Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice

Roles of nitric oxide and prostaglandin E2

Yu Chih Liang, Hung Jung Liu, Sheng-Hsuan Chen, Chun Chin Chen, Liang Shung Chou, Li-Hsueh Tsai

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Aim: To investigate the effect of lipopolysaccharide (LPS) the diarrheogenic activity, gastrointestinal transit (GIT), and intestinal fluid content and the possible role of nitric oxide (NO) and prostaglandin E2 (PGE2) in gastrointestinal functions of endotoxin-treated mice. Methods: Diarrheogic activity, GIT, and intestinal fluid content as well as nitric oxide and PGE2 products were measured after intraperitoneal administration of LPS in mice. Results: LPS dose-dependently accumulated abundant fluid into the small intestine, induced diarrhea, but decreased the GIT. Both nitric oxide and PGE2 were found to increase in LPS-treated mice. Western blot analysis indicated that LPS significantly induced the protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 in mice intestines. Pretreatment with NG-nitro-L-arginine-methyl ester (L-NAME, a non-selective NOS inhibitor) or indomethacin (an inhibitor of prostaglandin synthesis) significantly attenuated the effects of LPS on the diarrheogenic activity and intestine content, but reversed the GIT. Conclusion: The present study suggests that the pathogenesis of LPS treatment may mediate the stimulatory effect of LPS on nitric oxide and PGE2 production and NO/prostaglandin pathway may play an important role on gastrointestinal function.

Original languageEnglish
Pages (from-to)357-361
Number of pages5
JournalWorld Journal of Gastroenterology
Volume11
Issue number3
Publication statusPublished - Jan 21 2005

Fingerprint

Gastrointestinal Transit
Dinoprostone
Lipopolysaccharides
Diarrhea
Nitric Oxide
Gastrointestinal Contents
NG-Nitroarginine Methyl Ester
Intestines
Prostaglandin Antagonists
Nitric Oxide Synthase Type II
Endotoxins
Indomethacin
Small Intestine
Prostaglandins
Western Blotting

Keywords

  • Diarrhea
  • Gastrointestinal transit
  • Lipopolysaccharide
  • Nitric oxide
  • Prostaglandin E2

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice : Roles of nitric oxide and prostaglandin E2. / Liang, Yu Chih; Liu, Hung Jung; Chen, Sheng-Hsuan; Chen, Chun Chin; Chou, Liang Shung; Tsai, Li-Hsueh.

In: World Journal of Gastroenterology, Vol. 11, No. 3, 21.01.2005, p. 357-361.

Research output: Contribution to journalArticle

Liang, Yu Chih ; Liu, Hung Jung ; Chen, Sheng-Hsuan ; Chen, Chun Chin ; Chou, Liang Shung ; Tsai, Li-Hsueh. / Effect of lipopolysaccharide on diarrhea and gastrointestinal transit in mice : Roles of nitric oxide and prostaglandin E2. In: World Journal of Gastroenterology. 2005 ; Vol. 11, No. 3. pp. 357-361.
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