Effect of calmodulin antagonists on the interferon system

induction and action of interferons

H. Y. Lin, P. J. Davis, F. B. Davis, K. C. Chadha, H. R. Thacore

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Synthesis of interferon (IFN) in response to Sendai virus and the development of the resulting antiviral state were studied in human (BG-9) and murine (L-929) fibroblast cell cultures in the presence of the calmodulin antagonists, trifluoperazine (TFP) and N-(6-aminohexyl)-5-chloro-1-naphthalenesulfonamide (W-7). Compared to control cultures, 16-fold and 8-fold more IFN was formed in human and murine cells, respectively, when 10 μM TFP was present in the medium for 24 h prior to IFN induction with Sendai virus. W-7 did not affect IFN production in human cells, but enhanced it in L-929 cells by 4- to 8-fold. TFP inhibited the antiviral state induced by homologous IFN in the two cell systems, and at 20 μM, there was a 3,000-fold increase in vesicular stomatitis virus (VSV) yield. It also reduced the maintenance of the antiviral state in human cells. In contrast, W-7 had no effect on the development of the antiviral state in either of the two cell systems. Thus, calcium-calmodulin dependent cellular processes are involved in both induction of IFN and its action. The several patterns response to TFP and W-7 may reflect different ligand-binding sites on calmodulin.

Original languageEnglish
Pages (from-to)39-46
Number of pages8
JournalJournal of Interferon Research
Volume10
Issue number1
Publication statusPublished - 1990
Externally publishedYes

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Calmodulin
Interferons
Trifluoperazine
Antiviral Agents
Sendai virus
Vesicular Stomatitis
Cell Culture Techniques
Fibroblasts
Binding Sites
Maintenance
W 7
Ligands
Viruses
Calcium

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Effect of calmodulin antagonists on the interferon system : induction and action of interferons. / Lin, H. Y.; Davis, P. J.; Davis, F. B.; Chadha, K. C.; Thacore, H. R.

In: Journal of Interferon Research, Vol. 10, No. 1, 1990, p. 39-46.

Research output: Contribution to journalArticle

Lin, H. Y. ; Davis, P. J. ; Davis, F. B. ; Chadha, K. C. ; Thacore, H. R. / Effect of calmodulin antagonists on the interferon system : induction and action of interferons. In: Journal of Interferon Research. 1990 ; Vol. 10, No. 1. pp. 39-46.
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