Effect of glutathione on DNA repair in cisplatin-resistant human ovarian cancer cell lines

Gi Ming Lai, Robert F. Ozols, Robert C. Young, Thomas C. Hamilton

Research output: Contribution to journalArticle

156 Citations (Scopus)

Abstract

We have studied the effect of glutathione reduction by buthionine sulf-oximine (BSO), a specific inhibitor ofλ -glutamyl cysteine synthetase, on DNA repair after cisplatin damage in an ovarian cancer cell line with invitro induced resistance to cisplatin. In addition, we have examined the effect of aphidicolin, a specific inhibitor of DNA polymerase a, in combination with BSO on cisplatin-associated DNA repair. BSO treatment was found topartially inhibit DNA repair, and the addition of aphidicolin caused nearly a100% inhibition in DNA repair activity. Treatment of cells with glutathioneester after BSO resulted in complete recovery of DNA repair activity or par-tial recovery if aphidicolin was present. The significance of these results to the chemosensitizing effects of BSO medi-ated glutathione reduction is discussed. [J Natl Cancer Inst 81:535-539, 1989].

Original languageEnglish
Pages (from-to)535-539
Number of pages5
JournalJournal of the National Cancer Institute
Volume81
Issue number7
DOIs
Publication statusPublished - Apr 5 1989
Externally publishedYes

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Oncology
  • Cancer Research

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