Effect of chitosan on hepatic drug-metabolizing enzymes and oxidative stress in rats fed low- and high-fat diets

Hsien Tsung Yao, Chong Kuei Lii, Ruey Hwang Chou, Jia Hsuan Lin, Hui Ting Yang, Meng Tsan Chiang

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13 Citations (Scopus)

Abstract

Chitosan is sold worldwide as a lipid-lowering functional food and may be taken with certain medications. To investigate the effect of chitosan on drug-metabolizing enzymes and oxidative stress in the liver, male Wistar rats were fed a low- or high-fat diet with cellulose or chitosan for 4 weeks. A significant decrease in cytochrome P450 (CYP) 3A-catalyzed testosterone 6β-hydroxylation in liver microsomes was observed in rats fed the chitosan with low- and high-fat diets. The expression of CYP 3A1 and 3A2, however, was suppressed by chitosan in rats fed the low-fat diet only. Furthermore, rats fed the low-fat diet with chitosan had lower hepatic glutathione S-transferase (GST) activity and superoxide dismutase activity and higher total tissue and microsomal lipid hydroperoxides. Hepatic α-tocopherol was lower in rats fed the chitosan-containing diet. The results suggest that chitosan is likely to modulate CYP 3A activity and protein expression and GST activity partially in a dietary fat-dependent manner. This change may cause a decrease in the metabolism of drugs catalyzed by these enzymes in liver tissues. Moreover, decrease of α-tocopherol level and SOD activity by chitosan partly accounts for the increase of hepatic lipid peroxidation.

Original languageEnglish
Pages (from-to)5187-5193
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number8
DOIs
Publication statusPublished - Apr 28 2010
Externally publishedYes

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Keywords

  • Chitosan
  • CYP
  • Drug-metabolizing enzymes
  • Oxidative stress
  • Rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

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