Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy

Pai Fu Wang, Allen W. Chiu, Yueh Min Lin, Ching Yuang Lin, Hung Jen Shih

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Background: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. Methods: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. Conclusions: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.

Original languageEnglish
Pages (from-to)304-309
Number of pages6
JournalInternational Journal of Surgery
Volume12
Issue number4
DOIs
Publication statusPublished - Jan 1 2014
Externally publishedYes

Fingerprint

dexon (fungicide)
Fibrin Tissue Adhesive
Nephrectomy
Kidney
Fibrin
Transforming Growth Factors
Reverse Transcription
Sprague Dawley Rats

Keywords

  • Dexon mesh
  • Fibrin sealant
  • Partial nephrectomy

ASJC Scopus subject areas

  • Surgery

Cite this

Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy. / Wang, Pai Fu; Chiu, Allen W.; Lin, Yueh Min; Lin, Ching Yuang; Shih, Hung Jen.

In: International Journal of Surgery, Vol. 12, No. 4, 01.01.2014, p. 304-309.

Research output: Contribution to journalArticle

Wang, Pai Fu ; Chiu, Allen W. ; Lin, Yueh Min ; Lin, Ching Yuang ; Shih, Hung Jen. / Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy. In: International Journal of Surgery. 2014 ; Vol. 12, No. 4. pp. 304-309.
@article{e12dfa6f9e624b99934509318f099cb4,
title = "Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy",
abstract = "Background: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. Methods: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. Conclusions: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.",
keywords = "Dexon mesh, Fibrin sealant, Partial nephrectomy",
author = "Wang, {Pai Fu} and Chiu, {Allen W.} and Lin, {Yueh Min} and Lin, {Ching Yuang} and Shih, {Hung Jen}",
year = "2014",
month = "1",
day = "1",
doi = "10.1016/j.ijsu.2014.01.024",
language = "English",
volume = "12",
pages = "304--309",
journal = "International Journal of Surgery",
issn = "1743-9191",
publisher = "Elsevier Science Publishers B.V.",
number = "4",

}

TY - JOUR

T1 - Effect of fibrin sealant aided with Dexon mesh for renal repair in a rat model of partial nephrectomy

AU - Wang, Pai Fu

AU - Chiu, Allen W.

AU - Lin, Yueh Min

AU - Lin, Ching Yuang

AU - Shih, Hung Jen

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Background: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. Methods: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. Conclusions: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.

AB - Background: To evaluate the clinical efficacy and histochemical impact of a new technique of renal repair using a fibrin sealant and Dexon mesh in rats. Methods: Ten groups of Sprague-Dawley (SD) rats underwent a bilateral partial nephrectomy 30, 21, 14, 7 to 1 days before sacrifice. Renal repair was accomplished by suturing on one side and using fibrin sealant and Dexon mesh on the opposite side. The time for renal reconstruction was recorded for each approach and compared. In addition to histological evaluations, the isolated renal tissue studies included immunohistochemical analysis, and semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Results: In comparison with suturing, renal repair using fibrin sealant and Dexon mesh was much faster. We demonstrated a significant attenuation of the initial inflammatory response in the fibrin-Dexon group. The specific alterations in transforming growth factor-β1 (Tgf-β1) mRNA expression were significantly lower in the fibrin-Dexon group. Conclusions: The fibrin sealant and Dexon mesh significantly simplified the procedure by reducing the time of renal reconstruction. This approach can diminish the fibrotic reaction and offers a response for renal repair similar to the suturing technique.

KW - Dexon mesh

KW - Fibrin sealant

KW - Partial nephrectomy

UR - http://www.scopus.com/inward/record.url?scp=84897374774&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897374774&partnerID=8YFLogxK

U2 - 10.1016/j.ijsu.2014.01.024

DO - 10.1016/j.ijsu.2014.01.024

M3 - Article

C2 - 24508571

AN - SCOPUS:84897374774

VL - 12

SP - 304

EP - 309

JO - International Journal of Surgery

JF - International Journal of Surgery

SN - 1743-9191

IS - 4

ER -