Early intraarticular injection of hyaluronic acid attenuates osteoarthritis progression in anterior cruciate ligament-transected rats

Wei Yuan Tsai, Jia Lin Wu, Chih Chung Liu, Chen Hwan Cherng, Ru Yin Tsai, Yen Hsuan Jean, Chih Shung Wong

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Subject: Hyaluronic acid (HA) is widely used to relieve the symptoms of osteoarthritis (OA). An association of reduction of glutamate content with the synovial fluid of OA rats was reported previously. Design: Anterior cruciate ligament transaction (ACLT) was performed on one knee in male Wistar rats, the other knee was assigned to sham control and HA or saline was injected intraarticularly into the ACLT knee from week 3 to week 7. Knee dialysate was collected for amino acid measurement at week 20. Morphology and histopathology of the femoral medial condyles and synovium were examined and evaluated using Mankin and synovitis scores. Results: HA injection provided better cartilage (3.38 ± 0.03 vs. 5.45 ± 0.0.02) and synovial condition (3 ± 0.02 vs. 6.03 ± 0.02) than saline controls. Moreover, HA injection reduced the concentration of glutamates in knee dialysates compared to saline controls (1.11 ± 0.14-folds and 2.21 ± 0.19-folds of the sham-operated knee, respectively). Cystine/glutamate antiporter system expression was significantly downregulated in the saline group, but not in the HA group (0.32 ± 0.08-folds and 0.71 ± 0.10-folds of the sham-operated knee, respectively). Conclusion: Early intraarticular injection of HA attenuates the progression of cartilage destruction in the ACLT knee, and the downregulation of the cystine/glutamate antiporter system was accompanied by the progression of OA.

Original languageEnglish
Pages (from-to)49-54
Number of pages6
JournalConnective Tissue Research
Volume54
Issue number1
DOIs
Publication statusPublished - 2013
Externally publishedYes

Fingerprint

Intra-Articular Injections
Anterior Cruciate Ligament
Ligaments
Hyaluronic Acid
Osteoarthritis
Rats
Knee
Antiporters
Glutamic Acid
Cystine
Dialysis Solutions
Cartilage
Glutamates
Down-Regulation
Injections
Synovitis
Synovial Membrane
Synovial Fluid
Thigh
Association reactions

Keywords

  • Anterior cruciate ligament transaction
  • Cystine/glutamate antiporter system
  • Hyaluronic acid
  • Osteoarthritis
  • Prophylaxis

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Early intraarticular injection of hyaluronic acid attenuates osteoarthritis progression in anterior cruciate ligament-transected rats. / Tsai, Wei Yuan; Wu, Jia Lin; Liu, Chih Chung; Cherng, Chen Hwan; Tsai, Ru Yin; Jean, Yen Hsuan; Wong, Chih Shung.

In: Connective Tissue Research, Vol. 54, No. 1, 2013, p. 49-54.

Research output: Contribution to journalArticle

Tsai, Wei Yuan ; Wu, Jia Lin ; Liu, Chih Chung ; Cherng, Chen Hwan ; Tsai, Ru Yin ; Jean, Yen Hsuan ; Wong, Chih Shung. / Early intraarticular injection of hyaluronic acid attenuates osteoarthritis progression in anterior cruciate ligament-transected rats. In: Connective Tissue Research. 2013 ; Vol. 54, No. 1. pp. 49-54.
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abstract = "Subject: Hyaluronic acid (HA) is widely used to relieve the symptoms of osteoarthritis (OA). An association of reduction of glutamate content with the synovial fluid of OA rats was reported previously. Design: Anterior cruciate ligament transaction (ACLT) was performed on one knee in male Wistar rats, the other knee was assigned to sham control and HA or saline was injected intraarticularly into the ACLT knee from week 3 to week 7. Knee dialysate was collected for amino acid measurement at week 20. Morphology and histopathology of the femoral medial condyles and synovium were examined and evaluated using Mankin and synovitis scores. Results: HA injection provided better cartilage (3.38 ± 0.03 vs. 5.45 ± 0.0.02) and synovial condition (3 ± 0.02 vs. 6.03 ± 0.02) than saline controls. Moreover, HA injection reduced the concentration of glutamates in knee dialysates compared to saline controls (1.11 ± 0.14-folds and 2.21 ± 0.19-folds of the sham-operated knee, respectively). Cystine/glutamate antiporter system expression was significantly downregulated in the saline group, but not in the HA group (0.32 ± 0.08-folds and 0.71 ± 0.10-folds of the sham-operated knee, respectively). Conclusion: Early intraarticular injection of HA attenuates the progression of cartilage destruction in the ACLT knee, and the downregulation of the cystine/glutamate antiporter system was accompanied by the progression of OA.",
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AU - Jean, Yen Hsuan

AU - Wong, Chih Shung

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AB - Subject: Hyaluronic acid (HA) is widely used to relieve the symptoms of osteoarthritis (OA). An association of reduction of glutamate content with the synovial fluid of OA rats was reported previously. Design: Anterior cruciate ligament transaction (ACLT) was performed on one knee in male Wistar rats, the other knee was assigned to sham control and HA or saline was injected intraarticularly into the ACLT knee from week 3 to week 7. Knee dialysate was collected for amino acid measurement at week 20. Morphology and histopathology of the femoral medial condyles and synovium were examined and evaluated using Mankin and synovitis scores. Results: HA injection provided better cartilage (3.38 ± 0.03 vs. 5.45 ± 0.0.02) and synovial condition (3 ± 0.02 vs. 6.03 ± 0.02) than saline controls. Moreover, HA injection reduced the concentration of glutamates in knee dialysates compared to saline controls (1.11 ± 0.14-folds and 2.21 ± 0.19-folds of the sham-operated knee, respectively). Cystine/glutamate antiporter system expression was significantly downregulated in the saline group, but not in the HA group (0.32 ± 0.08-folds and 0.71 ± 0.10-folds of the sham-operated knee, respectively). Conclusion: Early intraarticular injection of HA attenuates the progression of cartilage destruction in the ACLT knee, and the downregulation of the cystine/glutamate antiporter system was accompanied by the progression of OA.

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