Effect of antenatal dexamethasone on the expression of endothelial nitric oxide synthase in the lungs of postnatal pups

Chyi Her Lin, Mei Ling Tsai, Shiao Jiun Chou, Tsu Fu Yeh

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Activities of endothelial nitric oxide synthase (eNOS) are developmentally regulated and its presence at birth may play a role in the transition of cardiopulmonary circulation. Antenatal dexamethasone (Dex) therapy accelerates fetal lung maturation. We speculate that Dex therapy may enhance pulmonary eNOS protein expression in the newborn. This article examines whether antenatal Dex therapy affected the expression of eNOS in the lungs of rat pups in the postnatal period. Time-dated pregnant Wistar rats were subjected to 2 doses of Dex (0.8 mg/kg, intramuscularly, daily) or equivalent volume of normal saline at the 18th and 19th gestational day and delivered naturally. The newborn pups were randomly assigned to 4 groups by age: days 1, 3, 5, and 7. After homogenization, abundance of eNOS protein in lungs was determined by Western blot analysis. There were 7 dams in each group. Mean body weights of the pups in the Dex group were lighter than those in the control at birth and remained stunted up to day 7 (5.68 ± 0.47 g v 6.34 ± 0.47 g, P <.01). However, there were no differences in wet lung weights and lung/body weight ratios between both groups in the study period. Abundance of eNOS protein expression decreased in both the control and Dex groups (P <.01). Pups that received antenatal Dex had 39% more in abundance of eNOS protein expression in lungs when compared to the control on day 1 (P < .05) but there were no differences between both groups from day 3 to 7. We conclude that antenatal Dex therapy enhances the abundance of eNOS protein expression in the lung at birth and could be a factor in improving respiratory functions in infants who received antenatal steroid therapy.

Original languageEnglish
Pages (from-to)94-99
Number of pages6
JournalSeminars in Perinatology
Volume25
Issue number2
DOIs
Publication statusPublished - Jan 1 2001
Externally publishedYes

Fingerprint

Nitric Oxide Synthase Type III
Dexamethasone
Lung
Proteins
Fetal Therapies
Body Weight
Parturition
Newborn Infant
Pulmonary Edema
Therapeutics
Contraception
Wistar Rats
Age Groups
Western Blotting
Steroids
Weights and Measures
Control Groups

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Obstetrics and Gynaecology

Cite this

Effect of antenatal dexamethasone on the expression of endothelial nitric oxide synthase in the lungs of postnatal pups. / Lin, Chyi Her; Tsai, Mei Ling; Chou, Shiao Jiun; Yeh, Tsu Fu.

In: Seminars in Perinatology, Vol. 25, No. 2, 01.01.2001, p. 94-99.

Research output: Contribution to journalArticle

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