Edema-inducing activity of a lethal protein with phospholipase A1 activity isolated from the black-bellied hornet (Vespa basalis) venom

C. L. Ho, L. L. Hwang, C. T. Chen

Research output: Contribution to journalArticle

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Abstract

C. L. Ho, L. L. Hwang and C. T. Chen. Edema-inducing activity of a lethal protein with phospholipase A1 activity isolated from the black-bellied hornet (Vespa basalis) venom. Toxicon 31, 605-613, 1993.-The lethal protein of the hornet (Vespa basalis) venom is a phospholipase A1 toxin (mol. wt ∼ 32,000) with a potent hemolytic activity. Subplantar injection of the toxin caused a dose-dependent swelling in the rat hind paw. Its potency was higher than those of phospholipases A2 and cardiotoxin from cobra venoms. Hind-paw edema induced by the toxin was inhibited by antiserotonin drugs (cyproheptadine and methysergide), indomethacin and betamethasone. Antihistamine (chlorpheniramine) showed a relatively weak inhibition. Intradermal injection of the toxin into back skin of the rat also induced local edema which was inhibited by chlorpheniramine and methysergide. Rats pretreated with multiple doses of compound 48/80 showed a moderate decrease in the histamine and serotonin content of rat skin, and a slight decrease in paw edema induced by the toxin, while a single dose of reserpine markedly diminished the toxin-induced edema in association with depletion of serotonin in rat skin. The edema-inhibitory action of amine-depleting agents appeared to correlate with their potencies to deplete serotonin in the skin. It is suggested that serotonin, prostaglandin E2, and to a lesser extent of histamine are involved in producing the local effect of the toxin. However, serotonin released by the toxin appears to be the major factor mediating the toxin-induced edema in the rat.

Original languageEnglish
Pages (from-to)605-613
Number of pages9
JournalToxicon
Volume31
Issue number5
DOIs
Publication statusPublished - 1993
Externally publishedYes

Fingerprint

Phospholipases A1
Wasp Venoms
Wasps
Venoms
Rats
Edema
Serotonin
Skin
Chlorpheniramine
Methysergide
Proteins
Histamine
Cyproheptadine
Cobra Venoms
p-Methoxy-N-methylphenethylamine
Cardiotoxins
Betamethasone
Phospholipases A2
Histamine Antagonists
Reserpine

ASJC Scopus subject areas

  • Toxicology

Cite this

Edema-inducing activity of a lethal protein with phospholipase A1 activity isolated from the black-bellied hornet (Vespa basalis) venom. / Ho, C. L.; Hwang, L. L.; Chen, C. T.

In: Toxicon, Vol. 31, No. 5, 1993, p. 605-613.

Research output: Contribution to journalArticle

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