Early detection of antibodies against various structural proteins of the SARS-associated coronavirus in SARS patients

Ho Sheng Wu, Yueh Chun Hsieh, Ih Jen Su, Ting Hsiang Lin, Shu Chun Chiu, Yu Fen Hsu, Jih Hui Lin, Mei Ching Wang, Jeou Yuan Chen, Pei Wen Hsiao, Geen Dong Chang, Andrew H J Wang, Hsien Wei Ting, Chih Ming Chou, Chang Jen Huang

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

Severe acute respiratory syndrome (SARS), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in March 2003. Because of its relatively high transmissibility and mortality upon infection, probable SARS patients were quarantined and treated with special and intensive care. Therefore, instant and accurate laboratory confirmation of SARS-associated coronavirus (SARS-CoV) infection has become a worldwide interest. For this need, we purified recombinant proteins including the nucleocapsid (N), envelope (E), membrane (M), and truncated forms of the spike protein (S1-S7) of SARS-CoV in Escherichia coli. The six proteins N, E, M, S2, S5, and S6 were used for Western blotting (WB) to detect various immunoglobulin classes in 90 serum samples from 54 probable SARS patients. The results indicated that N was recognized in most of the sera. In some cases, S6 could be recognized as early as 2 or 3 days after illness onset, while S5 was recognized at a later stage. Furthermore, the result of recombinant-protein-based WB showed a 90% agreement with that of the whole-virus-based immunofluorescence assay. Combining WB with existing RT-PCR, the laboratory confirmation for SARS-CoV infection was greatly enhanced by 24.1%, from 48.1% (RT-PCR alone) to 72.2%. Finally, our results show that IgA antibodies against SARS-CoV can be detected within 1 week after illness onset in a few SARS patients.

Original languageEnglish
Pages (from-to)117-126
Number of pages10
JournalJournal of Biomedical Science
Volume11
Issue number1
DOIs
Publication statusPublished - 2004

Keywords

  • Antibody response
  • Recombinant proteins
  • SARS-CoV infection
  • Western blotting

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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