EBV-associated but HHV8-unrelated double-hit effusion-based lymphoma

Bo Jung Chen, David Yen Ting Chen, Chun Chi Kuo, Shih Sung Chuang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Effusion-based lymphoma is a rare and unique type of large B-cell lymphoma presenting in effusion without a mass lesion. It shares many clinicopathological features with primary effusion lymphoma (PEL), but is distinct from PEL by the absence of HHV8 association. Double hit lymphoma (DHL) is an aggressive B-cell lymphoma, defined by concurrent rearrangement of MYC and BCL2 or BCL6. DHL often presents as lymphadenopathy or an extranodal mass, but rarely occurs in effusion. Here we report a 61-year-old male with alcoholic cirrhosis presenting as massive ascites and left pleural effusion. He has no HIV, HBV or HCV infection and no mass lesion by CT scans. Cytology of both pleural effusion and ascites show large lymphoma cells with plasmablastic morphology characterized by pleomorphic and eccentric nuclei, prominent nucleoli and frequent mitoses. Immunohistochemical study with cell block shows that the lymphoma cells express plasma cell-related markers (CD138, MUM-1 and EMA), but not CD3, CD30, CD45, B-cell markers (CD19, CD20, CD79a, and PAX5), HHV8, ALK or cytokeratin. EBER is positive in most lymphoma cells. Fluorescence in situ hybridization reveals rearrangement at the IGH, BCL2, and MYC loci, but not at BCL6. It is diagnosed as an EBV-associated but HHV8-unrelated double hit effusion-based lymphoma with plasmablastic features. The patient passed away soon after diagnosis without chemotherapy. This is the first reported case of double-hit effusion-based lymphoma with MYC and BCL2 rearrangement. This case illustrates the importance of integrating clinical, cytological, immunophenotypical, and molecular findings to reach a correct diagnosis. Diagn. Cytopathol. 2017;45:257–261.

Original languageEnglish
Pages (from-to)257-261
Number of pages5
JournalDiagnostic Cytopathology
Volume45
Issue number3
DOIs
Publication statusPublished - Mar 1 2017

Fingerprint

Human Herpesvirus 4
Lymphoma
Primary Effusion Lymphoma
B-Cell Lymphoma
Pleural Effusion
Ascites
Alcoholic Liver Cirrhosis
Keratins
Plasma Cells
Fluorescence In Situ Hybridization
Mitosis
Cell Biology
B-Lymphocytes
HIV
Drug Therapy
Infection

Keywords

  • double hit lymphoma
  • effusion-based lymphoma
  • Epstein-Barr virus
  • human herpesvirus-8/Kaposi's-sarcoma associated herpes virus
  • primary effusion lymphoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology

Cite this

EBV-associated but HHV8-unrelated double-hit effusion-based lymphoma. / Chen, Bo Jung; Chen, David Yen Ting; Kuo, Chun Chi; Chuang, Shih Sung.

In: Diagnostic Cytopathology, Vol. 45, No. 3, 01.03.2017, p. 257-261.

Research output: Contribution to journalArticle

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