Effect of Antrodia camphorata on inflammatory arterial thrombosis-mediated platelet activation: The pivotal role of protein kinase C

Wan-Jung Lu, Shih Chang Lin, Chang Chou Lan, Tzu Yin Lee, Chih Hsuan Hsia, Yung Kai Huang, Hsiu Chuan Lee, Joen Rong Sheu

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Antrodia camphorata is a rare Taiwanese medicinal mushroom. Antrodia camphorata extract has been reported to exhibit antioxidant, anti-inflammation, antimetastasis, and anticancer activities and plays a role in liver fibrosis, vasorelaxation, and immunomodulation. Critical vascular inflammation leads to vascular dysfunction and cardiovascular diseases, including abdominal aortic aneurysms, hypertension, and atherosclerosis. Platelet activation plays a crucial role in intravascular thrombosis, which is involved in a wide variety of cardiovascular diseases. However, the effect of Antrodia camphorata on platelet activation remains unclear. We examined the effects of Antrodia camphorata on platelet activation. In the present study, Antrodia camphorata treatment (56-224 μg/mL) inhibited platelet aggregation induced by collagen, but not U46619, an analogue of thromboxane A2, thrombin, and arachidonic acid. Antrodia camphorata inhibited collagen-induced calcium (Ca 2 +) mobilization and phosphorylation of protein kinase C (PKC) and Akt. In addition, Antrodia camphorata significantly reduced the aggregation and phosphorylation of PKC in phorbol-12, 13-dibutyrate (PDBu) activated platelets. In conclusion, Antrodia camphorata may inhibit platelet activation by inhibiting of Ca 2 + and PKC cascade and the Akt pathway. Our study suggests that Antrodia camphorata may be a potential therapeutic agent for preventing or treating thromboembolic disorders.

Original languageEnglish
Article number745802
JournalScientific World Journal
Volume2014
DOIs
Publication statusPublished - 2014

Fingerprint

Antrodia
Platelet Activation
Platelets
Protein Kinase C
Thrombosis
collagen
Chemical activation
cardiovascular disease
protein
hypertension
Phosphorylation
mushroom
antioxidant
mobilization
Collagen
Agglomeration
calcium
Phorbol 12,13-Dibutyrate
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid
Thromboxane A2

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Effect of Antrodia camphorata on inflammatory arterial thrombosis-mediated platelet activation : The pivotal role of protein kinase C. / Lu, Wan-Jung; Lin, Shih Chang; Lan, Chang Chou; Lee, Tzu Yin; Hsia, Chih Hsuan; Huang, Yung Kai; Lee, Hsiu Chuan; Sheu, Joen Rong.

In: Scientific World Journal, Vol. 2014, 745802, 2014.

Research output: Contribution to journalArticle

Lu, Wan-Jung ; Lin, Shih Chang ; Lan, Chang Chou ; Lee, Tzu Yin ; Hsia, Chih Hsuan ; Huang, Yung Kai ; Lee, Hsiu Chuan ; Sheu, Joen Rong. / Effect of Antrodia camphorata on inflammatory arterial thrombosis-mediated platelet activation : The pivotal role of protein kinase C. In: Scientific World Journal. 2014 ; Vol. 2014.
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