Echovirus 1 interaction with the human very late antigen-2 (integrin α2β1) I domain: Identification of two independent virus contact sites distinct from the metal ion-dependent adhesion site

Sandra L. King, Tetsuji Kamata, Jennifer A. Cunningham, Jonas Emsley, Robert C. Liddington, Yoshikazu Takada, Jeffrey M. Bergelson

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The human integrin very late antigen (VLA)-2 (CD49b/CD29) mediates interactions with collagen and is the receptor for echovirus 1. Binding sites for both collagen and echovirus 1 have been mapped to the I domain within the α2 subunit of the VLA-2 α2β1 heterodimer. Although marine VLA-2 interacts with collagen, it does not bind virus. We have used isolated human-murine chimeric I domains expressed as glutathione S-transferase fusion proteins in Escherichia coli to identify two groups of amino acids, 199-201 and 212-216, independently involved in virus attachment. These residues are distinct from the metal ion-dependent adhesion site previously demonstrated to be essential for VLA-2 interactions with collagen. Mutations in three metal ion-dependent adhesion site residues that abolish adhesion to collagen had no effect on virus binding. These results confirm that different sites within the I domain are responsible for VLA-2 interaction with extracellular matrix proteins and with vital ligands.

Original languageEnglish
Pages (from-to)28518-28522
Number of pages5
JournalJournal of Biological Chemistry
Volume272
Issue number45
DOIs
Publication statusPublished - Nov 7 1997
Externally publishedYes

Fingerprint

Integrin alpha2beta1
Human Enterovirus B
Viruses
Integrins
Metal ions
Adhesion
Metals
Ions
Collagen
Virus Attachment
Collagen Receptors
Extracellular Matrix Proteins
Escherichia coli Proteins
Glutathione Transferase
Escherichia coli
Fusion reactions
Binding Sites
Ligands
Amino Acids
Mutation

ASJC Scopus subject areas

  • Biochemistry

Cite this

Echovirus 1 interaction with the human very late antigen-2 (integrin α2β1) I domain : Identification of two independent virus contact sites distinct from the metal ion-dependent adhesion site. / King, Sandra L.; Kamata, Tetsuji; Cunningham, Jennifer A.; Emsley, Jonas; Liddington, Robert C.; Takada, Yoshikazu; Bergelson, Jeffrey M.

In: Journal of Biological Chemistry, Vol. 272, No. 45, 07.11.1997, p. 28518-28522.

Research output: Contribution to journalArticle

King, Sandra L. ; Kamata, Tetsuji ; Cunningham, Jennifer A. ; Emsley, Jonas ; Liddington, Robert C. ; Takada, Yoshikazu ; Bergelson, Jeffrey M. / Echovirus 1 interaction with the human very late antigen-2 (integrin α2β1) I domain : Identification of two independent virus contact sites distinct from the metal ion-dependent adhesion site. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 45. pp. 28518-28522.
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AU - Kamata, Tetsuji

AU - Cunningham, Jennifer A.

AU - Emsley, Jonas

AU - Liddington, Robert C.

AU - Takada, Yoshikazu

AU - Bergelson, Jeffrey M.

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