Effect of hyperbaric oxygenation on intervertebral disc degeneration: An in vitro study with human lumbar nucleus pulposus

I. Chun Wang, Steve W N Ueng, Song Shu Lin, Chi Chien Niu, Li Jen Yuan, Chun I. Su, Chih Hwa Chen, Wen Jer Chen

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Study Design.: An in vitro study with degenerated human lumbar intervertebral disc specimens cultured under hyperbaric oxygenation (HBO). Objective.: To observe the changes in interleukin (IL)-1β, prostaglandin (PG)-E2, nitric oxide (NO), cell growth, and apoptosis of the human nucleus pulposus cell (NPC) after HBO. Summary of Background Data.: Intervertebral disc degeneration has been demonstrated as related to IL-1β, PG-E2, NO, and O2 concentration but the actual mechanism is not clear. HBO also has also been reported in the literature to influence changes in IL-1β, prostaglandin E2, NO, and O2 concentration. However, the direct effect of HBO on the disc cells has not been previously reported. Methods.: We collected 12 human lumbar degenerated disc specimens and evaluated the effects of HBO on the cultured NPCs. The amounts of IL-1β, PG-E2, and NO in the conditioned medium were quantified by enzyme-linked immunosorbent assay and high performance liquid chromatography. Cell growth was measured by increase in cell number. Cell viability and proteoglycan content were evaluated by histologic study using safranin O staining. In situ analysis of apoptosis was performed using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) staining. Results.: Our data indicated that HBO treatment inhibited IL-1β, PG-E2, and NO production but increased cell number and matrix synthesis of cultured NPCs. TUNEL staining showed that HBO treatment suppressed the apoptosis of cultured NPCs. Conclusion.: HBO provides a potential treatment modality for disc degeneration.

Original languageEnglish
Pages (from-to)1925-1931
Number of pages7
JournalSpine
Volume36
Issue number23
DOIs
Publication statusPublished - Nov 1 2011
Externally publishedYes

Fingerprint

Intervertebral Disc Degeneration
Hyperbaric Oxygenation
Interleukin-1
Dinoprostone
Nitric Oxide
Apoptosis
Staining and Labeling
Cell Count
DNA Nucleotidylexotransferase
Nucleus Pulposus
In Vitro Techniques
Intervertebral Disc
Proteoglycans
Conditioned Culture Medium
Growth
Transferases
Cell Survival
Therapeutics
Enzyme-Linked Immunosorbent Assay
High Pressure Liquid Chromatography

Keywords

  • apoptosis
  • disc degeneration
  • hyperbaric oxygenation

ASJC Scopus subject areas

  • Clinical Neurology
  • Orthopedics and Sports Medicine

Cite this

Wang, I. C., Ueng, S. W. N., Lin, S. S., Niu, C. C., Yuan, L. J., Su, C. I., ... Chen, W. J. (2011). Effect of hyperbaric oxygenation on intervertebral disc degeneration: An in vitro study with human lumbar nucleus pulposus. Spine, 36(23), 1925-1931. https://doi.org/10.1097/BRS.0b013e3181feebde

Effect of hyperbaric oxygenation on intervertebral disc degeneration : An in vitro study with human lumbar nucleus pulposus. / Wang, I. Chun; Ueng, Steve W N; Lin, Song Shu; Niu, Chi Chien; Yuan, Li Jen; Su, Chun I.; Chen, Chih Hwa; Chen, Wen Jer.

In: Spine, Vol. 36, No. 23, 01.11.2011, p. 1925-1931.

Research output: Contribution to journalArticle

Wang, I. Chun ; Ueng, Steve W N ; Lin, Song Shu ; Niu, Chi Chien ; Yuan, Li Jen ; Su, Chun I. ; Chen, Chih Hwa ; Chen, Wen Jer. / Effect of hyperbaric oxygenation on intervertebral disc degeneration : An in vitro study with human lumbar nucleus pulposus. In: Spine. 2011 ; Vol. 36, No. 23. pp. 1925-1931.
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