Effect of chronic stress on expression and secretion of seminal vesicle proteins in adult rats

Sitthichai Iamsaard, Saranya Tongpan, Supataechasit Yannasithinon, Supatcharee Arun, Alexander T.H. Wu, Wannisa Sukhorum

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Chronic stress (CS) is known to affect men's health especially fertility by reducing semen quality. Although the effects of CS on testicular function and sperm parameters are documented, changes of substances and secreting proteins in the seminal vesicle (SV) have never been reported. This study aimed to demonstrate the alterations of contents and expressions of proteins in seminal vesicle fluid (SVF) under CS. Fourteen adult rats were divided into control and CS groups (n = 7/each). Control rats were not exposed to stressor, while the CS animals were immobilised by restraint cage (4 hr/day) and followed by forced swimming (15 min/day) for consecutive 60 days. Biochemical substances and malondialdehyde (MDA) level in SVF were examined. Expressions of heat-shock protein 70 (Hsp70), caspases (Casp) 3 and 9, and tyrosine-phosphorylated (TyrPho) proteins were investigated in seminal vesicle tissue (SVT) and SVF. It was found that CS caused reductions of seminal epithelial height and secreted substance levels. Significantly, MDA levels in SVF and expressions of Hsp70, Casp and TyrPho proteins were increased in of CS animals. It was concluded that CS affected seminal secretion. Low quality of CS seminal plasma may associate with increase of MDA and expressions of secreted proteins.

Original languageEnglish
Article numbere13800
JournalAndrologia
Volume53
Issue number1
DOIs
Publication statusPublished - Feb 2021

Keywords

  • caspase
  • chronic stress
  • heat-shock protein 70
  • seminal vesicle
  • tyrosine-phosphorylated proteins

ASJC Scopus subject areas

  • Endocrinology
  • Urology

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