Effect of hyperbaric oxygenation on intervertebral disc degeneration

An in vivo study with sprague-dawley rats

I. Chun Wang, Hsien Tao Liu, Chung Ming Yu, Shu Wen Whu, Song Shu Lin, Chun I. Su, Chih Hwa Chen, Wen Jer Chen

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

STUDY DESIGN.: An in vivo study was conducted to test the effect of hyperbaric oxygenation (HBO) on intervertebral disc degeneration in Sprague-Dawley rats. OBJECTIVE.: To observe the changes in intervertebral disc height and levels of glycosaminoglycan, collagen, interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and inducible nitric oxide synthase (iNOS) in degenerated intervertebral discs after HBO therapy. SUMMARY OF BACKGROUND DATA.: Although the involvement of IL-1β, PGE-2, NO, and low O2 concentration has been demonstrated in intervertebral disc degeneration, the actual mechanism is not clear. It has been reported that HBO influences changes in IL-1β, PGE-2, NO, and O2 concentration. Previously, a study demonstrated an in vitro positive effect of HBO on the human nucleus pulposus. Thus, an in vivo study in animals was necessary. METHODS.: Twelve Sprague-Dawley rats were each injected with chondroitinase ABC in 2 proximal intervertebral discs of the tail. After treating with 100% oxygen at 2.5 atmospheres 2 hours per days for 10 days, the change in disc height was determined by radiography. The amounts of PGE-2, iNOS, glycosaminoglycan, and total collagen in the intervertebral disc were quantified by enzyme-linked immunosorbent assay. Tissue morphology and the distribution of glycosaminoglycan, IL-1β, and iNOS in the intervertebral disc were assessed by histology and immunohistochemistry. The area of IL-1β in the intervertebral discs was quantified using image analysis software. RESULTS.: HBO therapy stopped the decrease in intervertebral disc height, caused an increase in the amount of glycosaminoglycan, and inhibited IL-1β, PGE-2, and iNOS production. CONCLUSION.: HBO provides a potential treatment modality for intervertebral disc degeneration.

Original languageEnglish
JournalSpine
Volume38
Issue number3
DOIs
Publication statusPublished - Feb 1 2013
Externally publishedYes

Fingerprint

Intervertebral Disc Degeneration
Hyperbaric Oxygenation
Intervertebral Disc
Sprague Dawley Rats
Interleukin-1
Nitric Oxide Synthase Type II
Prostaglandins E
Glycosaminoglycans
Collagen
Chondroitin ABC Lyase
Tissue Distribution
Atmosphere
Dinoprostone
Radiography
Tail
Histology
Therapeutics
Software
Enzyme-Linked Immunosorbent Assay
Immunohistochemistry

Keywords

  • hyperbaric oxygenation
  • in vivo
  • intervertebral disc degeneration
  • Sprague-Dawley rat model

ASJC Scopus subject areas

  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

Effect of hyperbaric oxygenation on intervertebral disc degeneration : An in vivo study with sprague-dawley rats. / Wang, I. Chun; Liu, Hsien Tao; Yu, Chung Ming; Whu, Shu Wen; Lin, Song Shu; Su, Chun I.; Chen, Chih Hwa; Chen, Wen Jer.

In: Spine, Vol. 38, No. 3, 01.02.2013.

Research output: Contribution to journalArticle

Wang, I. Chun ; Liu, Hsien Tao ; Yu, Chung Ming ; Whu, Shu Wen ; Lin, Song Shu ; Su, Chun I. ; Chen, Chih Hwa ; Chen, Wen Jer. / Effect of hyperbaric oxygenation on intervertebral disc degeneration : An in vivo study with sprague-dawley rats. In: Spine. 2013 ; Vol. 38, No. 3.
@article{8821aa936ca54750a8ef3fc9cd113c74,
title = "Effect of hyperbaric oxygenation on intervertebral disc degeneration: An in vivo study with sprague-dawley rats",
abstract = "STUDY DESIGN.: An in vivo study was conducted to test the effect of hyperbaric oxygenation (HBO) on intervertebral disc degeneration in Sprague-Dawley rats. OBJECTIVE.: To observe the changes in intervertebral disc height and levels of glycosaminoglycan, collagen, interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and inducible nitric oxide synthase (iNOS) in degenerated intervertebral discs after HBO therapy. SUMMARY OF BACKGROUND DATA.: Although the involvement of IL-1β, PGE-2, NO, and low O2 concentration has been demonstrated in intervertebral disc degeneration, the actual mechanism is not clear. It has been reported that HBO influences changes in IL-1β, PGE-2, NO, and O2 concentration. Previously, a study demonstrated an in vitro positive effect of HBO on the human nucleus pulposus. Thus, an in vivo study in animals was necessary. METHODS.: Twelve Sprague-Dawley rats were each injected with chondroitinase ABC in 2 proximal intervertebral discs of the tail. After treating with 100{\%} oxygen at 2.5 atmospheres 2 hours per days for 10 days, the change in disc height was determined by radiography. The amounts of PGE-2, iNOS, glycosaminoglycan, and total collagen in the intervertebral disc were quantified by enzyme-linked immunosorbent assay. Tissue morphology and the distribution of glycosaminoglycan, IL-1β, and iNOS in the intervertebral disc were assessed by histology and immunohistochemistry. The area of IL-1β in the intervertebral discs was quantified using image analysis software. RESULTS.: HBO therapy stopped the decrease in intervertebral disc height, caused an increase in the amount of glycosaminoglycan, and inhibited IL-1β, PGE-2, and iNOS production. CONCLUSION.: HBO provides a potential treatment modality for intervertebral disc degeneration.",
keywords = "hyperbaric oxygenation, in vivo, intervertebral disc degeneration, Sprague-Dawley rat model",
author = "Wang, {I. Chun} and Liu, {Hsien Tao} and Yu, {Chung Ming} and Whu, {Shu Wen} and Lin, {Song Shu} and Su, {Chun I.} and Chen, {Chih Hwa} and Chen, {Wen Jer}",
year = "2013",
month = "2",
day = "1",
doi = "10.1097/BRS.0b013e31827bf6bf",
language = "English",
volume = "38",
journal = "Spine",
issn = "1528-1159",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Effect of hyperbaric oxygenation on intervertebral disc degeneration

T2 - An in vivo study with sprague-dawley rats

AU - Wang, I. Chun

AU - Liu, Hsien Tao

AU - Yu, Chung Ming

AU - Whu, Shu Wen

AU - Lin, Song Shu

AU - Su, Chun I.

AU - Chen, Chih Hwa

AU - Chen, Wen Jer

PY - 2013/2/1

Y1 - 2013/2/1

N2 - STUDY DESIGN.: An in vivo study was conducted to test the effect of hyperbaric oxygenation (HBO) on intervertebral disc degeneration in Sprague-Dawley rats. OBJECTIVE.: To observe the changes in intervertebral disc height and levels of glycosaminoglycan, collagen, interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and inducible nitric oxide synthase (iNOS) in degenerated intervertebral discs after HBO therapy. SUMMARY OF BACKGROUND DATA.: Although the involvement of IL-1β, PGE-2, NO, and low O2 concentration has been demonstrated in intervertebral disc degeneration, the actual mechanism is not clear. It has been reported that HBO influences changes in IL-1β, PGE-2, NO, and O2 concentration. Previously, a study demonstrated an in vitro positive effect of HBO on the human nucleus pulposus. Thus, an in vivo study in animals was necessary. METHODS.: Twelve Sprague-Dawley rats were each injected with chondroitinase ABC in 2 proximal intervertebral discs of the tail. After treating with 100% oxygen at 2.5 atmospheres 2 hours per days for 10 days, the change in disc height was determined by radiography. The amounts of PGE-2, iNOS, glycosaminoglycan, and total collagen in the intervertebral disc were quantified by enzyme-linked immunosorbent assay. Tissue morphology and the distribution of glycosaminoglycan, IL-1β, and iNOS in the intervertebral disc were assessed by histology and immunohistochemistry. The area of IL-1β in the intervertebral discs was quantified using image analysis software. RESULTS.: HBO therapy stopped the decrease in intervertebral disc height, caused an increase in the amount of glycosaminoglycan, and inhibited IL-1β, PGE-2, and iNOS production. CONCLUSION.: HBO provides a potential treatment modality for intervertebral disc degeneration.

AB - STUDY DESIGN.: An in vivo study was conducted to test the effect of hyperbaric oxygenation (HBO) on intervertebral disc degeneration in Sprague-Dawley rats. OBJECTIVE.: To observe the changes in intervertebral disc height and levels of glycosaminoglycan, collagen, interleukin-1β (IL-1β), prostaglandin E2 (PGE2), and inducible nitric oxide synthase (iNOS) in degenerated intervertebral discs after HBO therapy. SUMMARY OF BACKGROUND DATA.: Although the involvement of IL-1β, PGE-2, NO, and low O2 concentration has been demonstrated in intervertebral disc degeneration, the actual mechanism is not clear. It has been reported that HBO influences changes in IL-1β, PGE-2, NO, and O2 concentration. Previously, a study demonstrated an in vitro positive effect of HBO on the human nucleus pulposus. Thus, an in vivo study in animals was necessary. METHODS.: Twelve Sprague-Dawley rats were each injected with chondroitinase ABC in 2 proximal intervertebral discs of the tail. After treating with 100% oxygen at 2.5 atmospheres 2 hours per days for 10 days, the change in disc height was determined by radiography. The amounts of PGE-2, iNOS, glycosaminoglycan, and total collagen in the intervertebral disc were quantified by enzyme-linked immunosorbent assay. Tissue morphology and the distribution of glycosaminoglycan, IL-1β, and iNOS in the intervertebral disc were assessed by histology and immunohistochemistry. The area of IL-1β in the intervertebral discs was quantified using image analysis software. RESULTS.: HBO therapy stopped the decrease in intervertebral disc height, caused an increase in the amount of glycosaminoglycan, and inhibited IL-1β, PGE-2, and iNOS production. CONCLUSION.: HBO provides a potential treatment modality for intervertebral disc degeneration.

KW - hyperbaric oxygenation

KW - in vivo

KW - intervertebral disc degeneration

KW - Sprague-Dawley rat model

UR - http://www.scopus.com/inward/record.url?scp=84873406630&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84873406630&partnerID=8YFLogxK

U2 - 10.1097/BRS.0b013e31827bf6bf

DO - 10.1097/BRS.0b013e31827bf6bf

M3 - Article

VL - 38

JO - Spine

JF - Spine

SN - 1528-1159

IS - 3

ER -