Effect of butylidenephthalide on calcium mobilization in isolated rat aorta

Wun-Chang Ko, Chuan Y. Charng, Joen Rong Sheu, Shu-Huey Tzeng, Chi Ming Chen

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Butylidenephthalide (Bdph), an antispasmodic compound originally isolated from the rhizome of Ligusticum chuaxiong, has a selective anti-anginal effect without changing blood pressure. Experiments have been performed to determine the mechanism of this action. Synthetic Z-butylidenephthalide concentration-dependently relaxed phenylephrine (1 μM)- or KCl (60 mM)-induced precontractions of intact and denuded rat aorta rings. The relaxation induced by Bdph was endothelium-independent. Bdph (30-300 μM) concentration-dependently reduced cumulative phenylephrine- and KCl-induced contractions of intact rat aortic rings and non-competitively inhibited their log concentration-response curves. The pD2' values of Bdph for phenylephrine- and KCl-induced contraction were 3.66 ± 0.13 (n = 8) and 3.71 ± 0.07 (n = 8), respectively, which were not significantly different from each other. Bdph also concentration-dependently reduced cumulative Ca2+-induced contractions of intact rat aortic rings in high-KCl (60 mM) Ca2+-free physiological salt solution and non-competitively inhibited its log concentration-response curve. The pD2' value of Bdph for the Ca2+-induced contractions was 3.21 ± 0.01 (n = 7) which was significantly different from the pD2' value obtained from the cumulative KCl-induced contractions. These results suggest that Bdph inhibits calcium release from calcium stores more selectively than calcium influx from extracellular space via voltage-dependent calcium channels. The inhibition by Bdph of calcium release from KCl-sensitive calcium stores might be similar to its inhibition of calcium release from phenylephrine-sensitive calcium stores. However, because phenylephrine generates inositol-1,4,5-trisphosphate (IP3) whereas KCl does not, the inhibitory effect of Bdph might not be related to IP3 production.

Original languageEnglish
Pages (from-to)1365-1369
Number of pages5
JournalJournal of Pharmacy and Pharmacology
Volume50
Issue number12
Publication statusPublished - Dec 1998

Fingerprint

Aorta
Calcium
Phenylephrine
Ligusticum
butylidenephthalide
Parasympatholytics
Rhizome
Inositol 1,4,5-Trisphosphate
Extracellular Space
Calcium Channels
Endothelium
Salts
Blood Pressure

ASJC Scopus subject areas

  • Pharmaceutical Science
  • Pharmacology

Cite this

Ko, W-C., Charng, C. Y., Sheu, J. R., Tzeng, S-H., & Chen, C. M. (1998). Effect of butylidenephthalide on calcium mobilization in isolated rat aorta. Journal of Pharmacy and Pharmacology, 50(12), 1365-1369.

Effect of butylidenephthalide on calcium mobilization in isolated rat aorta. / Ko, Wun-Chang; Charng, Chuan Y.; Sheu, Joen Rong; Tzeng, Shu-Huey; Chen, Chi Ming.

In: Journal of Pharmacy and Pharmacology, Vol. 50, No. 12, 12.1998, p. 1365-1369.

Research output: Contribution to journalArticle

Ko, W-C, Charng, CY, Sheu, JR, Tzeng, S-H & Chen, CM 1998, 'Effect of butylidenephthalide on calcium mobilization in isolated rat aorta', Journal of Pharmacy and Pharmacology, vol. 50, no. 12, pp. 1365-1369.
Ko, Wun-Chang ; Charng, Chuan Y. ; Sheu, Joen Rong ; Tzeng, Shu-Huey ; Chen, Chi Ming. / Effect of butylidenephthalide on calcium mobilization in isolated rat aorta. In: Journal of Pharmacy and Pharmacology. 1998 ; Vol. 50, No. 12. pp. 1365-1369.
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