Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines

Gi Ming Lai, Robert F. Ozols, Robert C. Young, Thomas C. Hamilton

Research output: Contribution to journalArticle

154 Citations (Scopus)

Abstract

We have studied the effect of glutathione reduction by buthionine sulf-oximine (BSO), a specific inhibitor ofλ -glutamyl cysteine synthetase, on DNA repair after cisplatin damage in an ovarian cancer cell line with invitro induced resistance to cisplatin. In addition, we have examined the effect of aphidicolin, a specific inhibitor of DNA polymerase a, in combination with BSO on cisplatin-associated DNA repair. BSO treatment was found topartially inhibit DNA repair, and the addition of aphidicolin caused nearly a100% inhibition in DNA repair activity. Treatment of cells with glutathioneester after BSO resulted in complete recovery of DNA repair activity or par-tial recovery if aphidicolin was present. The significance of these results to the chemosensitizing effects of BSO medi-ated glutathione reduction is discussed. [J Natl Cancer Inst 81:535-539, 1989].

Original languageEnglish
Pages (from-to)535-539
Number of pages5
JournalJournal of the National Cancer Institute
Volume81
Issue number7
DOIs
Publication statusPublished - Apr 5 1989
Externally publishedYes

Fingerprint

Cisplatin
Ovarian Cancer
Glutathione
DNA Repair
Ovarian Neoplasms
Repair
Aphidicolin
DNA
Cells
Cell Line
Line
Cell
Inhibitor
Recovery
Nucleic Acid Synthesis Inhibitors
Ligases
Cysteine
Cancer
Damage
Human

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Oncology
  • Cancer Research

Cite this

Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines. / Lai, Gi Ming; Ozols, Robert F.; Young, Robert C.; Hamilton, Thomas C.

In: Journal of the National Cancer Institute, Vol. 81, No. 7, 05.04.1989, p. 535-539.

Research output: Contribution to journalArticle

Lai, Gi Ming ; Ozols, Robert F. ; Young, Robert C. ; Hamilton, Thomas C. / Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines. In: Journal of the National Cancer Institute. 1989 ; Vol. 81, No. 7. pp. 535-539.
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