Effect of catechin on the activity and gene expression of superoxide dismutase in cultured rat brain astrocytes

Paul Chan, Juei Tang Cheng, Jen Chen Tsai, Gi Shih Lien, Fu Chean Chen, Pai Feng Kao, Ju Chi Liu, Yi Jen Chen, Min Hsing Hsieh

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Stroke is one of the major causes of morbidity and mortality in recent. Oxygen free radicals produced during cerebral infarction increases the damage to neurons. Superoxide dismutase (SOD) is the endogenous antioxidant enzyme that can effectively scavenge superoxide radicals. Catechin is a hydrophilic antioxidant usually existed in tea, fruits and vegetables. In the cultured rat brain astrocytes (RBA), the activity of SOD (both Cu, Zn-SOD and Mn-SOD subtypes) was markedly increased by incubation with catechin at low concentration (0.1 μM) for 2 days (short-term) and 7 days (long-term). This stimulatory effect of catechin was not related to the incubating concentration. Similar changes were also observed in the gene expression of SOD in RBA. The increase in quantity of SOD-messenger RNA was remarkable and seemed to be more obvious than the other antioxidants such as vitamin E. This result shows that catechin is an effective antioxidant to increase the activity of SOD in RBA which would be beneficial to neurons subjected to oxygen free radical damage.

Original languageEnglish
Pages (from-to)281-284
Number of pages4
JournalNeuroscience Letters
Volume328
Issue number3
DOIs
Publication statusPublished - Aug 16 2002

Fingerprint

Catechin
Astrocytes
Superoxide Dismutase
Gene Expression
Brain
Antioxidants
Free Radicals
Reactive Oxygen Species
Neurons
Cerebral Infarction
Tea
Vitamin E
Superoxides
Vegetables
Fruit
Stroke
Morbidity
Messenger RNA
Mortality
Enzymes

Keywords

  • Antioxidant
  • Catechin
  • Cultured rat brain astrocytes
  • Gene expression
  • Superoxide dismutase

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Effect of catechin on the activity and gene expression of superoxide dismutase in cultured rat brain astrocytes. / Chan, Paul; Cheng, Juei Tang; Tsai, Jen Chen; Lien, Gi Shih; Chen, Fu Chean; Kao, Pai Feng; Liu, Ju Chi; Chen, Yi Jen; Hsieh, Min Hsing.

In: Neuroscience Letters, Vol. 328, No. 3, 16.08.2002, p. 281-284.

Research output: Contribution to journalArticle

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