Effect of cell culture biomaterials for completely xeno-free generation of human induced pluripotent stem cells

Tzu Cheng Sung, Hsing Fen Li, Akon Higuchi, S. Suresh Kumar, Qing Dong Ling, Yu Wen Wu, Thierry Burnouf, Michiyo Nasu, Akihiro Umezawa, Kuei Fang Lee, Han Chow Wang, Yung Chang, Shih Tien Hsu

Research output: Contribution to journalArticle

Abstract

Human induced pluripotent stem cells (hiPSCs) were generated on several biomaterials from human amniotic fluid in completely xeno-free and feeder-free conditions via the transfection of pluripotent genes using a nonintegrating RNA Sendai virus vector. The effect of xeno-free culture medium on the efficiency of the establishment of human amniotic fluid stem cells from amniotic fluid was evaluated. Subsequently, the effect of cell culture biomaterials on the reprogramming efficiency was investigated during the reprogramming of human amniotic fluid stem cells into hiPSCs. Cells cultured in laminin-511, laminin-521, and Synthemax II-coated dishes and hydrogels having optimal elasticity that were engrafted with specific oligopeptides derived from vitronectin could be reprogrammed into hiPSCs with high efficiency. The reprogrammed cells expressed pluripotency proteins and had the capability to differentiate into cells derived from all three germ layers in vitro and in vivo. Human iPSCs could be generated successfully and at high efficiency (0.15–0.25%) in completely xeno-free conditions from the selection of optimal cell culture biomaterials.

Original languageEnglish
Article number119638
JournalBiomaterials
Volume230
DOIs
Publication statusPublished - Feb 2020

Fingerprint

Induced Pluripotent Stem Cells
Biocompatible Materials
Amniotic Fluid
Stem cells
Cell culture
Biomaterials
Cell Culture Techniques
Fluids
Stem Cells
Vitronectin
Sendai virus
Germ Layers
Oligopeptides
Hydrogels
RNA Viruses
Elasticity
Laminin
Transfection
Culture Media
Cultured Cells

Keywords

  • Extracellular matrix
  • Human induced pluripotent stem cell
  • Human platelet lysate
  • Hydrogel
  • Oligopeptide
  • Xeno-free culture

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Effect of cell culture biomaterials for completely xeno-free generation of human induced pluripotent stem cells. / Sung, Tzu Cheng; Li, Hsing Fen; Higuchi, Akon; Kumar, S. Suresh; Ling, Qing Dong; Wu, Yu Wen; Burnouf, Thierry; Nasu, Michiyo; Umezawa, Akihiro; Lee, Kuei Fang; Wang, Han Chow; Chang, Yung; Hsu, Shih Tien.

In: Biomaterials, Vol. 230, 119638, 02.2020.

Research output: Contribution to journalArticle

Sung, TC, Li, HF, Higuchi, A, Kumar, SS, Ling, QD, Wu, YW, Burnouf, T, Nasu, M, Umezawa, A, Lee, KF, Wang, HC, Chang, Y & Hsu, ST 2020, 'Effect of cell culture biomaterials for completely xeno-free generation of human induced pluripotent stem cells', Biomaterials, vol. 230, 119638. https://doi.org/10.1016/j.biomaterials.2019.119638
Sung, Tzu Cheng ; Li, Hsing Fen ; Higuchi, Akon ; Kumar, S. Suresh ; Ling, Qing Dong ; Wu, Yu Wen ; Burnouf, Thierry ; Nasu, Michiyo ; Umezawa, Akihiro ; Lee, Kuei Fang ; Wang, Han Chow ; Chang, Yung ; Hsu, Shih Tien. / Effect of cell culture biomaterials for completely xeno-free generation of human induced pluripotent stem cells. In: Biomaterials. 2020 ; Vol. 230.
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