Early viral entry assays for the identification and evaluation of antiviral compounds

Chen Jei Tai, Chia Lin Li, Cheng Jeng Tai, Chien Kai Wang, Liang Tzung Lin

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cell-based systems are useful for discovering antiviral agents. Dissecting the viral life cycle, particularly the early entry stages, allows a mechanistic approach to identify and evaluate antiviral agents that target specific steps of the viral entry. In this report, the methods of examining viral inactivation, viral attachment, and viral entry/fusion as antiviral assays for such purposes are described, using hepatitis C virus as a model. These assays should be useful for discovering novel antagonists/inhibitors to early viral entry and help expand the scope of candidate antiviral agents for further drug development.

Original languageEnglish
Article numbere53124
JournalJournal of Visualized Experiments
Volume2015
Issue number104
DOIs
Publication statusPublished - Oct 29 2015

Keywords

  • Antiviral activity
  • Drug development
  • Early viral entry
  • Immunology
  • Issue 104
  • Viral attachment/binding
  • Viral entry/fusion
  • Viral inactivation

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Chemical Engineering(all)
  • Immunology and Microbiology(all)
  • Neuroscience(all)

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