Dynamic compression modulates chondrocyte proliferation and matrix biosynthesis in chitosan/gelatin scaffolds

Peng Yuan Wang, Hsiang Hong Chow, Juin Yih Lai, Hsuan Liang Liu, Wei Bor Tsai

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

It is well-documented that dynamical compression stimulates biosynthesis of extracellular biomacromolecules in cartilage explant or in chondrocyte/hydrogel systems. The object of this study was to apply high-strain dynamic compression to cell-seeded elastic scaffolds for articular cartilage tissue engineering. Rabbit chondrocytes had been cultured in chitosan/gelatin scaffolds for 3 days before dynamic compression. The chondrocyte/scaffold constructs were subjected to short-term (3 or 9 h) or long-term (6 h/day for 3 weeks) cyclic compression with 40% strain and 0.1 Hz. The expression of type II collagen and aggrecan was upregulated after 3-h of compression when compared with the free-swelling samples. Furthermore, long-term culture under dynamic compression facilitated cellular proliferation and deposition of glycosaminoglycan. Our results suggest that high-strain dynamic compression combined with elastic scaffolds might benefit articular cartilage tissue engineering.

Original languageEnglish
Pages (from-to)143-152
Number of pages10
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume91
Issue number1
DOIs
Publication statusPublished - Oct 2009
Externally publishedYes

Keywords

  • Articular cartilage tissue engineering
  • Chitosan
  • Chondrocytes
  • Dynamic compression
  • Elastic scaffolds
  • Gelatin

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering

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