Abstract
To clarify the effect of peritoneal fluids (PF) of women with various stages of endometriosis on sperm motility, we utilized computer-aided sperm analysis (CASA) to analyze sperm movement characteristics. PF was collected in the early follicular phase (days 4-8) of the menstrual cycle from 48 women undergoing diagnostic laparoscopy. Only sperm samples having normal sperm parameters were chosen for study. Swim-up separation was performed for 1 h at 37°C. Sperm suspension was mixed at a ratio of 1:1 with each of the following four groups: group 1, human tubal fluid (HTF) with 10% fetal bovine serum (control), group 2, normal PF (n = 16); group 3, minimal or mild endometriosis PF (n = 16); and group 4, moderate or severe endometriosis PF (n = 16). The mixtures were analyzed at 0, 1, 3, 6, and 24 h of co-incubation using the CASA system. At the end of the 24 h incubation, Supravital staining of sperm was done to check the viability of sperm in each group. Only time (F = 126.6, p < .001) has a significant effect on sperm motion parameters. At 6 h, sperm velocity (mean curvilinear velocity and mean straight line velocity) of the PF groups was significantly greater than that of the control, but there was no significant difference between each PF group. At 24 h, the PF groups maintained 50% of initial sperm viability, compared with 13% of initial viability in the control group (p < .001). There was no adverse effect of PF in patients with endometriosis on sperm motion parameters.
| Original language | English |
|---|---|
| Pages (from-to) | 49-55 |
| Number of pages | 7 |
| Journal | Archives of Andrology |
| Volume | 38 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Jan 1 1997 |
| Externally published | Yes |
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Keywords
- CASA
- endometriosis
- peritoneal fluid
- sperm toxicity
- women
ASJC Scopus subject areas
- Endocrinology
Cite this
Effect of peritoneal fluid on sperm motility parameters in women with endometriosis. / Chen, C. D.; Wu, M. Y.; Chao, K. H.; Chen, H. F.; Chen, S. U.; Ho, H. N.; Huang, S. C.; Yang, Y. S.
In: Archives of Andrology, Vol. 38, No. 1, 01.01.1997, p. 49-55.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of peritoneal fluid on sperm motility parameters in women with endometriosis
AU - Chen, C. D.
AU - Wu, M. Y.
AU - Chao, K. H.
AU - Chen, H. F.
AU - Chen, S. U.
AU - Ho, H. N.
AU - Huang, S. C.
AU - Yang, Y. S.
PY - 1997/1/1
Y1 - 1997/1/1
N2 - To clarify the effect of peritoneal fluids (PF) of women with various stages of endometriosis on sperm motility, we utilized computer-aided sperm analysis (CASA) to analyze sperm movement characteristics. PF was collected in the early follicular phase (days 4-8) of the menstrual cycle from 48 women undergoing diagnostic laparoscopy. Only sperm samples having normal sperm parameters were chosen for study. Swim-up separation was performed for 1 h at 37°C. Sperm suspension was mixed at a ratio of 1:1 with each of the following four groups: group 1, human tubal fluid (HTF) with 10% fetal bovine serum (control), group 2, normal PF (n = 16); group 3, minimal or mild endometriosis PF (n = 16); and group 4, moderate or severe endometriosis PF (n = 16). The mixtures were analyzed at 0, 1, 3, 6, and 24 h of co-incubation using the CASA system. At the end of the 24 h incubation, Supravital staining of sperm was done to check the viability of sperm in each group. Only time (F = 126.6, p < .001) has a significant effect on sperm motion parameters. At 6 h, sperm velocity (mean curvilinear velocity and mean straight line velocity) of the PF groups was significantly greater than that of the control, but there was no significant difference between each PF group. At 24 h, the PF groups maintained 50% of initial sperm viability, compared with 13% of initial viability in the control group (p < .001). There was no adverse effect of PF in patients with endometriosis on sperm motion parameters.
AB - To clarify the effect of peritoneal fluids (PF) of women with various stages of endometriosis on sperm motility, we utilized computer-aided sperm analysis (CASA) to analyze sperm movement characteristics. PF was collected in the early follicular phase (days 4-8) of the menstrual cycle from 48 women undergoing diagnostic laparoscopy. Only sperm samples having normal sperm parameters were chosen for study. Swim-up separation was performed for 1 h at 37°C. Sperm suspension was mixed at a ratio of 1:1 with each of the following four groups: group 1, human tubal fluid (HTF) with 10% fetal bovine serum (control), group 2, normal PF (n = 16); group 3, minimal or mild endometriosis PF (n = 16); and group 4, moderate or severe endometriosis PF (n = 16). The mixtures were analyzed at 0, 1, 3, 6, and 24 h of co-incubation using the CASA system. At the end of the 24 h incubation, Supravital staining of sperm was done to check the viability of sperm in each group. Only time (F = 126.6, p < .001) has a significant effect on sperm motion parameters. At 6 h, sperm velocity (mean curvilinear velocity and mean straight line velocity) of the PF groups was significantly greater than that of the control, but there was no significant difference between each PF group. At 24 h, the PF groups maintained 50% of initial sperm viability, compared with 13% of initial viability in the control group (p < .001). There was no adverse effect of PF in patients with endometriosis on sperm motion parameters.
KW - CASA
KW - endometriosis
KW - peritoneal fluid
KW - sperm toxicity
KW - women
UR - http://www.scopus.com/inward/record.url?scp=79961231261&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79961231261&partnerID=8YFLogxK
U2 - 10.3109/01485019708988531
DO - 10.3109/01485019708988531
M3 - Article
C2 - 9017122
AN - SCOPUS:79961231261
VL - 38
SP - 49
EP - 55
JO - Systems Biology in Reproductive Medicine
JF - Systems Biology in Reproductive Medicine
SN - 1939-6376
IS - 1
ER -