Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer

Jane C J Chao, Kuo Yu Liu, Sheng-Hsuan Chen, Chia Lang Fang, Chih Wei Tsao

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Aim: To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer. Methods: Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 μg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA. Results: The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44±0.54 mg/g vs 1.45±0.52 mg/g mucosa, P

Original languageEnglish
Pages (from-to)2261-2265
Number of pages5
JournalWorld Journal of Gastroenterology
Volume9
Issue number10
Publication statusPublished - Oct 2003

Fingerprint

Duodenal Ulcer
Epidermal Growth Factor
Mucous Membrane
Hematoxylin
Eosine Yellowish-(YS)
Acetic Acid
Drinking Water
Nucleic Acids
Sprague Dawley Rats
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Control Groups

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer. / Chao, Jane C J; Liu, Kuo Yu; Chen, Sheng-Hsuan; Fang, Chia Lang; Tsao, Chih Wei.

In: World Journal of Gastroenterology, Vol. 9, No. 10, 10.2003, p. 2261-2265.

Research output: Contribution to journalArticle

Chao, Jane C J ; Liu, Kuo Yu ; Chen, Sheng-Hsuan ; Fang, Chia Lang ; Tsao, Chih Wei. / Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer. In: World Journal of Gastroenterology. 2003 ; Vol. 9, No. 10. pp. 2261-2265.
@article{deed5b69878a4662b75a29dd33b71c00,
title = "Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer",
abstract = "Aim: To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer. Methods: Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 μg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA. Results: The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44±0.54 mg/g vs 1.45±0.52 mg/g mucosa, P",
author = "Chao, {Jane C J} and Liu, {Kuo Yu} and Sheng-Hsuan Chen and Fang, {Chia Lang} and Tsao, {Chih Wei}",
year = "2003",
month = "10",
language = "English",
volume = "9",
pages = "2261--2265",
journal = "World Journal of Gastroenterology",
issn = "1007-9327",
publisher = "WJG Press",
number = "10",

}

TY - JOUR

T1 - Effect of oral epidermal growth factor on mucosal healing in rats with duodenal ulcer

AU - Chao, Jane C J

AU - Liu, Kuo Yu

AU - Chen, Sheng-Hsuan

AU - Fang, Chia Lang

AU - Tsao, Chih Wei

PY - 2003/10

Y1 - 2003/10

N2 - Aim: To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer. Methods: Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 μg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA. Results: The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44±0.54 mg/g vs 1.45±0.52 mg/g mucosa, P

AB - Aim: To investigate the effect of epidermal growth factor (EGF) on mucosal healing in rats with duodenal ulcer. Methods: Male Sprague-Dawley rats were randomly divided into sham operation without EGF, sham operation with EGF, duodenal ulcer without EGF, or duodenal ulcer with EGF groups. Additionally, normal rats without operation served as the control group. Duodenal ulcer was induced in rats by 300 mL/L acetic acid. Rats with EGF were orally administered at a dose of 60 μg/kg/day in drinking water on the next day of operation (day 1). Healing of duodenal ulcer was detected by haematoxylin and eosin staining. Cell growth of damaged mucosa was determined by the contents of nucleic acids and proteins. The level of EGF in duodenal mucosa was measured by ELISA. Results: The pathological results showed that duodenal ulcer rats with EGF improved mucosal healing compared with those without EGF after day 5. Duodenal ulcer rats with EGF significantly increased duodenal DNA content compared with those without EGF on day 15 (6.44±0.54 mg/g vs 1.45±0.52 mg/g mucosa, P

UR - http://www.scopus.com/inward/record.url?scp=0142213432&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0142213432&partnerID=8YFLogxK

M3 - Article

C2 - 14562389

AN - SCOPUS:0142213432

VL - 9

SP - 2261

EP - 2265

JO - World Journal of Gastroenterology

JF - World Journal of Gastroenterology

SN - 1007-9327

IS - 10

ER -