Abstract
The aim of this study was to investigate the effects of folate supplementation on mitochondrial mass and membrane potential of rat primary hepatocytes treated with oxidants. Rat primary hepatocytes were obtained by a two-step collagenase perfusion and incubation in medium containing various concentrations of folate (0-1000 μM). Cells were treated with homocysteine (Hcy), Cu ++, or menadione, and changes of mitochondrial mass as well as membrane potential were assessed by flow cytometry. After Hcy treatment for 24 h, mitochondrial mass and membrane potential had significantly decreased in rat primary hepatocytes. Folate supplementation could partially normalize the mitochondrial membrane potential of Hcy-treated cells, but did not affect changes in mitochondrial mass. Upon Cu ++ treatment, the mitochondrial membrane potential of rat primary hepatocytes was significantly decreased. The decrease in the mitochondrial membrane potential induced by copper ion, was ameliorated by folate supplementation. After menadione treatment, cells became morphologically deformed and began dying. Folate supplementation affect neither the mitochondrial mass nor membrane potential changes in menadione-treated cells. Taken together, folate supplementation has some protective effects on Cu ++, and Hcy-induced changes in the mitochondrial membrane potential, but failed to affect mitochondrial mass.
| Original language | English |
|---|---|
| Pages (from-to) | 38-47 |
| Number of pages | 10 |
| Journal | Nutritional Sciences Journal |
| Volume | 29 |
| Issue number | 1 |
| Publication status | Published - Mar 2004 |
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Keywords
- Folate supplementation
- Mitochondrial mass
- Mitochondrial membrane potential
- Oxidants
- Rat primary hepatocytes
ASJC Scopus subject areas
- Medicine (miscellaneous)
- Food Science
Cite this
Effect of folate supplementation on mitochondrial mass and membrane potential of rat primary hepatocytes treated with oxidants. / Lu, Hsin Te; Lii, Chong Kuei; Cheng, Hsing Hsien; Huang, Rwei Fen S.
In: Nutritional Sciences Journal, Vol. 29, No. 1, 03.2004, p. 38-47.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Effect of folate supplementation on mitochondrial mass and membrane potential of rat primary hepatocytes treated with oxidants
AU - Lu, Hsin Te
AU - Lii, Chong Kuei
AU - Cheng, Hsing Hsien
AU - Huang, Rwei Fen S
PY - 2004/3
Y1 - 2004/3
N2 - The aim of this study was to investigate the effects of folate supplementation on mitochondrial mass and membrane potential of rat primary hepatocytes treated with oxidants. Rat primary hepatocytes were obtained by a two-step collagenase perfusion and incubation in medium containing various concentrations of folate (0-1000 μM). Cells were treated with homocysteine (Hcy), Cu ++, or menadione, and changes of mitochondrial mass as well as membrane potential were assessed by flow cytometry. After Hcy treatment for 24 h, mitochondrial mass and membrane potential had significantly decreased in rat primary hepatocytes. Folate supplementation could partially normalize the mitochondrial membrane potential of Hcy-treated cells, but did not affect changes in mitochondrial mass. Upon Cu ++ treatment, the mitochondrial membrane potential of rat primary hepatocytes was significantly decreased. The decrease in the mitochondrial membrane potential induced by copper ion, was ameliorated by folate supplementation. After menadione treatment, cells became morphologically deformed and began dying. Folate supplementation affect neither the mitochondrial mass nor membrane potential changes in menadione-treated cells. Taken together, folate supplementation has some protective effects on Cu ++, and Hcy-induced changes in the mitochondrial membrane potential, but failed to affect mitochondrial mass.
AB - The aim of this study was to investigate the effects of folate supplementation on mitochondrial mass and membrane potential of rat primary hepatocytes treated with oxidants. Rat primary hepatocytes were obtained by a two-step collagenase perfusion and incubation in medium containing various concentrations of folate (0-1000 μM). Cells were treated with homocysteine (Hcy), Cu ++, or menadione, and changes of mitochondrial mass as well as membrane potential were assessed by flow cytometry. After Hcy treatment for 24 h, mitochondrial mass and membrane potential had significantly decreased in rat primary hepatocytes. Folate supplementation could partially normalize the mitochondrial membrane potential of Hcy-treated cells, but did not affect changes in mitochondrial mass. Upon Cu ++ treatment, the mitochondrial membrane potential of rat primary hepatocytes was significantly decreased. The decrease in the mitochondrial membrane potential induced by copper ion, was ameliorated by folate supplementation. After menadione treatment, cells became morphologically deformed and began dying. Folate supplementation affect neither the mitochondrial mass nor membrane potential changes in menadione-treated cells. Taken together, folate supplementation has some protective effects on Cu ++, and Hcy-induced changes in the mitochondrial membrane potential, but failed to affect mitochondrial mass.
KW - Folate supplementation
KW - Mitochondrial mass
KW - Mitochondrial membrane potential
KW - Oxidants
KW - Rat primary hepatocytes
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UR - http://www.scopus.com/inward/citedby.url?scp=4644290764&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:4644290764
VL - 29
SP - 38
EP - 47
JO - Journal of the Chinese Nutrition Society
JF - Journal of the Chinese Nutrition Society
SN - 1011-6958
IS - 1
ER -