on DCs indicated that inoculation with probiotics will stimulate the function of DCs, thereby further increasing immune response triggered by DC.

Original languageEnglish
Pages (from-to)169-175
Number of pages7
JournalPediatric Research
Volume65
Issue number2
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Fingerprint

Citrobacter rodentium
Lactobacillus acidophilus
Probiotics
Colitis
Dendritic Cells
Bacteria
Lymph Nodes
Digestive System
Adoptive Transfer
Cell Separation
Bacterial Infections
Immunoglobulin A
Diarrhea
Spleen

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Effect of probiotics Lactobacillus acidophilus on Citrobacter rodentium colitis : The role of dendritic cells. / Chen, Chien Chang; Chiu, Cheng Hsun; Lin, Tzou Yien; Shi, Hai Ning; Walker, W. Allan.

In: Pediatric Research, Vol. 65, No. 2, 02.2009, p. 169-175.

Research output: Contribution to journalArticle

Chen, Chien Chang ; Chiu, Cheng Hsun ; Lin, Tzou Yien ; Shi, Hai Ning ; Walker, W. Allan. / Effect of probiotics Lactobacillus acidophilus on Citrobacter rodentium colitis : The role of dendritic cells. In: Pediatric Research. 2009 ; Vol. 65, No. 2. pp. 169-175.
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