Abstract
It is well-documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high-strain dynamic compression to cell-seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short-term (3 or 9 h) or long-term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3-h of compression when compared with the free-swelling samples. Furthermore, long-term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high-strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering.
| Original language | English |
|---|---|
| Pages (from-to) | 143-152 |
| Number of pages | 10 |
| Journal | Journal of Biomedical Materials Research - Part B Applied Biomaterials |
| Volume | 91 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Oct 2009 |
| Externally published | Yes |
Keywords
- Articular cartilage tissue engineering
- Chitosan
- Chondrocytes
- Dynamic compression
- Elastic scaffolds
- Gelatin
ASJC Scopus subject areas
- Biomaterials
- Biomedical Engineering