Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro.

S. U. Chen, H. N. Ho, H. F. Chen, K. H. Chao, M. Y. Wu, S. C. Huang, T. Y. Lee, Y. S. Yang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The zonae pellucida of mouse embryos were dissected to examine the effect of partial zona dissection (PZD) at the four-cell stage on embryonic development and the hatching process in vitro. Micropipettes were used to create a gap of one-sixth the zona circumference, to avoid damage to the blastomere. A total of 484 embryos at the four-cell stage (defined as day 2 embryos) were randomly divided into two groups, the PZD group (n = 238) and the control group (n = 246). Embryos of both groups were cultured in vitro under identical conditions and examined on the following day. The hatching rates were as follows: day 3, 11/238 (4.6%) in the PZD group and 0/246 (0%) in the control group; day 4, 105/238 (44.1%) in the PZD group and 54/246 (22.0%) in the control group; day 5, 71/238 (29.8%) in the PZD group and 36/245 (14.5%) in the control group. On day 4 the rates of complete hatching were 7/238 (2.9%) in the PZD group and 2/246 (0.8%) in the control group. On day 6 they were 88/238 (37.0%) in the PZD group and 66/246 (26.8%) in the control group. A significantly higher percentage of PZD embryos than controls initiated and completed hatching in similar culture conditions. The present study indicates that the creation of an opening in the zona pellucida is not harmful to embryonic development and may facilitate hatching. Whether the enhanced hatching ability of embryos in vitro caused by PZD can improve the implantation rate requires further study.

Original languageEnglish
Pages (from-to)463-468
Number of pages6
JournalJournal of the Formosan Medical Association = Taiwan yi zhi
Volume94
Issue number8
Publication statusPublished - Jan 1 1995
Externally publishedYes

Fingerprint

Micromanipulation
Zona Pellucida
Inbred ICR Mouse
Embryo Transfer
Herpes Zoster
Dissection
Embryonic Structures
Pregnancy
Control Groups
Embryonic Development
In Vitro Techniques
Blastomeres

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro. / Chen, S. U.; Ho, H. N.; Chen, H. F.; Chao, K. H.; Wu, M. Y.; Huang, S. C.; Lee, T. Y.; Yang, Y. S.

In: Journal of the Formosan Medical Association = Taiwan yi zhi, Vol. 94, No. 8, 01.01.1995, p. 463-468.

Research output: Contribution to journalArticle

Chen, SU, Ho, HN, Chen, HF, Chao, KH, Wu, MY, Huang, SC, Lee, TY & Yang, YS 1995, 'Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro.', Journal of the Formosan Medical Association = Taiwan yi zhi, vol. 94, no. 8, pp. 463-468.
Chen, S. U. ; Ho, H. N. ; Chen, H. F. ; Chao, K. H. ; Wu, M. Y. ; Huang, S. C. ; Lee, T. Y. ; Yang, Y. S. / Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro. In: Journal of the Formosan Medical Association = Taiwan yi zhi. 1995 ; Vol. 94, No. 8. pp. 463-468.
@article{fcd1cf600b694f81a0767826458999aa,
title = "Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro.",
abstract = "The zonae pellucida of mouse embryos were dissected to examine the effect of partial zona dissection (PZD) at the four-cell stage on embryonic development and the hatching process in vitro. Micropipettes were used to create a gap of one-sixth the zona circumference, to avoid damage to the blastomere. A total of 484 embryos at the four-cell stage (defined as day 2 embryos) were randomly divided into two groups, the PZD group (n = 238) and the control group (n = 246). Embryos of both groups were cultured in vitro under identical conditions and examined on the following day. The hatching rates were as follows: day 3, 11/238 (4.6{\%}) in the PZD group and 0/246 (0{\%}) in the control group; day 4, 105/238 (44.1{\%}) in the PZD group and 54/246 (22.0{\%}) in the control group; day 5, 71/238 (29.8{\%}) in the PZD group and 36/245 (14.5{\%}) in the control group. On day 4 the rates of complete hatching were 7/238 (2.9{\%}) in the PZD group and 2/246 (0.8{\%}) in the control group. On day 6 they were 88/238 (37.0{\%}) in the PZD group and 66/246 (26.8{\%}) in the control group. A significantly higher percentage of PZD embryos than controls initiated and completed hatching in similar culture conditions. The present study indicates that the creation of an opening in the zona pellucida is not harmful to embryonic development and may facilitate hatching. Whether the enhanced hatching ability of embryos in vitro caused by PZD can improve the implantation rate requires further study.",
author = "Chen, {S. U.} and Ho, {H. N.} and Chen, {H. F.} and Chao, {K. H.} and Wu, {M. Y.} and Huang, {S. C.} and Lee, {T. Y.} and Yang, {Y. S.}",
year = "1995",
month = "1",
day = "1",
language = "English",
volume = "94",
pages = "463--468",
journal = "Journal of the Formosan Medical Association",
issn = "0929-6646",
publisher = "Elsevier Science Publishers B.V.",
number = "8",

}

TY - JOUR

T1 - Effect of assisted hatching by partial zona pellucida dissection on mouse embryos in vitro.

AU - Chen, S. U.

AU - Ho, H. N.

AU - Chen, H. F.

AU - Chao, K. H.

AU - Wu, M. Y.

AU - Huang, S. C.

AU - Lee, T. Y.

AU - Yang, Y. S.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - The zonae pellucida of mouse embryos were dissected to examine the effect of partial zona dissection (PZD) at the four-cell stage on embryonic development and the hatching process in vitro. Micropipettes were used to create a gap of one-sixth the zona circumference, to avoid damage to the blastomere. A total of 484 embryos at the four-cell stage (defined as day 2 embryos) were randomly divided into two groups, the PZD group (n = 238) and the control group (n = 246). Embryos of both groups were cultured in vitro under identical conditions and examined on the following day. The hatching rates were as follows: day 3, 11/238 (4.6%) in the PZD group and 0/246 (0%) in the control group; day 4, 105/238 (44.1%) in the PZD group and 54/246 (22.0%) in the control group; day 5, 71/238 (29.8%) in the PZD group and 36/245 (14.5%) in the control group. On day 4 the rates of complete hatching were 7/238 (2.9%) in the PZD group and 2/246 (0.8%) in the control group. On day 6 they were 88/238 (37.0%) in the PZD group and 66/246 (26.8%) in the control group. A significantly higher percentage of PZD embryos than controls initiated and completed hatching in similar culture conditions. The present study indicates that the creation of an opening in the zona pellucida is not harmful to embryonic development and may facilitate hatching. Whether the enhanced hatching ability of embryos in vitro caused by PZD can improve the implantation rate requires further study.

AB - The zonae pellucida of mouse embryos were dissected to examine the effect of partial zona dissection (PZD) at the four-cell stage on embryonic development and the hatching process in vitro. Micropipettes were used to create a gap of one-sixth the zona circumference, to avoid damage to the blastomere. A total of 484 embryos at the four-cell stage (defined as day 2 embryos) were randomly divided into two groups, the PZD group (n = 238) and the control group (n = 246). Embryos of both groups were cultured in vitro under identical conditions and examined on the following day. The hatching rates were as follows: day 3, 11/238 (4.6%) in the PZD group and 0/246 (0%) in the control group; day 4, 105/238 (44.1%) in the PZD group and 54/246 (22.0%) in the control group; day 5, 71/238 (29.8%) in the PZD group and 36/245 (14.5%) in the control group. On day 4 the rates of complete hatching were 7/238 (2.9%) in the PZD group and 2/246 (0.8%) in the control group. On day 6 they were 88/238 (37.0%) in the PZD group and 66/246 (26.8%) in the control group. A significantly higher percentage of PZD embryos than controls initiated and completed hatching in similar culture conditions. The present study indicates that the creation of an opening in the zona pellucida is not harmful to embryonic development and may facilitate hatching. Whether the enhanced hatching ability of embryos in vitro caused by PZD can improve the implantation rate requires further study.

UR - http://www.scopus.com/inward/record.url?scp=0029351722&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029351722&partnerID=8YFLogxK

M3 - Article

VL - 94

SP - 463

EP - 468

JO - Journal of the Formosan Medical Association

JF - Journal of the Formosan Medical Association

SN - 0929-6646

IS - 8

ER -