Effect of 5-Aminolevulinic Acid-Mediated Photodynamic Therapy on MCF-7 and MCF-7/ADR Cells

Tsuimin Tsai, Ruey Long Hong, Jui Chang Tsai, Pei J. Lou, I. Fang Ling, Chin T. Chen

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Background and Objectives: Photodynamic therapy (PDT) has been proposed as an alternative approach in overcoming multidrug resistance (MDR) phenotype. To verify whether 5-aminolevulinic acid (ALA)-mediated PDT is effective in MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, and phototoxicity in human breast cancer cells MCF-7 and derived MDR subline, MCF-7/ADR. Study Design/Materials and Methods: The fluorescence kinetics of ALA-induced PpIX was evaluated by spectrofluorometer. The phototoxicity of MCF-7 and MCF-7/ADR cells was determined by tetrazolium (MTT) assays and clonogenic assay. Furthermore, Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after ALA-PDT. Results: MCF-7/ADR accumulated a lower level of PpIX as compared to parental MCF-7 cells. Significant phototoxicity was observed in MCF-7 and increased in a fluence-dependent manner with LD50 around 8 J/cm 2. Compared to its parental counterpart, MCF-7/ADR cells were less sensitive to ALA photodynamic treatment and PDT-induced cytotoxicity did not increase in a dose responsive manner as the concentration of ALA increased or the fluence of light increased. ALA-PDT was less effective for MCF-7/ADR cells than MCF-7 cells even under the condition when these two cell lines contained the similar amounts of PpIX. Conclusions: These results indicate that, except for the MDR related characteristics, MCF-7/ADR cells might possess intrinsic mechanisms that render them less sensitive to ALA-PDT induced phototoxicity.

Original languageEnglish
Pages (from-to)62-72
Number of pages11
JournalLasers in Surgery and Medicine
Volume34
Issue number1
DOIs
Publication statusPublished - 2004

Fingerprint

Aminolevulinic Acid
MCF-7 Cells
Photochemotherapy
Phototoxic Dermatitis
Multiple Drug Resistance
Propidium
Annexin A5
Lethal Dose 50
Cell Death
Fluorescence
Breast Neoplasms
Phenotype
Light
Cell Line
protoporphyrin IX

Keywords

  • Apoptosis
  • Multidrug resistance
  • Photodynamic therapy
  • Protoporphyrin IX

ASJC Scopus subject areas

  • Surgery

Cite this

Effect of 5-Aminolevulinic Acid-Mediated Photodynamic Therapy on MCF-7 and MCF-7/ADR Cells. / Tsai, Tsuimin; Hong, Ruey Long; Tsai, Jui Chang; Lou, Pei J.; Ling, I. Fang; Chen, Chin T.

In: Lasers in Surgery and Medicine, Vol. 34, No. 1, 2004, p. 62-72.

Research output: Contribution to journalArticle

Tsai, Tsuimin ; Hong, Ruey Long ; Tsai, Jui Chang ; Lou, Pei J. ; Ling, I. Fang ; Chen, Chin T. / Effect of 5-Aminolevulinic Acid-Mediated Photodynamic Therapy on MCF-7 and MCF-7/ADR Cells. In: Lasers in Surgery and Medicine. 2004 ; Vol. 34, No. 1. pp. 62-72.
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AU - Lou, Pei J.

AU - Ling, I. Fang

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AB - Background and Objectives: Photodynamic therapy (PDT) has been proposed as an alternative approach in overcoming multidrug resistance (MDR) phenotype. To verify whether 5-aminolevulinic acid (ALA)-mediated PDT is effective in MDR cells, we studied the protoporphyrin IX (PpIX) content, intracellular localization, and phototoxicity in human breast cancer cells MCF-7 and derived MDR subline, MCF-7/ADR. Study Design/Materials and Methods: The fluorescence kinetics of ALA-induced PpIX was evaluated by spectrofluorometer. The phototoxicity of MCF-7 and MCF-7/ADR cells was determined by tetrazolium (MTT) assays and clonogenic assay. Furthermore, Annexin V and propidium iodide (PI) binding assays were performed to analyze the characteristics of cell death after ALA-PDT. Results: MCF-7/ADR accumulated a lower level of PpIX as compared to parental MCF-7 cells. Significant phototoxicity was observed in MCF-7 and increased in a fluence-dependent manner with LD50 around 8 J/cm 2. Compared to its parental counterpart, MCF-7/ADR cells were less sensitive to ALA photodynamic treatment and PDT-induced cytotoxicity did not increase in a dose responsive manner as the concentration of ALA increased or the fluence of light increased. ALA-PDT was less effective for MCF-7/ADR cells than MCF-7 cells even under the condition when these two cell lines contained the similar amounts of PpIX. Conclusions: These results indicate that, except for the MDR related characteristics, MCF-7/ADR cells might possess intrinsic mechanisms that render them less sensitive to ALA-PDT induced phototoxicity.

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