Effect of epidermal growth factor on leucine aminopeptidase in human colon (CACO-2) cells

J. C J Chao, W. H S Shieh, A. Tavlor

Research output: Contribution to journalArticle

Abstract

To investigate the role of epidermal growth factor (EGF) in human milk on protein digestion in intestinal cells, the effects of EGF on leucine aminopeptidase (LAP) activity and on LAP protein were examined in human colon adenocarcinoma (Caco-2) cells. The Caco-2 cells were grown in MEM supplemented with 20% fetal bovine serum, 50 U/ml penicillin and 50 μg/ml streptomycin. Upon 80% confluency, the cells were switched to serum-free MEM for 24 h. The cells were then incubated with EGF at 0, 5 (close to human milk concentration), 50 or 250 nM for 24 h. The total cellular protein content was significantly higher in the 5 nM EGF treatment group (1.76 ± 0.67 ng/cell) than others, although the cell numbers were similar among the groups. The LAP activity was slightly lower in the EGF treatment groups (78% - 86% of control). The LAP protein (MW 53 kDa) separated by 10% SDS-PAGE and detected by western blotting in the EGF treatment groups was also slightly lower (86% - 93% of control). In summary, EGF treatment increased protein content, but slightly decreased LAP activity and protein on the cell membranes of Caco-2 cells.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - 1997

Fingerprint

Leucyl Aminopeptidase
leucyl aminopeptidase
epidermal growth factor
Epidermal Growth Factor
colon
Colon
Caco-2 Cells
cells
Proteins
Human Milk
breast milk
proteins
protein content
Milk Proteins
Streptomycin
Cell membranes
Serum
fetal bovine serum
Penicillins
penicillins

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Effect of epidermal growth factor on leucine aminopeptidase in human colon (CACO-2) cells. / Chao, J. C J; Shieh, W. H S; Tavlor, A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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