Abstract

Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.
Original languageEnglish
Pages (from-to)30-37
Number of pages8
JournalJournal of Dental Sciences
Volume3
Issue number1
Publication statusPublished - 2008

Fingerprint

Calcium Sulfate
Osteopontin
Osteosarcoma
Osteoblasts
Osteogenesis
Messenger RNA
Reverse Transcription
Western Blotting
Oral Medicine
Polymerase Chain Reaction
Proteins
Bone Regeneration
Dentists
Cultured Cells
Down-Regulation
Cell Proliferation

Keywords

  • calcium sulfate
  • osteopontin
  • osteoblasts

Cite this

Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells. / Chang, Yuan-Chieh; Wang, Duen-Jeng; Lee, Sheng-Yang; Chang, Wen-Chien; Yang, Wei-Chung Vivian.

In: Journal of Dental Sciences, Vol. 3, No. 1, 2008, p. 30-37.

Research output: Contribution to journalArticle

Chang, Yuan-Chieh ; Wang, Duen-Jeng ; Lee, Sheng-Yang ; Chang, Wen-Chien ; Yang, Wei-Chung Vivian. / Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells. In: Journal of Dental Sciences. 2008 ; Vol. 3, No. 1. pp. 30-37.
@article{ad8f40d9f11c41db9ef81d7f16cfb0b9,
title = "Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells",
abstract = "Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.",
keywords = "calcium sulfate, osteopontin, osteoblasts",
author = "Yuan-Chieh Chang and Duen-Jeng Wang and Sheng-Yang Lee and Wen-Chien Chang and Yang, {Wei-Chung Vivian}",
year = "2008",
language = "English",
volume = "3",
pages = "30--37",
journal = "Journal of Dental Sciences",
issn = "1991-7902",
publisher = "中華牙醫學會",
number = "1",

}

TY - JOUR

T1 - Effect of Calcium Sulfate on Osteopontin Expression in U-2 Osteosarcoma Cells

AU - Chang, Yuan-Chieh

AU - Wang, Duen-Jeng

AU - Lee, Sheng-Yang

AU - Chang, Wen-Chien

AU - Yang, Wei-Chung Vivian

PY - 2008

Y1 - 2008

N2 - Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.

AB - Calcium sulfate has been used for bone regeneration in oral medicine. Osteopontin, a marker molecule of osteoblasts, plays multiple roles in bone formation. However, the effect of calcium sulfate on osteopontin expression in osteoblasts remains unclear. In this study, U-2 osteosarcoma (U-20S) osteoblast-like cells were cultured in medium containing 0, 0.5, 1, or 10 μM calcium sulfate hemihydrate for 6 days. The messenger (m) RNA and protein expressions of osteopontin were respectively analyzed by reverse-transcription polymerase chain reaction (RT-PCR) and Western blotting. In the in vitro culture for 1-2 days, there was no effect on the proliferation of cells, suggesting that calcium sulfate at up to 10μM is biocompatible. RT-PCR results showed that a lower concentration of calcium sulfate (< 1μM) upregulated the osteopontin mRNA level of U-2OS cells. However, the protein expression of osteopontin was downregulated as the concentration of calcium sulfate increased in cultured U-2OS osteoblastic cells. Dissolved •Ca(superscript 2+) and SO(superscript 2- subscript 4) from calcium sulfate hemihydrates may alter the structure of secreted osteopontin, which then was not recognizable by the antibody in the Western blot analysis. In summary, calcium sulfate can regulate the mRNA and protein expressions of osteopontin. Controlling the degradation of calcium sulfate implants is important for regulating bone formation. This study provides additional information to help dentists understand the effects of calcium sulfate on bone formation and use calcium sulfate more efficiently in treating bone defects.

KW - calcium sulfate

KW - osteopontin

KW - osteoblasts

M3 - Article

VL - 3

SP - 30

EP - 37

JO - Journal of Dental Sciences

JF - Journal of Dental Sciences

SN - 1991-7902

IS - 1

ER -