Abstract
It is well-documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high-strain dynamic compression to cell-seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short-term (3 or 9 h) or long-term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3-h of compression when compared with the free-swelling samples. Furthermore, long-term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high-strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 2009 |
| Externally published | Yes |
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Keywords
- Articular cartilage tissue engineering
- Chitosan
- Chondrocytes
- Dynamic compression
- Elastic scaffolds
- Gelatin
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering
Cite this
Dynamic compression modulates chondrocyte proliferation and matrix biosynthesis in chitosan/gelatin scaffolds. / Wang, Peng Yuan; Chow, Hsiang Hong; Lai, Juin Yih; Liu, Hsuan Liang; Tsai, Wei Bor.
In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 91, No. 1, 10.2009, p. 143-152.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dynamic compression modulates chondrocyte proliferation and matrix biosynthesis in chitosan/gelatin scaffolds
AU - Wang, Peng Yuan
AU - Chow, Hsiang Hong
AU - Lai, Juin Yih
AU - Liu, Hsuan Liang
AU - Tsai, Wei Bor
PY - 2009/10
Y1 - 2009/10
N2 - It is well-documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high-strain dynamic compression to cell-seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short-term (3 or 9 h) or long-term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3-h of compression when compared with the free-swelling samples. Furthermore, long-term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high-strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering.
AB - It is well-documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high-strain dynamic compression to cell-seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short-term (3 or 9 h) or long-term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3-h of compression when compared with the free-swelling samples. Furthermore, long-term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high-strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering.
KW - Articular cartilage tissue engineering
KW - Chitosan
KW - Chondrocytes
KW - Dynamic compression
KW - Elastic scaffolds
KW - Gelatin
UR - http://www.scopus.com/inward/record.url?scp=70049095440&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70049095440&partnerID=8YFLogxK
U2 - 10.1002/jbm.b.31384
DO - 10.1002/jbm.b.31384
M3 - Article
C2 - 19399846
AN - SCOPUS:70049095440
VL - 91
SP - 143
EP - 152
JO - Journal of Biomedical Materials Research
JF - Journal of Biomedical Materials Research
SN - 1552-4973
IS - 1
ER -