Editing site analysis in a gymnosperm mitochondrial genome reveals similarities with angiosperm mitochondrial genomes

Michael Lee Salmans, Shu Miaw Chaw, Ching Ping Lin, Arthur Chun Chieh Shih, Yu Wei Wu, R. Michael Mulligan

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sequence analysis of organelle genomes and comprehensive analysis of C-to-U editing sites from flowering and non-flowering plants have provided extensive sequence information from diverse taxa. This study includes the first comprehensive analysis of RNA editing sites from a gymnosperm mitochondrial genome, and utilizes informatics analyses to determine conserved features in the RNA sequence context around editing sites. We have identified 565 editing sites in 21 full-length and 4 partial cDNAs of the 39 protein-coding genes identified from the mitochondrial genome of Cycas taitungensis. The information profiles and RNA sequence context of C-to-U editing sites in the Cycas genome exhibit similarity in the immediate flanking nucleotides. Relative entropy analyses indicate that similar regions in the 5' flanking 20 nucleotides have information content compared to angiosperm mitochondrial genomes. These results suggest that evolutionary constraints exist on the nucleotide sequences immediately adjacent to C-to-U editing sites, and similar regions are utilized in editing site recognition.

Original languageEnglish
Pages (from-to)439-446
Number of pages8
JournalCurrent Genetics
Volume56
Issue number5
DOIs
Publication statusPublished - Oct 2010
Externally publishedYes

Fingerprint

Gymnosperms
Angiosperms
Mitochondrial Genome
Cycas
Nucleotides
Genome
RNA Editing
Informatics
5' Flanking Region
Entropy
Organelles
Sequence Analysis
Complementary DNA
Proteins

Keywords

  • Organelle evolution
  • Relative entropy
  • RNA editing

ASJC Scopus subject areas

  • Genetics

Cite this

Editing site analysis in a gymnosperm mitochondrial genome reveals similarities with angiosperm mitochondrial genomes. / Salmans, Michael Lee; Chaw, Shu Miaw; Lin, Ching Ping; Shih, Arthur Chun Chieh; Wu, Yu Wei; Mulligan, R. Michael.

In: Current Genetics, Vol. 56, No. 5, 10.2010, p. 439-446.

Research output: Contribution to journalArticle

Salmans, Michael Lee ; Chaw, Shu Miaw ; Lin, Ching Ping ; Shih, Arthur Chun Chieh ; Wu, Yu Wei ; Mulligan, R. Michael. / Editing site analysis in a gymnosperm mitochondrial genome reveals similarities with angiosperm mitochondrial genomes. In: Current Genetics. 2010 ; Vol. 56, No. 5. pp. 439-446.
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