Effect of chitosan on hepatic drug-metabolizing enzymes and oxidative stress in rats fed low- and high-fat diets

Hsien Tsung Yao, Chong Kuei Lii, Ruey Hwang Chou, Jia Hsuan Lin, Hui Ting Yang, Meng Tsan Chiang

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Chitosan is sold worldwide as a lipid-lowering functional food and may be taken with certain medications. To investigate the effect of chitosan on drug-metabolizing enzymes and oxidative stress in the liver, male Wistar rats were fed a low- or high-fat diet with cellulose or chitosan for 4 weeks. A significant decrease in cytochrome P450 (CYP) 3A-catalyzed testosterone 6β-hydroxylation in liver microsomes was observed in rats fed the chitosan with low- and high-fat diets. The expression of CYP 3A1 and 3A2, however, was suppressed by chitosan in rats fed the low-fat diet only. Furthermore, rats fed the low-fat diet with chitosan had lower hepatic glutathione S-transferase (GST) activity and superoxide dismutase activity and higher total tissue and microsomal lipid hydroperoxides. Hepatic α-tocopherol was lower in rats fed the chitosan-containing diet. The results suggest that chitosan is likely to modulate CYP 3A activity and protein expression and GST activity partially in a dietary fat-dependent manner. This change may cause a decrease in the metabolism of drugs catalyzed by these enzymes in liver tissues. Moreover, decrease of α-tocopherol level and SOD activity by chitosan partly accounts for the increase of hepatic lipid peroxidation.

Original languageEnglish
Pages (from-to)5187-5193
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume58
Issue number8
DOIs
Publication statusPublished - Apr 28 2010
Externally publishedYes

Fingerprint

Oxidative stress
Chitosan
High Fat Diet
high fat diet
Nutrition
chitosan
Rats
Oxidative Stress
oxidative stress
Fats
drugs
liver
Liver
rats
Enzymes
lipids
enzymes
Pharmaceutical Preparations
cytochrome P-450
Cytochrome P-450 CYP3A

Keywords

  • Chitosan
  • CYP
  • Drug-metabolizing enzymes
  • Oxidative stress
  • Rats

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)

Cite this

Effect of chitosan on hepatic drug-metabolizing enzymes and oxidative stress in rats fed low- and high-fat diets. / Yao, Hsien Tsung; Lii, Chong Kuei; Chou, Ruey Hwang; Lin, Jia Hsuan; Yang, Hui Ting; Chiang, Meng Tsan.

In: Journal of Agricultural and Food Chemistry, Vol. 58, No. 8, 28.04.2010, p. 5187-5193.

Research output: Contribution to journalArticle

Yao, Hsien Tsung ; Lii, Chong Kuei ; Chou, Ruey Hwang ; Lin, Jia Hsuan ; Yang, Hui Ting ; Chiang, Meng Tsan. / Effect of chitosan on hepatic drug-metabolizing enzymes and oxidative stress in rats fed low- and high-fat diets. In: Journal of Agricultural and Food Chemistry. 2010 ; Vol. 58, No. 8. pp. 5187-5193.
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