Effect of platelet-rich plasma mixed with a polymeric bone filling material on sinus floor augmentation in rabbits

Ching Li Tseng, Po Chih Hsu, Lin Gwei Wei, Te Hsing Wu, Li Han Shen, Guang Wei Chang, Hsu Wei Fang, Jui Che Tsai, Yi Ching Shen, Chi Chang Wu, Tim Mo Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Sinus augmentation is a common approach for patients with severe alveolar ridge atrophy but an ideal material to increase the bone volume for dental implantation is still needed. The present study evaluated the effect of a newly developed polymeric bone-filling powder (formed from acrylic acid and N-isopropylacrylamide, ANa) mixed with platelet-rich plasma (PRP) for bone generation in a rabbit model of sinus augmentation. A total of 12 New Zealand White rabbits were randomly divided into three groups based on filling material. All animals underwent a bilateral maxillary sinus augmentation. PRP was prepared using an automatic separation system to obtain a high platelet concentration. ANa powder was individually mixed with phosphate-buffered saline (PBS) or PRP for sinus floor elevation. The left maxillary sinus received the ANa/PBS filling, whereas the right maxillary sinus received a mixture of ANa/PRP gel. Equal volumes of filling material were inserted in each maxillary sinus. Thus, defects with no implantation served as controls. Animals were sacrificed at 4 and 12 weeks, and then all specimens were harvested for micro-computed tomography (micro-CT) and histological analysis. On micro-CT evaluation, ANa/PRP significantly increased the bone volume in maxillary sinus augmentation relative to the negative control and ANa/PBS after 12 weeks. New bone areas with osteocytes and osteon formation were found in all three groups at 12 weeks post-implantation. This study confirms that ANa mixed with PRP can effectively increase the bone volume in the sinus cavity. This benefit for sinus augmentation may increase the success of dental implantation because of the formation of a thin layer of alveolar bone.

Original languageEnglish
Article number1340004
JournalBiomedical Engineering - Applications, Basis and Communications
Volume25
Issue number5
DOIs
Publication statusPublished - Oct 2013

Fingerprint

Sinus Floor Augmentation
Platelet-Rich Plasma
Platelets
Maxillary Sinus
Bone
Rabbits
Plasmas
Bone and Bones
Dental Implantation
Phosphates
Powders
Tomography
Animals
Haversian System
Alveolar Process
Osteocytes
Acrylics
Atrophy
Blood Platelets
Gels

Keywords

  • Bone
  • Micro-computed tomography
  • Platelet-rich plasma
  • Polymer
  • Sinus augmentation

ASJC Scopus subject areas

  • Biophysics
  • Biomedical Engineering
  • Bioengineering

Cite this

Effect of platelet-rich plasma mixed with a polymeric bone filling material on sinus floor augmentation in rabbits. / Tseng, Ching Li; Hsu, Po Chih; Wei, Lin Gwei; Wu, Te Hsing; Shen, Li Han; Chang, Guang Wei; Fang, Hsu Wei; Tsai, Jui Che; Shen, Yi Ching; Wu, Chi Chang; Chen, Tim Mo.

In: Biomedical Engineering - Applications, Basis and Communications, Vol. 25, No. 5, 1340004, 10.2013.

Research output: Contribution to journalArticle

Tseng, Ching Li ; Hsu, Po Chih ; Wei, Lin Gwei ; Wu, Te Hsing ; Shen, Li Han ; Chang, Guang Wei ; Fang, Hsu Wei ; Tsai, Jui Che ; Shen, Yi Ching ; Wu, Chi Chang ; Chen, Tim Mo. / Effect of platelet-rich plasma mixed with a polymeric bone filling material on sinus floor augmentation in rabbits. In: Biomedical Engineering - Applications, Basis and Communications. 2013 ; Vol. 25, No. 5.
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AU - Chang, Guang Wei

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AB - Sinus augmentation is a common approach for patients with severe alveolar ridge atrophy but an ideal material to increase the bone volume for dental implantation is still needed. The present study evaluated the effect of a newly developed polymeric bone-filling powder (formed from acrylic acid and N-isopropylacrylamide, ANa) mixed with platelet-rich plasma (PRP) for bone generation in a rabbit model of sinus augmentation. A total of 12 New Zealand White rabbits were randomly divided into three groups based on filling material. All animals underwent a bilateral maxillary sinus augmentation. PRP was prepared using an automatic separation system to obtain a high platelet concentration. ANa powder was individually mixed with phosphate-buffered saline (PBS) or PRP for sinus floor elevation. The left maxillary sinus received the ANa/PBS filling, whereas the right maxillary sinus received a mixture of ANa/PRP gel. Equal volumes of filling material were inserted in each maxillary sinus. Thus, defects with no implantation served as controls. Animals were sacrificed at 4 and 12 weeks, and then all specimens were harvested for micro-computed tomography (micro-CT) and histological analysis. On micro-CT evaluation, ANa/PRP significantly increased the bone volume in maxillary sinus augmentation relative to the negative control and ANa/PBS after 12 weeks. New bone areas with osteocytes and osteon formation were found in all three groups at 12 weeks post-implantation. This study confirms that ANa mixed with PRP can effectively increase the bone volume in the sinus cavity. This benefit for sinus augmentation may increase the success of dental implantation because of the formation of a thin layer of alveolar bone.

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